High-density multi-fiber photometry for studying large-scale brain circuit dynamics

Sych, Yaroslav; Chernysheva, Maria; Sumanovski, Lazar T.; Helmchen, Fritjof

doi:10.1038/s41592-019-0400-4

Cited by 180 publications

(120 citation statements)

References 61 publications

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“…Here, we combine an electrophysiological approach with GECI-based fiber photometry to simultaneously monitor both field potentials and calcium transients in freely behaving mice. Fiber photometry is an imaging method used to monitor fluorescent signals via an implanted fiber optic cannula (Adelsberger et al, 2005;Lutcke et al, 2010;Kim et al, 2016;Sych et al, 2019). Although it is still invasive, the diameter of the cannula is thinner than an endoscope.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice

et al. 2020

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In vivo electrophysiology is the gold standard technique used to investigate subsecond neural dynamics in freely behaving animals. However, monitoring celltype-specific population activity is not a trivial task. Over the last decade, fiber photometry based on genetically encoded calcium indicators (GECIs) has been widely adopted as a versatile tool to monitor cell-type-specific population activity in vivo. However, this approach suffers from low temporal resolution. Here, we combine these two approaches to monitor both sub-second field potentials and cell-type-specific population activity in freely behaving mice. By developing an economical custommade system and constructing a hybrid implant of an electrode and a fiber optic cannula, we simultaneously monitor artifact-free mesopontine field potentials and calcium transients in cholinergic neurons across the sleep-wake cycle. We find that mesopontine cholinergic activity co-occurs with sub-second pontine waves, called P-waves, during rapid eye movement sleep. Given the simplicity of our approach, simultaneous electrophysiological recording and cell-type-specific imaging provides a novel and valuable tool for interrogating state-dependent neural circuit dynamics in vivo.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice

et al. 2020

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“…Apart from neural electrophysiology probes, the MPSA microdrive could also be used for other biological as well as non-biological probe translation tasks 67,68 . For example, the micro-grippers could be sized to accommodate hypodermic needles, optical fibers and ultrasonic transducers and sensors of a variety of scales.…”

Section: Discussionmentioning

confidence: 99%

Robotic Multi-Probe-Single-Actuator Inchworm Neural Microdrive

Smith

Kolb

Tanaka

et al. 2020

Preprint

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Electrophysiology is one of the major experimental techniques used in neuroscience. The favorable spatial and temporal resolution as well as the increasingly larger site counts of brain recording electrodes contribute to the popularity and importance of electrophysiology in neuroscience. Such electrodes are typically mechanically placed in the brain to perform acute or chronic freely moving animal measurements. The micro positioners currently used for such tasks employ a single translator per independent probe being placed into the targeted brain region, leading to significant size and weight restrictions. To overcome this limitation, we have developed a miniature robotic multi-probe neural microdrive that utilizes novel phase-change-material-filled resistive heater micro-grippers. The microscopic dimensions, gentle gripping action, independent electronic actuation control, and high packing density of the grippers allow for micrometer-precision independent positioning of multiple arbitrarily shaped parallel neural electrodes with only a single piezo actuator in an inchworm motor configuration. This multi-probe-single-actuator design allows for significant size and weight reduction, as well as remote control and potential automation of the microdrive. We demonstrate accurate placement of multiple independent recording electrodes into the CA1 region of the rat hippocampus in vivo in acute and chronic settings. Thus, our robotic neural microdrive technology is applicable towards basic neuroscience and clinical studies, as well as other multi-probe or multi-sensor micro-positioning applications.

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“…One limitation to progress on this front has been the technological bottleneck preventing widespread activity measurements across large areas of deep brain tissue, like the striatum. Recent advances in large‐scale recording methods that can access subcortical structures such as high density fiber photometry and electrophysiology (Hamid et al, ; Jun et al, ; Pisano et al, ; Sych, Chernysheva, Sumanovski, & Helmchen, ) offer promise for uncovering novel functional dynamics in the striatum. These approaches, combined with brain‐wide circuit‐ and network‐level functional imaging (Decot et al, ; Heilbronner et al, ; Lohani, Poplawsky, Kim, & Moghaddam, ) will also be important for translation of rodent findings to humans. Functions of neurotransmitter co‐release from dopamine neurons: Many dopamine neurons co‐release other neurotransmitters, including GABA and glutamate (Morales & Margolis, ; Morales & Root, ; Tecuapetla et al, ; Tritsch, Ding, & Sabatini, ) and co‐release varies depending on dopamine neuron subpopulation.…”

Section: Circuit and Subregional Functional Heterogeneitymentioning

confidence: 99%

Heterogeneity in striatal dopamine circuits: Form and function in dynamic reward seeking

Collins

Saunders

2020

J of Neuroscience Research

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The striatal dopamine system has long been studied in the context of reward learning, motivation, and movement. Given the prominent role dopamine plays in a variety of adaptive behavioral states, as well as diseases like addiction, it is essential to understand the full complexity of dopamine neurons and the striatal systems they target. A growing number of studies are uncovering details of the heterogeneity in dopamine neuron subpopulations. Here, we review that work to synthesize current understanding of dopamine system heterogeneity across three levels, anatomical organization, functions in behavior, and modes of action, wherein we focus on signaling profiles and local mechanisms for modulation of dopamine release. Together, these studies reveal new and emerging dimensions of the striatal dopamine system, informing its contribution to dynamic motivational and decision-making processes.

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High-density multi-fiber photometry for studying large-scale brain circuit dynamics

Cited by 180 publications

References 61 publications

Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice

Simultaneous Electrophysiology and Fiber Photometry in Freely Behaving Mice

Robotic Multi-Probe-Single-Actuator Inchworm Neural Microdrive

Heterogeneity in striatal dopamine circuits: Form and function in dynamic reward seeking

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