High Expression of Stem Cell Marker ALDH1 is Associated with Reduced BRCA1 in Invasive Breast Carcinomas

Differential Expression of Cancer Stem Cell Markers ALDH1 and CD133 in Various Lung Cancer Subtypes

“…The expression of ALDH1 and CD133 were immunohistochemically evaluated using the protocol described previously (26,27). In summary, TMA slides (Superfrost plus, Thermo Scientific, Germany) were deparaffinized in xylene and rehydrated in descending concentrations of alcohol followed by distilled water.…”

Application of Stem Cells in Targeted Therapy of Breast Cancer: A Systematic Review

“…Breast cancer was the first solid cancer from which CSCs were identified and isolated in combination with flowcytometry by Al-Hajj et al (AlHajj et al, 2003;Lindeman and Visvader, 2010). Several studies using the xenograft CSC assay support the CSC model in breast cancer suggesting that ESA+/CD44+/ CD24-/low (Al-Hajj et al, 2003;Wicha et al, 2006) or aldehyde dehydrogenase 1 (ALDH1)+ phenotypes may enrich for breast CSCs which this population may be associated with a poorer prognosis (Balicki, 2007;Ginestier and Wicha, 2007;Lindeman and Visvader, 2010;Madjd et al, 2012).…”

Evaluation of HER-2/neu Overexpression in Gastric Carcinoma using a Tissue Microarray

“…Overexpression of HER2 was analyzed using rabbit polyclonal ALDH1 antibody (Dako, Denmark) By immunohistochemistry method as described previously (Madjd et al, 2012). The positively charged slides created from the recipient TMA blocks were deparafinized, rehydrated and incubated for 15 minutes with 3% hydrogen peroxidase to inhibit endogenous peroxidase activity and were then heated in 0.01 mol/L citrate buffer (pH=6.0) in a microwave oven for 5 minutes at 100ºC for antigen retrieval.…”