High Cell Density Perfusion Culture of Insect Cells for Production of Baculovirus and Recombinant Protein

Klöppinger, Martina; Fertig, Georg; Fraune, Elisabeth; Miltenburger, H.G.

doi:10.1016/b978-0-7506-1103-9.50089-5

Cited by 11 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…High-density perfusion processes have been reported to be one of the attractive alternatives to a batch process to produce recombinant proteins with increased volumetric productivity (Jager, 1996). Internal (Deutschmann and Jager, 1994;Kloppinger et al, 1991;Schutz et al, 1991) and external (Caron et al, 1994;Massie et al, 1992;Reuveny et al, 1994) membrane perfusion systems have been used to grow insect cells to a cell density over 10 × 10 6 cells/mL (Cavegn and Bernard, 1992;Deramoudt et al, 1994;Guillaume et al, 1992;Jager et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

High-density perfusion culture of insect cells with a BioSep ultrasonic filter

Zhang

Collins

Chen

et al. 1998

Biotechnol. Bioeng.

View full text Add to dashboard Cite

The baculovirus/insect cell expression system has provided a vital tool to produce a high level of active proteins for many applications. We have developed a very high‐density insect cell perfusion process with an ultrasonic filter as a cell retention device. The separation efficiency of the filter was studied under various operating conditions. A cell density of over 30 million cells/mL was achieved in a controlled perfusion bioreactor and cell viability remained greater than 90%. Sf9 cells from a high‐density culture and a spinner culture were infected with two recombinant baculoviruses expressing genes for the production of human chitinase and monocyte‐colony inhibition factor. The protein yield on a cell basis from infecting high‐density Sf9 cells was the same as or higher than that from the spinner Sf9 culture. Virus production from the high‐density culture was similar to that from the spinner culture. The results show that the ultrasonic filter did not affect insect cells' ability to support protein expression and virus production following infection with baculovirus. The potential applications of the high‐density perfusion culture for large‐scale protein expression from Sf9 cells are also highlighted. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 59:351–359, 1998.

show abstract

Section: Introductionmentioning

confidence: 99%

High-density perfusion culture of insect cells with a BioSep ultrasonic filter

Zhang

Collins

Chen

et al. 1998

Biotechnol. Bioeng.

View full text Add to dashboard Cite

show abstract

“…The high-density culture of insect cells is essential to the maximization of productivity. To increase cell density, various techniques such as the periodic replacement of a medium, perfusion cultures [8], and immobilization [7] have all been used. Fed-batch operation has been widely used for the highdensity culture of microorganisms and animal cells.…”

Section: Introductionmentioning

confidence: 99%

Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution

Kim

Park

2000

Bioprocess Engineering

View full text Add to dashboard Cite

Amino acids rather than sugars are the primary limiting substrates for the culture of insect cells in a Grace's medium. When cultures are supplemented with amino acids, the yeastolate components other than the amino acids become the secondary limiting substrates. For the fed-batch culture of insect cells, a solution containing concentrated amino acids and yeastolate was supplied using an exponential feed¯ow rate calculated from mass balance equations. During the batch period the speci®c growth rate was 0.02 h )1 , whereas during the fed-batch period it was measured as 0.007 and 0.012 h )1 on the basis of the cell numbers and the dry cell weight, respectively. This difference in the speci®c growth rates in the fed-batch period is caused by an increase in the cell size during this period. Furthermore, in fed-batch cultures, dissolved oxygen was found to be a limiting factor for high cell-density cultures.

show abstract

Specialized Techniques

Freshney

2005

Culture of Animal Cells

101

View full text Add to dashboard Cite

High Cell Density Perfusion Culture of Insect Cells for Production of Baculovirus and Recombinant Protein

Cited by 11 publications

References 4 publications

High-density perfusion culture of insect cells with a BioSep ultrasonic filter

High-density perfusion culture of insect cells with a BioSep ultrasonic filter

Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution

Specialized Techniques

Contact Info

Product

Resources

About