High Carbohydrate High Fat Diet Induced Hepatic Steatosis and Dyslipidemia Were Ameliorated byPsidium guajavaLeaf Powder Supplementation in Rats

Abstract

Psidium guajava leaf is reported to contain many bioactive polyphenols which play an important role in the prevention and treatment of various diseases. Our investigation aimed to study the effect of Psidium guajava leaf powder supplementation on obesity and liver status by using experimental rats. To study the effects of guava leaf supplementation in high fat diet induced obesity, rats were randomly divided into four experimental groups (n=7), control (group I), control + guava leaf (group II), HCHF (group III), and HCHF + guava leaf (group IV). At the end of the experimental period (56 days), glucose intolerance, liver enzymes activities, antioxidant enzymes activities, and lipid and cholesterol profiles were evaluated. Our results revealed that guava leaf powder supplementation showed a significant reduction in fat deposition in obese rats. Moreover, liver enzyme functions were increased in high fat diet fed rats compared to the control rats significantly which were further ameliorated by guava leaf powder supplementation in high fat diet fed rats. High fat diet feeding also decreased the antioxidant enzyme functions and increased the lipid peroxidation products compared to the control rats. Guava leaf powder supplementation in high fat diet fed rats reduced the oxidative stress markers and reestablished antioxidant enzyme system in experimental animals. Guava leaf powder supplementation in high fat diet fed rats also showed a relative decrease in inflammatory cells infiltration and collagen deposition in the liver compared to the high fat diet fed rats. The present study suggests that the supplementation of guava leaf powder prevents obesity, improves glucose intolerance, and decreases inflammation and oxidative stress in liver of high carbohydrate high fat diet fed rats.

Amaranthus spinosus Attenuated Obesity-Induced Metabolic Disorders in High-Carbohydrate-High-Fat Diet-Fed Obese Rats

“…We found elevated levels of serum ALT (124.0 ± 3.24 U/L), AST (280.2 ± 46.66 U/L), and ALP (3599 ± 483.7 U/L) in HCHF diet-induced obese rats compared to the control rats (87.20 ± 7.95, 191.6 ± 18.58, and 1,825 ± 417.2 U/L, respectively). This complies with the results previously found in HCHF diet-induced obese rat model ( 4 , 23 , 28 , 32 ). Supplementation of powder, MeOH extract, and Aq.…”

“…In such a condition, hepatic gluconeogenesis remains unaffected and blood glucose level increases. Furthermore, with an unaltered supply of high energy diet, hepatic lipogenesis continues to occur that can lead to consequences ranging from simple steatosis to steatohepatitis and fibrosis ( 32 ). Disrupted lipid homeostasis also causes atherogenic dyslipidemia characterized by increased levels of LDL-C augmenting chances of cardiac complications ( 4 ).…”

“…Though genetic factors significantly contribute to the development of dyslipidemia, researchers have claimed diet rich in fat and carbohydrate as the most common cause (49,50). High-carbohydrate-highfat containing diet can also induce dyslipidemia in laboratory rats in a similar way to humans (4,23,32). In our study, significantly elevated serum levels of TC, TG, LDL-C, and VLDL-C, and decreased HDL-C level were found in HCHF diet-fed rats compared to control rats (Table 2), thus showing evidence for the development of atherogenic dyslipidemia.…”

Ameliorative effect of Psidium guajava (l.) leaf aqueous extract on aluminium nitrate-induced liver damage in female Wistar rats

“…This is consistent with the hepato-protective potential of Psidium guajava in earlier studies against erythromycin, carbon tetrachloride (CCl 4 ) liver damage in rats [14,19,20]. Histological evidence also suggests that guava leaf powder supplementation ameliorated the fat deposition in liver of high carbohydrate high fat diet fed rats [15]. The observed activity by Psidium guajava could be credited to the chemical compounds such as tannins, flavonoids, pentacyclic triterpenoids, guiajaverin, and quercetin which are present in the extract [21].…”

“…There are reports on hepato-protective effects of P. guajava leaf extracts [13][14][15] but, scanty reports are available on the effect of the aqueous extract of P.guajava leaf in ameliorating environmental toxicant exposed liver in rats.…”