High extracellular calcium attenuates adipogenesis in 3T3-L1 preadipocytes

Jensen, Brian; Farach-Carson, Mary C.; Kenaley, Erin; Akanbi, Kamil

doi:10.1016/j.yexcr.2004.08.030

Cited by 72 publications

(57 citation statements)

References 47 publications

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“…19,27,28 In conformity with earlier reports 5 differentiation of preadipocytes, as indicated by GPDH activity was significantly greater in Ad-36 infected 3T3-L1 cells compared to the uninfected controls. It was noteworthy that the cell differentiation in the presence of Cidofovir was similar to that in the uninfected controls.…”

Section: Effect Of Cidofovir On Adipocyte Differentiationsupporting

confidence: 91%

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Rathod

Vangipuram²,

Krishnan

et al. 2006

Int J Obes

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Objective: Human adenovirus Ad-36 causes adiposity in animal models and shows association with human obesity. Ad-36 enhances differentiation of 3T3-L1 and human preadipocytes, without cell lysis, a characteristic that may contribute to its adipogenic effect observed in vivo. Ad-2, another human adenovirus is nonadipogenic in animals and in 3T3-L1 cells and shows no correlation with human obesity. The objective of this study was to determine the adipogenic roles of viral mRNA and DNA, which may explain the differential effects of Ad-36 and Ad-2 on preadipocyte differentiation. Methods: This study determined the duration of selected Ad-36 gene expression in 3T3-L1 cells, and the effect on preadipocytes differentiation, when Ad-36 gene expression was attenuated by Cidofovir, an antiadenoviral agent. Results:The results showed that Ad-36, but not Ad-2, expresses viral mRNA. Ad-36 gene expression peaked at 2-4 days postinoculation and very low levels persisted after day 7. Despite the viral mRNA expression, Ad-36 infection of 3T3-L1 cells was abortive as indicated by a progressive decrease in viral DNA quantity. Attenuation of Ad-36 mRNA expression by Cidofovir reduced the adipogenic effect of the virus. Conclusion: In conclusion, viral mRNA expression, although transient, is a prerequisite for enhancing differentiation of preadipocytes by Ad-36. Viral DNA replication was not required for the effect. This is the first evidence for the role of gene expression of an adipogenic human virus in enhancing preadipocytes differentiation. This study provides the basis for further understanding novel regulatory modulators of preadipocytes differentiation.

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Section: Effect Of Cidofovir On Adipocyte Differentiationsupporting

confidence: 91%

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Rathod

Vangipuram²,

Krishnan

et al. 2006

Int J Obes

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“…It was reported that elevated levels of calcium suppress adipogenesis via different mechanisms [3,6,11]. Elevated calcium levels caused a significant decrease in triglyceride accumulation in adipocytes as well as decrease the expression of adipogenic genes such as PPAR-g [13].…”

Section: Discussionmentioning

confidence: 99%

“…An increase in cytosolic calcium in 3T3-L1 preadipocytes resulted in inhibition of the early phases of adipogenesis [6,9,11]. Continuous exposure to high extracellular calcium was shown to inhibit preadipocyte differentiation to adipocytes via G-protein coupled mechanism mediated by either a novel calcium sensor or receptor [3]. Multiple calcium signaling pathways are present in human preadipocytes.…”

Section: Introductionmentioning

confidence: 99%

Expression of multiple Transient Receptor Potential channel genes in murine 3T3-L1 cell lines and adipose tissue

Bishnoi

Kondepudi

Gupta

et al. 2013

Pharmacological Reports

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“…7 It has also been reported that elevated Ca 2 + is effective in directing osteogenic lineage specification of dental pulp cells. Ca 2 + concentrations from 1.8 to 16.2 mM were analyzed, and it was found that higher concentrations increased matrix mineralization, without a significant impact on cell proliferation.…”

mentioning

confidence: 97%

Extracellular Calcium Modulates Chondrogenic and Osteogenic Differentiation of Human Adipose-Derived Stem Cells: A Novel Approach for Osteochondral Tissue Engineering Using a Single Stem Cell Source

Mellor

Mohiti-Asli

Williams

et al. 2015

Tissue Engineering Part A

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We have previously shown that elevating extracellular calcium from a concentration of 1.8 to 8 mM accelerates and increases human adipose-derived stem cell (hASC) osteogenic differentiation and cell-mediated calcium accretion, even in the absence of any other soluble osteogenic factors in the culture medium. However, the effects of elevated calcium on hASC chondrogenic differentiation have not been reported. The goal of this study was to determine the effects of varied calcium concentrations on chondrogenic differentiation of hASC. We hypothesized that exposure to elevated extracellular calcium (8 mM concentration) in a chondrogenic differentiation medium (CDM) would inhibit chondrogenesis of hASC when compared to basal calcium (1.8 mM concentration) controls. We further hypothesized that a full osteochondral construct could be engineered by controlling local release of calcium to induce site-specific chondrogenesis and osteogenesis using only hASC as the cell source. Human ASC was cultured as micromass pellets in CDM containing transforming growth factorb1 and bone morphogenetic protein 6 for 28 days at extracellular calcium concentrations of either 1.8 mM (basal) or 8 mM (elevated). Our findings indicated that elevated calcium induced osteogenesis and inhibited chondrogenesis in hASC. Based on these findings, stacked polylactic acid nanofibrous scaffolds containing either 0% or 20% tricalcium phosphate (TCP) nanoparticles were electrospun and tested for site-specific chondrogenesis and osteogenesis. Histological assays confirmed that human ASC differentiated locally to generate calcified tissue in layers containing 20% TCP, and cartilage in the layers with no TCP when cultured in CDM. This is the first study to report the effects of elevated calcium on chondrogenic differentiation of hASC, and to develop osteochondral nanofibrous scaffolds using a single cell source and controlled calcium release to induce site-specific differentiation. This approach holds great promise for osteochondral tissue engineering using a single cell source (hASC) and single scaffold.

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High extracellular calcium attenuates adipogenesis in 3T3-L1 preadipocytes

Cited by 72 publications

References 47 publications

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Viral mRNA expression but not DNA replication is required for lipogenic effect of human adenovirus Ad-36 in preadipocytes

Expression of multiple Transient Receptor Potential channel genes in murine 3T3-L1 cell lines and adipose tissue

Extracellular Calcium Modulates Chondrogenic and Osteogenic Differentiation of Human Adipose-Derived Stem Cells: A Novel Approach for Osteochondral Tissue Engineering Using a Single Stem Cell Source

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