High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium

Tallón, Carlos I.; Porras, I.; Pérez‐Tornero, O.

doi:10.1007/s11627-012-9476-6

Cited by 22 publications

(17 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are consistent with those of Tallon et al (2013) and Esmaeilnia & Dehestani (2015). Amgai et al (2016) found that shooting from explants was significantly higher (71.72%) on medium level of the BAP (0.5 mg/L) and IAA (0.2 mg/L) using in-vitro seedling stem as explant of mandarin orange.…”

Section: Effect Of 2mg/l Ba Combination With Different Naa Concentratsupporting

confidence: 92%

Establishment of in Direct Propagation of Mandarin (Citrus reticulata L.) Using Tissue Culture

Badr-Elden

2017

Egypt. J. Bot.

View full text Add to dashboard Cite

Section: Effect Of 2mg/l Ba Combination With Different Naa Concentratsupporting

confidence: 92%

Establishment of in Direct Propagation of Mandarin (Citrus reticulata L.) Using Tissue Culture

Badr-Elden

2017

Egypt. J. Bot.

View full text Add to dashboard Cite

“…At this concentration, 94.3% of the cultivated calli produced shoot buds with an average number of 4.3 buds/explants. Similar results were also reported for Citrus macrophylla and C. aurantium, in which the number of shoots was superior in culture medium supplemented with 2 and 3 mg L -1 BA, respectively, compared to treatments supplemented in conjunction with KIN (Tallón et al, 2013). In Punica granatum, the greatest formation of adventitious shoots from cotyledonary explants was also obtained in a medium supplemented with 2 mg L -1 BA (Parmar, 2012).…”

Section: Resultssupporting

confidence: 85%

Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana

et al. 2019

View full text Add to dashboard Cite

Understanding the in vitro performance of royal poinciana explants cultured in media supplemented with different types and concentrations of cytokinins may aid in the optimization of regeneration systems established for this woody species. In the present study we evaluate the in vitro performance of royal poinciana cotyledonary explants cultured in the presence of high concentrations of 6-benzyladenine (BA) and kinetin (KIN). Cotyledonary segments obtained from in vitro germinated seedlings were inoculated in Murashige and Skoog (MS) medium, supplemented with different concentrations (1, 2, 4, 8 mg L-1) of BA or KIN. In the control treatment, no plant growth regulators (PGRs) were added. After 40 days of culture, regardless of the concentration used, the treatments supplemented with BA presented higher calli percentage and fresh mass compared to treatments supplemented with KIN. Adventitious shoots were mainly observed in BA-treatments. Histological analysis showed that adventitious shoots formed at the periphery of callus formed from mesophyll cells in the regions of the explant sectioning. The results obtained provide new information for the establishment of a micropropagation system for royal poinciana, an important ornamental tree species.

show abstract

“…With respect to the 'Indio', 'Riverside' and 'San Diego' citrandarins and the hybrids CSM × (RL × TR)-059 and CSM × TRBK Colombia, the good performance of WPM in the micropropagation coincides with the results obtained by Tallón et al (2013) utilizing explants from adult plants of 'Alemow' (C. macrophylla Wester) and 'Sour' orange (Citrus × aurantium L.), for which WPM produced a higher percentage of explants recovered and number of shoots regenerated per explant than did MS medium.…”

Section: Resultssupporting

confidence: 70%

“…In citrus, as is the case of many other plant species groups, the success of any in vitro regeneration system depends on various factors, such as the incubation conditions, choice of culture medium, application of growth regulators and their concentrations, and type of explant. The genotype also has a strong influence on the tissue culture results (Cervera, A. Navarro, L. Navarro, & Peña, 2008;Tallón, Porras, & Pérez, 2013).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Santiago¹,

Santos²,

Lêdo³

et al. 2019

JAS

View full text Add to dashboard Cite

Micropropagation is a method that enables in vitro cloning of cultivated plants, irrespective of their potential to produce seeds and their polyembryony rate, guaranteeing genetic identity and absence of phytosanitary problems of the propagated plants if the multiplication process is conducted suitably. In this respect, citrus rootstocks of agronomic interest were micropropagated in Wood Plant Medium (WPM). The experiment was conducted in the municipality of Cruz das Almas, in the Recôncavo Baiano region of Bahia state, Brazil, with 10 rootstocks: ‘Indio’, ‘Riverside’ and ‘San Diego’ citrandarins, ‘Sunki Tropical’ mandarin, and the hybrids RL × TR-001, FRL × (RL × TR)-005, CSM × (RL × TR)-059, TRH-051, TRH-069 and CSM × TRBK-Colombia. Nodal segments of these genotypes, with approximate length of 1 cm, were used. The number of plants formed per explant was evaluated 180 days after inoculation. The experimental design was completely randomized, with ten treatments and 12 repetitions, the data obtained were submitted to analysis of variance (F-test) and the grouped means were analyzed by the Scott-Knott test, in both cases a 5% probability. In vitro culture of explants of the ten genotypes studied presented positive responses to micropropagation in the WPM medium, once the explants of all genotypes showed direct regeneration and formed plants. The citrandarin 'Indio' he stood out with the highest average of explants by plant (4.20).

show abstract

High efficiency in vitro organogenesis from mature tissue explants of Citrus macrophylla and C. aurantium

Cited by 22 publications

References 54 publications

Establishment of in Direct Propagation of Mandarin (Citrus reticulata L.) Using Tissue Culture

Establishment of in Direct Propagation of Mandarin (Citrus reticulata L.) Using Tissue Culture

Kinetin and 6-benzyladenine induce different morphogenetic responses in cotyledonary segments of royal poinciana

Micropropagation of Different Citrus Rootstocks Using WPM Medium Culture

Contact Info

Product

Resources

About