High expression of long non-coding RNA lncRNA-ATB is correlated with metastases and promotes cell migration and invasion in renal cell carcinoma

Abstract

Objective: To investigate the expression pattern of a novel long non-coding ribonucleic acid activated by transforming growth factor β, long non-coding ribonucleic acid activated by transforming growth factor β, in renal cell carcinoma tissues among the patients with various clinicopathologic features and to detect the possible role of dysregulated long non-coding RNA-ATB in renal cell carcinoma. Methods: The expression of long non-coding ribonucleic acid activated by transforming growth factor β in the renal cell carcinoma tissues and renal cancer cell lines was evaluated by quantitative real-time polymerase chain reaction. The association with clinicopathologic features was analyzed. The effects of long non-coding ribonucleic acid activated by transforming growth factor β on the renal cell carcinoma cell proliferation, apoptosis, migration, invasion and epithelial-to-mesenchymal transition were investigated by the loss-of-function approach. Results: The expression of long non-coding ribonucleic acid activated by transforming growth factor β was higher in the renal cell carcinoma tissues and renal cancer cells than in the adjacent non-tumor tissues and normal human proximal tubule epithelial cells HK-2. In addition, the long non-coding ribonucleic acid activated by transforming growth factor β expression was significantly higher in the renal cell carcinoma patients with metastasis. The elevated expression of long non-coding ribonucleic acid activated by transforming growth factor β was associated with tumor stages, histological grade, vascular invasion, lymph node metastasis and distant metastasis. Notably, we found that long non-coding ribonucleic acid activated by transforming growth factor β knockdown could (i) inhibit cell proliferation; (ii) trigger apoptosis; (iii) reduce epithelial-to-mesenchymal transition program; (iv) suppress cell migration and invasion. Conclusions: Our data have shown that long non-coding ribonucleic acid activated by transforming growth factor β actively functions as a regulator of epithelial-to-mesenchymal transition during renal cell carcinoma metastasis, which suggests that long non-coding ribonucleic acid activated by transforming growth factor β may be a potential prognostic biomarker and therapeutic target for renal cell carcinoma.

Effects of Long Non-Coding RNA LINC00963 on Renal Interstitial Fibrosis and Oxidative Stress of Rats with Chronic Renal Failure via the Foxo Signaling Pathway

“…In recent years, lncRNA has become a potential therapy in diagnosing cancer disease, including renal cell carcinoma (RCC), and the biological markers in predicting the prognosis and the treatment for human disease [21]. In this study, we explored the effect of LINC00963 on RIF and OS of CRF through the FoxO signaling pathway, and found that down-regulation of LINC00963 suppressed the RIF and OS of CRF through the activation of the FoxO signaling pathway.…”

LncRNAs act as prognostic and diagnostic biomarkers in renal cell carcinoma: a systematic review and meta-analysis

“…Furthermore, RCCRT1 [23], SPRY4-IT1 [24], NBAT-1 [27], MALAT1 [25], and ATB [22] might be used as biomarkers of lymph node metastasis. Although these lncRNAs were associated with the clinicopathological features of patients with RCC; most of these lncRNAs were detected by single study; among these lncRNAs, MALAT1 was the most investigated and reported by two studies; this lncRNA was significantly associated with the tumor stage and lymph node metastasis of patients with RCC after we pooled OR and p -value.…”

Transforming growth factor‐β activated long non‐coding RNA ATB plays an important role in acute rejection of renal allografts and may impacts the postoperative pharmaceutical immunosuppression therapy

“…It is already known that lncRNAs are involved in the regulation of gene expression, can act as miRNA sponges and scaffolds, and recruit chromatin‐modifying factors to their site of action . LncRNA‐activated by TGF‐β (lncRNA‐ATB) is a long non‐coding RNA that is highly expressed innumerous human cancers, including renal cell carcinoma . A growing number of findings confirmed the existence of a widespread interaction network of competitive endogenous RNAs (ceRNAs), in which lncRNAs may exert functions via targeting miRNAs .…”

“…14 LncRNA-activated by TGF-β (lncRNA-ATB) is a long non-coding RNA that is highly expressed innumerous human cancers, 15,16 including renal cell carcinoma. 17 A growing number of findings confirmed the existence of a widespread interaction network of competitive endogenous RNAs (ceRNAs), in which lncRNAs may exert functions via targeting miRNAs. 18 Similarly, lncRNA-ATB has been reported to promote the invasionmetastasis cascade and regulate EMT in HCC via negative modulation of miR-200 family, upregulating ZEB1, ZEB2 and suppressing E-cadherin (E-cad) expression.…”