High-efficient transgenic hairy roots induction in chicory: re-dawn of a traditional herb

Abstract

<p>Plant roots can be manipulated by <em>Agrobacterium rhizogenes</em> to stimulate the production of heterologous proteins for pharmaceutical applications as green cell-factories. During the present study, four bacterial strains (A4, ATCC15834, ATCC11325 and A13) in combination with three co-cultivation media (MS, B5, LS) were examined to establish an efficient and reliable transformation system for chicory (<em>Cichorium intybus</em> L.) using <em>A. rhizogenes</em>. The maximum chicory hairy roots induction was achieved using A13 strain. The observation confirmed that MS medium was more effective on hairy root growth. Dried biomass accumulation of hairy roots infected by A13 strain was 1.10 g l<sup>-1</sup> in MS medium which was significantly higher than those grown in LS and B5 medium (0.88 and 0.72 g l<sup>-1</sup>, respectively). Beta-glucuronidase (GUS) gene was introduced by A13 strain carrying the pCAMBIA1304 binary vector. The results showed that the highest frequency of transformation (63.15 %) was achieved using A13 strain and MS cultivation medium. Detection of GUS and <em>hpt</em>II genes by PCR and GUS histochemical localization confirmed the integrative transformation in hairy roots. In conclusion, the whole process was successfully optimized as a pre-step to manipulate the chicory hairy root cells to improve the unique potential of secondary metabolite production.</p>

Bacteria – Plant Interaction: Transgenic Roots for Human Health

“…High-efficient transgenic hairy root induction in chicory was demonstrated (Kabirnataj et al, 2016). Conditions of hairyroot initiation were optimized (Fathi et al, 2019).…”