High-efficiency nano/micro-reactors for protein analysis

Li, Yixin; Liang, Yan; Liu, Yun; Qian, Kun; Liu, Bin; Yang, Pengyuan; Liu, Baohong

doi:10.1039/c4ra12333f

Cited by 37 publications

(20 citation statements)

References 166 publications

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“…9 Within this area, the field of micro-and nanoreactors, which consists in the fabrication of materials with defined patterned areas containing compounds with catalytic activity, is getting more and more attention. 10 Microreactors have allowed for integrating the exact conditions of a conventional reactor in the macroscale but in a reduced volume. Advantages include less consumption of material, improved contact between the diverse reactants and increased control of heat and mass transfer, which leads to an increase of the conversion.…”

Section: ■ Introductionmentioning

confidence: 99%

Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors

León

Garnier

Jierry

et al. 2015

ACS Appl. Mater. Interfaces

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Herein, we report the fabrication of microstructured porous surfaces with controlled enzymatic activity by combining the breath figures and the layer-by-layer techniques. Two different types of porous surfaces were designed based on fluorinated and carboxylated copolymers in combination with PS, using poly(2,3,4,5,6-pentafluorostyrene)-b-polystyrene (PS5F31-b-PS21) and polystyrene-b-poly(acrylic acid) (PS19-b-PAA10) block copolymers, respectively. For comparative purposes, flat surfaces having similar chemistry were obtained by spin-coating. Poly(sodium 4-styrenesulfonate)/poly(allylamine hydrochloride) (PSS/PAH) multilayers incorporating alkaline phosphatase (ALP) were built on these porous surfaces to localize the enzyme both inside and outside of the pores using PS/PS5F31-b-PS21 surfaces and only inside the pores on PS/PS19-b-PAA10 surfaces. A higher catalytic activity of ALP (about three times) was obtained with porous surfaces compared to the flat ones. The catalysis happens specifically inside the holes of PS/PS19-b-PAA10surfaces, where ALP is located. This opens the route for applications in microreactors.

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Section: ■ Introductionmentioning

confidence: 99%

Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors

León

Garnier

Jierry

et al. 2015

ACS Appl. Mater. Interfaces

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“…3,16,41 MS-based proteomics is an advanced technology interpreting encoded information in the genomes. 35,42,43 The approach has been successful in the case of small sets of proteins isolated in specific functional contexts. 44 Detecting patterns of a differentially expressed proteins clinical samples shows the potential to diagnose the presence and stage of many diseases such as cancer.…”

Section: Detection Of Proteins/peptides For Proteomic Analysismentioning

confidence: 99%

A coming era of precision diagnostics based on nano-assisted mass spectrometry

Qian

Wan

Gurav

et al. 2018

PRNANO

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Abbreviations:• SNP single-nucleotide polymorphism • qPCR quantitative real-time polymerase chain reaction • a6A N6-allyladenosine • RT reverse transcriptase • CyTOF mass cytometry by time-of-flight • Pre-DC dendritic cells precursors Abstract Precision diagnostics relies on omic analysis by mass spectrometry to overcome the limitation in accuracy by an individual biomarker, due to the complex nature of diseases. Recent development in nanotechnology markedly enhanced sample treatment and detection efficiency of this method. Herein, we foresee a coming era of precision diagnostics based on nano-assisted mass spectrometry. Some important progress in the field includes detection of (1) nucleic acids for genetic analysis; (2) proteins/peptides for proteomic analysis; and (3) small molecules for metabolic analysis. We anticipate that this review will be a reminder for both young and experienced researchers about the future of diagnostics and call for attention worldwide.Prnano.com,

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“…One of the most interesting microbioreactor techniques is the investigation of behavior and cell growth in different concentration gradient profiles. Differently, conventional assays such as bench scale bioreactors and shaking flasks depend on stepwise dilution of a concentrated nutrient solution to identify the most appropriate conditions . In microbioreactors with concentration gradient generators, it is possible to investigate cells in various profiles of induction factors and to determine in a single assay the ideal growth condition .…”

Section: Microfluidics: Approaches For Industrial Biotechnologymentioning

confidence: 99%

“…Differently, conventional assays such as bench scale bioreactors and shaking flasks depend on stepwise dilution of a concentrated nutrient solution to identify the most appropriate conditions. 178 In microbioreactors with concentration gradient generators, it is possible to investigate cells in various profiles of induction factors 84,112 and to determine in a single assay the ideal growth condition. 68 In addition, it allows for experimental performances in parallel, or in multiple conditions, for the same or different strains in single experiment, as presented by Hansen et al (2015).…”

Section: Microbioreactorsmentioning

confidence: 99%

Microfluidic tools toward industrial biotechnology

Oliveira

Pessoa

Bastos

et al. 2016

Biotechnology Progress

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Microfluidics is a technology that operates with small amounts of fluids and makes possible the investigation of cells, enzymes, and biomolecules and encapsulation of biocatalysts in a greater variety of conditions than permitted using conventional methods. This review discusses technological possibilities that can be applied in the field of industrial biotechnology, presenting the principal definitions and fundamental aspects of microfluidic parameters to better understand advanced approaches. Specifically, concentration gradient generators, droplet-based microfluidics, and microbioreactors are explored as useful tools that can contribute to industrial biotechnology. These tools present potential applications, inclusive as commercial platforms to optimizing in bioprocesses development as screening cells, encapsulating biocatalysts, and determining critical kinetic parameters. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1372-1389, 2016.

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High-efficiency nano/micro-reactors for protein analysis

Abstract: This article reviews the recent advances regarding the development of nanomaterial-based nanoreactors and microfluidic droplet reactors and their applications in protein analysis.

Cited by 37 publications

References 166 publications

Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors

Enzymatic Catalysis Combining the Breath Figures and Layer-by-Layer Techniques: Toward the Design of Microreactors

A coming era of precision diagnostics based on nano-assisted mass spectrometry

Microfluidic tools toward industrial biotechnology

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