High-efficiency peroxidase mimics for fluorescence detection of H₂O₂ and l-cysteine

Abstract

Enzyme-based sensing platforms have obtained rapid development in the field of diagnosis and bioanalysis. Here we present a novel fluorescent artificial enzyme-based detection strategy for L-cysteine (Cys) and H2O2 by...

2(3),9(10),16(17),23(24)-Octamethoxyphthalocyanine-Cu/CuFe2O4 nanocomposites with enhanced peroxidase-like activity for the sensitive colorimetric determination of glutathione

“…47 After adding EDTA or IPA, the absorbance value decreases to a certain extent, which means that peroxidase activity is inhibited, indicating that h + and ˙OH are generated during the catalytic process. 48 In order to further verify the catalytic mechanism of Pc(OH) 8 –Cu/CFO peroxidase mimetic enzyme, terephthalic acid (TA) was selected as a fluorescent probe. Hydroxy terephthalic acid (HTA) with fluorescence effect is generated after the reaction of TA with ˙OH, and the maximum absorption peak of HTA was produced when excited by light.…”

“…47 After adding EDTA or IPA, the absorbance value decreases to a certain extent, which means that peroxidase activity is inhibited, indicating that h + and OH are generated during the catalytic process. 48 In order to further verify the catalytic mechanism of Pc(OH) 8 -Cu/CFO peroxidase…”