High dynamic range detection of Chlamydia trachomatis growth by direct quantitative PCR of the infected cells

Eszik, Ildikó; Lantos, Ildikó; Önder, Kamil; Somogyvári, Ferenc; Burián, Katalin; Endrész, Valéria; Virók, Dezső

doi:10.1016/j.mimet.2015.11.010

Cited by 25 publications

(44 citation statements)

References 24 publications

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“…5 log 10 (Eszik et al . ). Different from the qPCR results, the ChlamyCount method also showed a limited chlamydial growth inhibition in the case of iodine aqueous solution and borax.…”

Section: Resultsmentioning

confidence: 97%

“…For chlamydial growth measurement we used the recently published direct qPCR‐based approach (Eszik et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…To test the antichlamydial activity of frequently used antiseptics, we infected HeLa cervical epithelial cells with C. trachomatis in the presence of iodine aqueous solution, povidone-iodine, chlorhexidine and borax. For chlamydial growth measurement we used the recently published direct qPCR-based approach (Eszik et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Impact of antiseptics on Chlamydia trachomatis growth

Párducz

Eszik

Wagner

et al. 2016

Lett Appl Microbiol

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We measured the antichlamydial effects of various antiseptics. These antiseptics are being used for the treatment of bacterial vaginosis, but their effect on the bacterial vaginosis-related sexually transmitted infections, particularly the most frequent Chlamydia trachomatis (C. trachomatis) infections has not been investigated. We showed that povidone-iodine (Betadine) inhibited the chlamydial growth in concentrations that was not toxic to the epithelial cells. We concluded that due to its additional antichlamydial effect, povidone-iodine could be a preferable antiseptic in bacterial vaginosis treatment.

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“…5 log 10 (Eszik et al . ). Different from the qPCR results, the ChlamyCount method also showed a limited chlamydial growth inhibition in the case of iodine aqueous solution and borax.…”

Section: Resultsmentioning

confidence: 97%

“…For chlamydial growth measurement we used the recently published direct qPCR‐based approach (Eszik et al . ).…”

Section: Introductionmentioning

confidence: 97%

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Impact of antiseptics on Chlamydia trachomatis growth

Párducz

Eszik

Wagner

et al. 2016

Lett Appl Microbiol

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show abstract

“…We used the recently published direct qPCR methods (Eszik et al . ; Virók et al . ) to determine the antimicrobial activity of the NPs on C. trachomatis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The mixed cell lysates were used as templates directly in the C. trachomatis and HSV‐2‐specific qPCRs, as described previously (Eszik et al . ; Virók et al . ).…”

Section: Methodsmentioning

confidence: 99%