High Flexible and Broad Antibacterial Nanodressing Induces Complete Skin Repair with Angiogenic and Follicle Regeneration

Abstract

Complete skin reconstruction is a hierarchically physiological assembly involving epidermis, dermis, vasculature, innervation, hair follicles, and sweat glands. Despite various wound dressings having been developed for skin regeneration, few works refer to the complete skin regeneration, particularly lacking for vasculatures and hair follicles. Herein, an instructive wound dressing that integrates the antibacterial property of quaternized chitin and the mechanical strength and biological multifunction of silk fibroin through layer-by-layer electrostatic self-assembly is designed and reported. The resultant dressings exhibit a nanofibrous structure ranging from 471.5 ± 212.1 to 756.9 ± 241.8 nm, suitable flexibility with tensile strength up to 4.47 ± 0.29 MPa, and broad-spectrum antibacterial activity against Escherichia coli and Staphylococcus aureus. More interestingly, the current dressing system remarkably accelerates in vivo vascular reconstruction within 15 days, and the number of regenerated hair follicles reaches up to 22 ± 4 mm −2 , which is comparable to the normal tissue (27 ± 2 mm −2). Those crucial functions might originate from the combination between quaternized chitin and silk fibroin and the hierarchical structure of electrospun nanofiber. This work establishes an easy but effective pathway to design a multifunctional wound dressing for the complete skin regeneration.

In Situ Synthesized Selenium Nanoparticles‐Decorated Bacterial Cellulose/Gelatin Hydrogel with Enhanced Antibacterial, Antioxidant, and Anti‐Inflammatory Capabilities for Facilitating Skin Wound Healing

“…The antibacterial capabilities of the hydrogels against the four bacterial strains were assessed by three methods: the inhibition test, proliferation assay, and the CFU method. [54,55] The antibacterial mechanisms were investigated by detecting the bacterial cell membrane integrity and the total ROS level in bacterial cells. All these methods are detailed in the Supporting Information.…”

Suppressive effects of plumbagin on the growth of human bladder cancer cells via PI3K/AKT/mTOR signaling pathways and EMT

“…The BCa cells were treated with PL working solution for 24 h, and then the total RNA was harvested using a commercial HiPure RNA Kit (Magen, China). The experiment was performed in accordance with manufacturers’ protocols [ 25 ], and then the concentration of obtained RNA was detected using a nanodrop spectrophotometer (Thermo, USA). After that, 1 g of RNA was applied for the reverse transcription reaction, and the resultant cDNA were further applied for quantitative real-time PCR (qRT-PCR) analysis.…”

Biocompatible and antibacterial soy protein isolate/quaternized chitosan composite sponges for acute upper gastrointestinal hemostasis

“…A compression test was carried out to evaluate the mechanical properties of SQS- n . Compressive curves and compressive strength were chosen as the key indicators of mechanical properties [ 38 ]. As shown in Fig.…”