High Frequency Ultrasound Tissue Characterization and Acoustic Microscopy of Intracellular Changes

Brand, Sebastian; Weiss, Eike C.; Lemor, Robert; Kolios, Michael C.

doi:10.1016/j.ultrasmedbio.2008.01.017

Cited by 53 publications

(29 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Commitment to apoptosis was marked by a large increase in attenuation. This is consistent with previous studies using HeLa 57 and MCF-7 24 cell lines, with the novel observation that these higher attenuation values are associated only with early apoptosis. It is not known what may account for this increase in attenuation.…”

Section: Discussionsupporting

confidence: 93%

Properties of cells through life and death – an acoustic microscopy investigation

et al. 2015

View full text Add to dashboard Cite

Current methods to evaluate the status of a cell are largely focused on fluorescent identification of molecular biomarkers. The invasive nature of these methods -requiring either fixation, chemical dyes, genetic alteration, or a combination of these -prevents subsequent analysis of samples. In light of this limitation, studies have considered the use of physical markers to differentiate cell stages. Acoustic microscopy is an ultrahigh frequency (>100 MHz) ultrasound technology that can be used to calculate the mechanical and physical properties of biological cells in realtime, thereby evaluating cell stage in live cells without invasive biomarker evaluation. Using acoustic microscopy, MCF-7 human breast adenocarcinoma cells within the G1, G2, and metaphase phases of the proliferative cell cycle, in addition to early and late programmed cell death, were examined. Physical properties calculated include the cell height, sound speed, acoustic impedance, cell density, adiabatic bulk modulus, and the ultrasonic attenuation. A total of 290 cells were measured, 58 from each cell phase, assessed using fluorescent and phase contrast microscopy. Cells actively progressing from G1 to metaphase were marked by a 28% decrease in attenuation, in contrast to the induction of apoptosis from G1, which was marked by a significant 81% increase in attenuation. Furthermore late apoptotic cells separated into 2 distinct groups based on ultrasound attenuation, suggesting that presently-unidentified sub-stages may exist within late apoptosis. A methodology has been implemented for the identification of cell stages without the use of chemical dyes, fixation, or genetic manipulation.

show abstract

Section: Discussionsupporting

confidence: 93%

Properties of cells through life and death – an acoustic microscopy investigation

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Usually, it is difficult, if not impossible, to evaluate consecutive changes in a living cell after some stimulation or treatment. Brand et al reported intracellular changes of the acoustic properties throughout an entire treatment time course without staining by a novel acoustic microscope (SASAM, Fraunhofer Institute for Biomedical Engineering, St. Ingbert, Germany) [11]. UIM is a powerful imaging tool for such evaluation and has potential for making a diagnosis by at the cell level with non-contact high resolution imaging.…”

Section: Discussionmentioning

confidence: 99%

Ultrasonic intensity microscopy for imaging of living cells

et al. 2009

View full text Add to dashboard Cite

“…As the backscattering signals can provide useful information about acoustical and structural properties of tissue micro-structure (Brand et al 2008;Machado et al 2006), spectral analysis was performed on these signals and the following features were extracted: the predominant and global periodicity, the pre-dominant and global orientation, the variance of the periodicity function, the variance of the orientation function and the amplitude of the Discrete Fourier Transform (FT).…”

Section: Features Extractionmentioning

confidence: 99%

“…The most common representation for the backscattering signals is the B-Scan image (Szabo 2014). This technique uses the envelope of the ultrasound echoes from a region of interest (ROI) to create gray-scale images as an intensity mapping (Brand et al 2008;Huang et al 2007a;2007b;Paunksnis et al 2007; Raitelaitien_ e and Paunksnis 2005;Tabandeh et al 2000;. However, due to the processing complexity, B-Scan images commonly used in medical diagnosis contain less information than the original signals (Brand et al 2008;Despotovic et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…This technique uses the envelope of the ultrasound echoes from a region of interest (ROI) to create gray-scale images as an intensity mapping (Brand et al 2008;Huang et al 2007a;2007b;Paunksnis et al 2007; Raitelaitien_ e and Paunksnis 2005;Tabandeh et al 2000;. However, due to the processing complexity, B-Scan images commonly used in medical diagnosis contain less information than the original signals (Brand et al 2008;Despotovic et al 2008). An alternative way to represent the backscattering signals information is by the parametric Nakagami image (Tsui 2013), which is based on the envelope statistics of the A-scan signals (Caixinha et al 2013;2014;Ho et al 2012;.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation