2019
DOI: 10.4149/bll_2019_126
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High expression level of human epidermal growth factor (hEGF) using a well-designed fusion protein-tagged construct in E. coli

Abstract: The study was aimed at design a good fusion construct that would successfully express the recombinant proteins and produce peptides in Escherichia coli. Two different constructs including human epidermal growth factor (hEGF) gene were designed to obtain an effi cient expression level of hEGF. The hEGF sequence was inserted in pET32a vector containing thioredoxin (Trx) sequence and modifi ed pET15b vector containing intein and elastin-like polypeptide (ELP). METHODS: The vectors were transformed into E. coli TO… Show more

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