High diluted and dynamised follicle stimulating hormone modulates steroid production in isolated porcine preantral follicles cultured in vitro

Lima, Laritza Ferreira de; Rubessa, M.; Rocha, Rebeca Magalhães Pedrosa; Winters, Rebecca; Milner, Derek J.; Campello, C.C.; Figueiredo, J.R.; Wheeler, Matthew B.

doi:10.1016/j.homp.2017.03.004

Cited by 7 publications

(8 citation statements)

References 33 publications

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“…Ovaries (n = 120) from prepubertal gilts were collected at a local abattoir and transported to the laboratory in physiological saline at 30-35°C. The ovaries were washed with 70% ethanol for 10 s, followed by a wash with saline solution supplemented with penicillin (100 μg/mL) and streptomycin (100 μg/mL; Lima et al, 2017;Yu et al, 2018).…”

Section: Ovary Collectionmentioning

confidence: 99%

“…Only those follicles showing an intact basement membrane, with bright and homogeneous granulosa cells and an absence of morphological signs of degeneration, were classified as morphologically normal. Every 2 days of culture (days 0, 2, and 4), the following characteristics were analyzed in the morphologically normal follicles: (1) antral cavity formation, defined as the emergence of a visible translucent cavity within the granulosa cell layers, and (2) the diameter of morphologically normal follicles, measured from the basement membrane, which included two perpendicular measures of each preantral follicle, with the aid of Carl Zeiss Microscopy BmbH 2011 and analyzed by Zen Lite program under 100x magnification (Lima et al, 2017).…”

Section: Morphological Evaluation Of Follicle Developmentmentioning

confidence: 99%

“…Spent medium was collected at days 2 and 4 and stored at -80°C for subsequent estradiol (E2), progesterone (P4) and testosterone (T) assays (Lima et al, 2017). Steroid concentrations were determined by Enzyme-Linked ImmunoSorbent Assay (ELISA) kits: Arbor Assays K030-H1 (E2), K025-H1 (P4) and K032-H1 (T).…”

Section: Hormone Assaysmentioning

confidence: 99%

“…However, similar success has not been reported in large mammals. In pigs, to our knowledge, in vitro experiments with preantral follicles are few and with limited success (Wu, Emery, & Carrell, 2001;Mao et al, 2002;Mao et al, 2004;Wu, Xu, & Wang, 2007;Tasaki, Iwata, Sato, Monji, & Kuwayama, 2013;Lima et al, 2017;Cao et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

Rocha

Rubessa

Lima

et al. 2021

RSD

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To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (α-MEM) as a base medium, and to evaluate the effects of Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating Hormone (FSH) on the in vitro development and steroid production of isolated porcine preantral follicles. Porcine secondary follicles were cultured in NCSU23 or α-MEM media for 4 days. Once α-MEM was determined to be the optimal culture medium, secondary follicles were cultured in α-MEM alone or supplemented with FSH (1.5 ng/mL), AMH (50 ng/mL) or the combination of the two hormones. Follicle development was evaluated by measuring follicular growth, morphology and hormone production. There was no difference between the media NCSU23 and α-MEM in terms of follicle survival and growth (P > 0.05). However, at day 2, the antrum formation rate tended to be (P < 0.074) higher in α-MEM than NCSU23. At day 4 of culture, the estradiol and progesterone secretion were higher in α-MEM than NCSU23 (P < 0.01), while the opposite was observed for testosterone (P < 0.01). The addition of AMH and/or FSH did not affect follicular survival and growth. Nevertheless, the secretion of estradiol and progesterone induced by FSH was reduced with AMH (P < 0.01). α-MEM is a more effective base medium than NCSU23 for the in vitro follicular development of pig preantral follicles and AMH reduces the steroid production induced by FSH.

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Section: Ovary Collectionmentioning

confidence: 99%

Section: Morphological Evaluation Of Follicle Developmentmentioning

confidence: 99%

Section: Hormone Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

Rocha

Rubessa

Lima

et al. 2021

RSD

Self Cite

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“…In this sense, the in vitro models, for example the IVM, could represent an excellent tool to solve such intriguing issues. Concerning reproduction, few studies investigated the effects of homeopathic medicinal products on the in vitro preantral follicle survival and development (sheep – Lima et al, 2016 and pig - Lima et al, 2017 ). However, to the best of our knowledge, there are no reports on the in vitro effects of those products on the in vitro maturation of oocytes.…”

Section: Introductionmentioning

confidence: 99%

Ultra-diluted Folliculinum 6 cH impairs ovine oocyte viability and maturation after in vitro culture

et al. 2020

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This study investigated the effect of Folliculinum 6 cH on the oocyte meiosis resumption and viability rates, progesterone production and mitochondrial activity after in vitro maturation of cumulus-oocyte complexes (COCs) in sheep. Sheep ovaries were collected at a local slaughterhouse and COCs were recovered by slicing technique. The selected COCs were maturated in TCM199 (Control treatment), or control medium supplemented with 0.05% ethanol (v/v) (the vehicle of the homeopathic preparation-Ethanol treatment) or with Folliculinum 6 cH. After 24 h of in vitro maturation (IVM), oocytes were mechanically denuded and incubated with Hoechst 33342 and MitoTracker (0.5 μM) Orange CMTMRos for analysis of viability and chromatin configuration, and mitochondrial activity, respectively. The results showed that Folliculinum 6 cH addition increased oocyte degeneration and reduced meiotic resumption compared to the control (P < 0.05). Interestingly, the percentages meiotic resumption and oocyte maturation were lower in the Folliculinum 6 cH treatment compared to its vehicle (Ethanol treatment) (P < 0.05). On the other hand, when the treatments were compared, higher mitochondrial activity was observed in the Ethanol treatment (P < 0.05). In conclusion, contrary to its vehicle, the addition of Folliculinum 6 cH to the IVM medium promoted oocyte degeneration and affected negatively the mitochondrial distribution, impairing meiosis resumption.

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Ultra-diluted/dynamized doxorubicin reduces the toxicity caused by doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue

Lima de Andrade,

Palomino,

de Queiroz

et al. 2024

Reproductive Toxicology

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High diluted and dynamised follicle stimulating hormone modulates steroid production in isolated porcine preantral follicles cultured in vitro

Cited by 7 publications

References 33 publications

Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

Effect of base media, FSH and anti-Müllerian hormone (AMH) alone or in combination on the growth of pig preantral follicles in vitro

Ultra-diluted Folliculinum 6 cH impairs ovine oocyte viability and maturation after in vitro culture

Ultra-diluted/dynamized doxorubicin reduces the toxicity caused by doxorubicin during the in vitro culture of pig preantral follicles enclosed in ovarian tissue

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