High frequency direct shoot regeneration from Kazakh commercial potato cultivars

Abeuova, Laura S.; Kali, Balnur R; Rakhimzhanova, A. O.; Беккужина, С. С.; Manabayeva, Shuga A.

doi:10.7717/peerj.9447

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…In this experiment, a potato sprout planting method was used to test four potato varieties, and it was found that different bud locations and bud length treatments could significantly improve potato growth, quality, and yield-related indexes. This method greatly improves the reproductive coefficient of potatoes, which is consistent with previous research results on improving the utilization efficiency of potato seeds through repeated seedling breakage and transplantation [17,18].…”

Section: Discussionsupporting

confidence: 90%

Study on Potato Bud Cultivation Techniques in a Greenhouse in Spring

Wang,

Bao,

Zhang

et al. 2023

Plants

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The species degeneration caused by traditional potato cultivation methods is becoming increasingly evident, and it is particularly important to study new potato cultivation methods. Sprout planting technology has the advantages of large reproductive capacity, fast growth speed, and simplified maintenance of cultivated crops. In this study, four disease-free potato varieties (‘Fujin’, ‘Youjin’, ‘Zhongshu 4’, and ‘Feiwuruita’) were treated with different parts (top bud, middle bud, and tail bud) and different bud lengths (10 cm, 15 cm, and 20 cm), and then potato sprout planting was carried out. A nutrient pot experiment was performed following a randomized complete block design (RCBD) with various replicates and a natural control (CK) treatment. By comprehensively measuring the emergence, chlorophyll content, net photosynthetic rate, dry matter distribution during the bulking period of blocks, and effect of growth and quality with bud direct seeding under both treatments, it was found that potato block top bud direct seeding cultivation is significantly superior to other parts. In terms of early maturity and yield statistics, the advantage of top bud cultivation in ‘zhongshu 4’ is most obvious; it reaches maturity an average of 14 days earlier, and the yield can be increased by 38.05%. Therefore, top bud direct seeding is more suitable for potato sprout planting technology. On this basis, the 20 cm and 15 cm bud length treatments of top buds were used for direct cultivation, and all the above indicators performed well. Among them, in the zhongshu 4 variety, the yields of 15 cm and 20 cm bud length treatments increased by 41.78% and 38.05%, the growth rates of commercial potatoes increased by 6% and 6.9%, respectively, and the effects were the most obvious. In conclusion, the deep research and application of potato sprouting technology has high utilization value for improving potato yield and quality and has guiding significance for greenhouse potato cultivation in early spring.

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Section: Discussionsupporting

confidence: 90%

Study on Potato Bud Cultivation Techniques in a Greenhouse in Spring

Wang,

Bao,

Zhang

et al. 2023

Plants

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“…Callus was subcultured monthly and maintained on the same medium. These results are in agreement with Abeuova et al (2020). They reported that the success in nding a protocol for the in vitro regeneration of potato plants by using leaf explants depends on a variety of factors, the most important of which are cultivars and combinations of PGRs.…”

Section: Callus Induction and In Vitro Shoot Regenerationsupporting

confidence: 90%

Transformation and Expression of Na+/H+ Antiporter Vacuolar (AgNHX1) Gene in Potato (Solanum Tuberosum L.) Plants Under Salt Stress

Soliman

Kadasa

2022

Preprint

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Salinity is the main influencing factor in the productivity of most agricultural crops. Salinity has a negative effect on potato plants and yield. Previous research work shows that the transfer of the Na+/H+ antiporter gene to field crops improves the tolerance of plants to high salt concentrations. In this study, a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelinii (AgNHX1) was transferred into potato via Agrobacterium. Leaf explants induced callus on Murashige & Skoog's (MS) medium accompanied by NAA (2.00 mg l-1) and BAP (0.25 mg l-1). The in-vitro leaf generated the callus which was transferred to the regeneration medium containing ZR (2.00 mg l-1) and NAA (0.25 mg l-1). The stable integration of the AgNHX1 gene in genetically modified potato plants was confirmed by PCR analysis. RT-PCR and northern blot hybridization indicated that AgNHX1expressed highly in transgenic potato plants. Our results indicated that the expression of AgNHX1 enhanced the salt tolerance in transgenic potato plants with good growth. This happened because, under salt stress, the accumulation of Na+ content and proline was more in shoots as compared to control. It showed that the expression of AgNHX1 activity in genetically modified potato plants was four-fold higher than in unmodified potato plants. Thus, it can be said that AgNHX1 gene expression could increase salt tolerance in potato plants.

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“…12 Similar reports of successful shoot induction from internode explants on media supplemented with BAP abound in Solanum viarum 11 , Solanum tuberosum. 13 Similar report was obtained in Monochasma savatieri. 14 In Digitalis lamarckii, 0.5mg/L and 1.0 mg/L BAP was effective for direct shoot induction from leaf explants, while the lowest concentration (0.1 mg/L) and the highest concentration (3.0 mg/L) did not induce shoot.…”

Section: Bap Influenced Direct Organogenesis In Internode Explants Of In Vitro Grown Plantlets Of Solanecio Biafraesupporting

confidence: 75%

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

2021

TJNPR

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Solanecio biafrae (Olive & Hierne) C. Jeffrey (Asteraceae) is a tropical, underutilized African indigenous vegetable, highly nutritious and medicinal. It grows as undercover and is mostly collected in tree plantations such as cocoa and kola. Its propagation is seldom carried out by vines, but its climbing habit poses a problem for farmers, which leads to the application of herbicide to control their spread. In order to ascertain its potential for genetic improvement, the protocol for direct shoot organogenesis of S. biafrae was developed. Leaf lamina, petiole, and leaf with petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP) alone and in combination with Indole butyric acid (IBA). Direct shoot organogenesis was obtained from the cut end of leaf lamina and petiole explants as well from the petiolar region of the leaf with petiole explant. The results obtained from this study show the potential of these explants of S. biafrae to serve as culture material for its micropropagation, genetic improvement via transgenesis. Hence, this regeneration protocol of S. biafrae can be coupled to its genetic transformation to improve species with shorter internode and more leaves to enhance the structure of tropical vegetation.

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High frequency direct shoot regeneration from Kazakh commercial potato cultivars

Cited by 12 publications

References 21 publications

Study on Potato Bud Cultivation Techniques in a Greenhouse in Spring

Study on Potato Bud Cultivation Techniques in a Greenhouse in Spring

Transformation and Expression of Na+/H+ Antiporter Vacuolar (AgNHX1) Gene in Potato (Solanum Tuberosum L.) Plants Under Salt Stress

In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

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