High frequency in vitro regeneration system for conservation of Barleria prionitis L., a threatened medicinal shrub

Abstract

A rapid and efficient plant propagation system through nodal and embryo cul tures was developed for conservation of threatened shrub Barleria prionitis. Nodal explants exhibited high frequency shoot proliferation on Murashige and Skoog (MS) medium supplemented with 1.0 mg l 1 6 benzylaminopurine (BA) and 0.5 mg l 1 thidiazuron (TDZ). Microshoots were best rooted on half strength MS fortified with 0.5 mg l 1 indole 3 butyric acid (IBA). Maximum conversion (63.6 %) of zygotic embryos into well rooted plantlets was achieved on half strength MS supplemented with 20 mg l 1 sucrose, devoid of any growth re gu lator S. Plantlets with high PSII photochemical efficiency (Fv /Fm ≥ 0.8) were successfully shifted to natural conditions. The overall survival rate during ac cli matization from in vitro growth to field transfer was 81 %. The developed mic ro pro pagation protocol can be successfully used for large scale multiplication and conservation this high value medicinal plant species. Р Е З Ю М ЕСони В., Кумари Р., Шварнкар П.Л. Система интенсивной ре ге не ра ции in vitro для сохранения Barleria prionitis L., редкого ле карст венного кус тар ника. Разработана быстрая и эффективная система размножения рас те ний через меристемные и эмбриональные культуры для сохранения уг ро жаемого кустарникового вида Barleria prionitis. Меристемные экс плантаты де монстрировали интенсивную пролиферацию побегов на среде Mura shi ge и Skoog (MS), дополненную 1,0 мг/л 6 бензиламинопурином (BA) и 0,5 мг/л ти ди азуроном (TDZ). Микропобеги лучше всего укоренялись на MS, обогащенной 0,5 мг/л индол 3 масляной кислотой (IBA). Максимальное пре об разо ва ние (63,6 %) зиготных эмбрионов в укоренившиеся растения бы ло достигнуто на MS, до пол ненной 20 мг/л сахарозы, лишенной ка ко го ли бо регулятора роста S. Растеньица с высокой фотохимической эф фек тив ностью PSII (Fv / Fm ≥ 0,8) были успешно перенесены в ес тест вен ную среду. Общая выживаемость при акклиматизации от роста in vitro до пе ре но са в естественную среду составляла 81 %. Разработанный про то кол мик ро размножения может быть успешно использован для круп но мас штаб но го размножения и сохранения этих высокоценных лекарственных растений.

Phytochemical, pharmacological and tissue culture studies of some important species of the genus Barleria L. (Acanthaceae) - a review

“…Half strength MS media supplemented with 2.46 µm Indole butyric acid (IBA) showed good response in rooting with good mean number and the length (87). 0.5 ppm of IBA supplemented in the half strength MS media showed best results in development of roots from the in-vitro grown micro shoots of B. prionitis (89).…”

“…BAP at 1.5 ppm along with NAA at 1 ppm concentration showed best response in the development of shoot from the shoot tip explant in B. prionitis (88). MS media supplemented with 1 ppm BAP and 0.5 ppm TDZ showed best results with respect to the development of shoots from the nodal explants of B. prionitis (89). Development of callus is important task and it helps in the establishment of cell cultures.…”

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

“…Low survival rate during hardening and acclimatization is the major constraint in the large-scale production of micropropagated plantlets [15]. The high concentration of exogenous sucrose in the nutrient medium induces photosynthetic down-regulation in plantlets raised under in vitro conditions [16,17]. Therefore, prior to transfer under ex vitro conditions, photosynthetic performance of in vitro developed plantlets should be screened.…”