High Contrast Upconversion Luminescence Targeted Imaging in Vivo Using Peptide-Labeled Nanophosphors

Xiong, Liqin; Chen, Zhigang; Tian, Qiwei; Cao, Tianye; Xu, Congjian; Li, Fuyou

doi:10.1021/ac901960d

Cited by 384 publications

(340 citation statements)

References 39 publications

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“…11,12 The use of near-infrared (NIR) light in PDT can afford greater penetration depths than that of VIS light because the absorbance for most biomolecules reaches a minimum in the NIR window (700-1,100 nm), 13 and the usefulness of the upconversion nanoparticles (UCNPs) that can convert NIR light to VIS light for noninvasive imaging of deep tissues, drug delivery, and PDT has been shown. [14][15][16][17][18][19][20] In this study, we loaded a commonly used second-generation photosensitizer, chlorin e6 (Ce6), onto silica-coated upconversion nanoparticles to form a supramolecular UCNPs-Ce6 complex used for NIR light-induced PDT of THP-1 macrophages, and we subsequently determined whether UCNPs-Ce6-mediated PDT induced apoptosis through the mitochondrial caspase pathway via ROS bursts, mitochondrial permeability transition pore (MPTP) opening, mitochondrial membrane potential (MMP) depolarization, and mitochondrial dysfunction in vitro.…”

Section: Introductionmentioning

confidence: 99%

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Zhu¹,

Wang²,

Zheng³

et al. 2015

IJN

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Atherosclerosis (AS) is the most vital cardiovascular disease, which poses a great threat to human health. Macrophages play an important role in the progression of AS. Photodynamic therapy (PDT) has emerged as a useful therapeutic modality not only in the treatment of cancer but also in the treatment of AS. The purpose of this study was to determine the molecular mechanisms underlying the activity of PDT, using mesoporous-silica-coated upconversion fluorescent nanoparticles encapsulating chlorin e6 (UCNPs-Ce6) in the induction of apoptosis in THP-1 macrophages. Here, we investigated the ability of UCNPs-Ce6-mediated PDT to induce THP-1 macrophage apoptosis by facilitating the induction of reactive oxygen species (ROS) and regulation of mitochondrial permeability transition pore (MPTP) to depolarize mitochondrial membrane potential (MMP). Both Bax translocation and the release of cytochrome C were examined using immunofluorescence and Western blotting. Our results indicated that the levels of ROS were significantly increased in the PDT group, resulting in both MPTP opening and MMP depolarization, which led to apoptosis. In addition, immunofluorescence and Western blotting revealed that PDT induced both Bax translocation and the release of cytochrome C, as well as upregulation of cleaved caspase-9, cleaved caspase-3, and cleaved poly(ADP-ribose) polymerase. Therefore, we demonstrated that UCNPs-Ce6-mediated PDT induces apoptosis in THP-1 macrophages via ROS bursts. The proapoptotic factor Bax subsequently translocates from the cytosol to the mitochondria, resulting in the MPTP opening and cytochrome C release. This study demonstrated the great potential of UCNPs-Ce6-mediated PDT in the treatment of AS.

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Section: Introductionmentioning

confidence: 99%

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Zhu¹,

Wang²,

Zheng³

et al. 2015

IJN

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“…As noted above, the narrow line luminescence, long ES lifetimes and photostability of Ln III -doped UCNPs make these particularly well suited for biological imaging. In this context, there have been some really exciting developments in cellular imaging [103][104][105][106][107] and in vivo imaging with small animals [103,104,[108][109][110][111]. For example, strongly fluorescent core : shell β-NaYF 4 :Yb,Er UCNPs, coated with a thin layer of SiO 2 and modified with amino groups, were covalently linked to rabbit anti-CEA8 antibodies.…”

Section: Uncaging Using Upconversion Nanoparticles (A) Introduction Tmentioning

confidence: 99%

Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Garcia

Zhang

Ford

2013

Phil. Trans. R. Soc. A.

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Multi-photon excitation allows one to use tissue transmitting near-infrared (NIR) light to access excited states with energies corresponding to single-photon excitation in the visible or ultraviolet wavelength ranges. Here, we present an overview of the application of both simultaneous and sequential multi-photon excitation in studies directed towards the photochemical delivery (‘uncaging’) of bioactive small molecules such as nitric oxide (NO) to physiological targets. Particular focus will be directed towards the use of dyes with high two-photon absorption cross sections and lanthanide ion-doped upconverting nanoparticles as sensitizers to facilitate the uncaging of NO using NIR excitation.

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“…Then, the MA-UCNPs were linked with the thiolated cyclopeptide (Arg-Gly-Asp-Phe-Lys(mpa)) for targeted imaging. 67,70 Maeda team 71 structured a MAfunctionalized Er-Y 2 O 3 by reacting MA-PEG-NHS with APTES-Er-Y 2 O 3 . So the MA-functionalized UCNPs could be linked with cyclic RGD peptide to speci¯cally target to integrin v 3 which is highly expressed in the tumor cell surface.…”

Section: Ma Group Modi¯cationmentioning

confidence: 99%

Upconversion nanoparticle as a theranostic agent for tumor imaging and therapy

Fang

Wei

2016

J. Innov. Opt. Health Sci.

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Upconversion nanoparticles (UCNPs) as a promising material are widely studied due to their unique optical properties. The material can be excited by long wavelength light and emit visible wavelength light through multiphoton absorption. This property makes the particles highly attractive candidates for bioimaging and therapy application. This review aims at summarizing the synthesis and modi¯cation of UCNPs, especially the applications of UCNPs as a theranostic agent for tumor imaging and therapy. The biocompatibility and toxicity of UCNPs are also further discussed. Finally, we discuss the challenges and opportunities in the development of UCNP-based nanoplatforms for tumor imaging and therapy.

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High Contrast Upconversion Luminescence Targeted Imaging in Vivo Using Peptide-Labeled Nanophosphors

Cited by 384 publications

References 39 publications

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Upconversion nanoparticle-mediated photodynamic therapy induces THP-1 macrophage apoptosis via ROS bursts and activation of the mitochondrial caspase pathway

Multi-photon excitation in uncaging the small molecule bioregulator nitric oxide

Upconversion nanoparticle as a theranostic agent for tumor imaging and therapy

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