High-density GBS-based genetic linkage map construction and QTL identification associated with leaf cuticular wax, adaxial stomatal density and leaf water retention capacity in banana

Sampangi-Ramaiah, Megha H.; Shivashankara, Kodthalu Seetharamaiah; Rekha, Ajitha; Laxman, Ramanna Hunashikatti; E, Dayanandhi; Ravishankar, Kundapura.V.

doi:10.1016/j.scienta.2023.112205

Cited by 2 publications

(1 citation statement)

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“…GBS has become a cost-effective approach for genotyping large numbers of samples, especially the mapping populations that are required for constructing linkage maps. GBS has been employed for constructing genetic linkage maps in several fruit tree species including apple ( Oh et al., 2023 ), Japanese plum ( Battistoni et al., 2022 ), Korean pears ( Han et al., 2019 ), banana ( Sampangi-Ramaiah et al., 2023 ) and in the guava’s closest related species eucalyptus ( Zhu et al., 2023 ). In our study, GBS has been applied for the first time in SNP calling in guava mapping populations which enabled us to identify 119,343 SNPs in both parents and hybrids.…”

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confidence: 99%

A high-density linkage map construction in guava (Psidium guajava L.) using genotyping by sequencing and identification of QTLs for leaf, peel, and pulp color in an intervarietal mapping population

Mathiazhagan,

Elangovan,

Chinnaiyan

et al. 2024

Front. Plant Sci.

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Psidium guajava L. is an important fruit crop in the tropical and subtropical regions of the world. The advanced breeding methods are not employed for important commercial traits like peel and pulp color, seed hardiness, fruit size, etc., due to the scarcity of genome-wide molecular markers and high-density linkage maps. In this study, we employed single-nucleotide polymorphism (SNP) markers and identified quantitative trait loci (QTL) regions that are associated with color traits of leaf, peel, and pulp in the guava intervarietal mapping population. The mapping population was developed from the contrasting genotypes of fruit and leaf color. Variations in color among the segregating hybrids were recorded both visually and using a Color reader. A high-density linkage map of guava was constructed using the SNP markers from genotyping by sequencing (GBS) of 150 hybrid individuals of the cross ‘Arka Poorna’ (green) x ‘Purple Local’ (purple). The integrated linkage map consisted of 1426 SNPs mapped on 11 linkage groups (LG), spanning a total distance of around 730 cM with an average of 129.6 markers per LG. Through QTL analysis for color traits, a minor QTL region was identified for visually scored leaf color and peel color on LG1, whereas a major QTL was detected for pulp color in LG4. The Hunter color values (L* and, a*) also had major QTLs with overlapping marker intervals for leaf and peel colors, establishing the association of SNP markers to the trait. The QTLs harbored genes and transcription factors involved in lycopene and anthocyanin pigment biosynthesis. This is the first report of a high-density linkage map based on SNP markers in guava and QTL mapping for color characters in leaf, fruit peel and pulp. The genotyping information generated in this study can aid in genetic engineering and marker-assisted breeding in guava.

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