High-density, microsphere-based fiber optic DNA microarrays

Epstein, Jason R.; Leung, Amy P K; Lee, Kyong Hoon; Walt, David R.

doi:10.1016/s0956-5663(03)00021-6

Cited by 109 publications

(62 citation statements)

References 24 publications

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“…This array platform provides many advantages over other array formats. These microarrays contain the smallest feature sizes so that it is believed that the fabrication protocol enables, in the future, their expansion into single cell-based assays (Epstein et al 2003 (Eberwine, 1992;Eberwine, 1996). The specific profile has extended from single-gene expression to multiplex PCR, from regular expression to in situ rtPCR, and from routine rtPCR to realtime rtPCR.…”

Section: Methods Of Genomic Expression From Single Cellsmentioning

confidence: 99%

A strategy to identify genomic expression at single-cell level or a small number of cells

2005

Electro Journal of Biotech

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Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is essential for scientists and physicians to develop a strategy to identify genomic expression profiles for a single-cell or for small numbers of cells. In this article, we review recently developed methods that enable functional genomic analysis at the single-cell or multicell level. In addition, the paper will review different techniques of single-cell genomic expression at the level of DNA, mRNA, protein and post-translational modifications.

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Section: Methods Of Genomic Expression From Single Cellsmentioning

confidence: 99%

A strategy to identify genomic expression at single-cell level or a small number of cells

2005

Electro Journal of Biotech

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“…Because the instrumentation required is sophisticated and expensive, gene-chip technology is best suited for laboratory applications. Cases in which large numbers of genes or sequences need to be simultaneously sampled, as in transcriptional profiling or SNP discovery, are well suited to gene-chip analysis [7][8][9][10] . Clinical diagnostics do not generally require this massive data accumulation.…”

Section: Principles Of Biosensor Functionmentioning

confidence: 99%

Electrochemical DNA sensors

2003

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“…Microsphere-based fiber-optic arrays (Illumina; www.illumina.com) provide many advantages over other array-based methods [2]: higher sensor packing density, smaller assay sample volumes, increased array reusability, flexible array design, and reduced false-positives and false-negatives [50]. Previous work has demonstrated that the microsphere-based fiber-optic array can detect as few as 600 target DNA molecules and is sensitive enough to discriminate a single-base mismatch from a perfect match [49,51].…”

Section: Liquid Arraysmentioning

confidence: 99%

Introduction to Microarray‐Based Detection Methods

Schrenzel

Kostić²,

Bodrossy

et al. 2009

Detection of Highly Dangerous Pathogens

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Microarrays consist of an orderly arrangement of probes (oligonucleotides, DNA fragments, proteins, sugars or lectins) attached to a solid surface. The main advantages of microarray technology are high throughput, parallelism, miniaturization, speed and automation. Despite the fact that microarray analysis is a relatively novel technology, with the publication of the first microarray studies in 1995 [101,152], it is now broadly applied and the milestone of nearly 5000 published microarray papers was recorded in 2004 [80]. The scientific and technological background discussed here will be limited to DNA microarrays, excluding the new evolving fields of protein microarrays and glycomics [140].Analogous to antigen-antibody interactions on immunoarrays, DNA microarrays rely on sequence complementarity of the two strands. They put in practice the fundamentals of complementary base-pairing (hybridization) that were first described by Ed Southern [162]. In general, the strategy of microarray hybridization is reversed to that of a standard dot-blot, leading to recurring confusion in the nomenclature. Therefore, it has been suggested to describe tethered nucleic acid as the probe and free nucleic acid as the target [134].Earlier studies on duplex melting and reformation, carried out on DNA solutions, have provided the basic knowledge about the reaction kinetics. Furthermore, a method for computational determination of the melting point as a function of nucleic acid composition and salt concentration was established [6,148,149]. Much of the pioneering work can be linked to the use of nitrocellulose membranes [69], dot-blots [84] and Southern blots [162]. Development of cDNA or oligonucleotide arrays was possible by combined innovations in micro-engineering, molecular biology [33,120,123,156,163,164] and bioinformatics [59]. The real breakthrough in microarray technology was initiated by two key innovations: the use of non-porous solid supports (such as glass and silicon) and the development of methods for highdensity synthesis of oligonucleotides directly onto the microarray surface [59].

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High-density, microsphere-based fiber optic DNA microarrays

Cited by 109 publications

References 24 publications

A strategy to identify genomic expression at single-cell level or a small number of cells

A strategy to identify genomic expression at single-cell level or a small number of cells

Electrochemical DNA sensors

Introduction to Microarray‐Based Detection Methods

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