High glucose environment induces M1 macrophage polarization that impairs keratinocyte migration via TNF-α: An important mechanism to delay the diabetic wound healing

Huang, Shu‐Mei; Wu, Ching‐Shuang; Chiu, Min‐Hsi; Wu, Chuan-Chun; Chang, Yu‐Tang; Chen, Gwo‐Shing; Lan, Cheng‐Che E.

doi:10.1016/j.jdermsci.2019.11.004

Cited by 108 publications

(88 citation statements)

References 33 publications

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“…Lindner [21] found that AKT1 is involved in the regulation of defensor expression and an important innate immune response to bacterial clearance, which is particularly important in the early control of inflammation in chronic infectious wounds. Huang [22] designed in vivo and in vitro models. Compared with the control group, skin damage caused the upregulation of TNF-α expression in the peripheral blood of diabetic rats and a significant increase in M1 macrophage infiltration, which proved that the high-glucose environment that was caused by single nuclear cells is more prone to the polarization of M1 type macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…However, the Ly6CHi influx is reversed by the selective administration of anti-MCP-1, thus improving wound healing [28]. In vitro studies have shown [22] that a high glucose environment is conducive to the polarization of M1 type macrophages and produces a large amount of TNF-α, which is not conducive to the migration of keratinocytes and affects wound healing.…”

Section: Discussionmentioning

confidence: 99%

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Study on the Mechanism of Periplaneta americana Extract to Accelerate Wound Healing after Diabetic Anal Fistula Operation Based on Network Pharmacology

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. Using network pharmacology research methods to explore the healing mechanism of American cockroach extract to accelerate wound healing after diabetic anal fistula surgery. Method. The main chemical constituents of extracts from Periplaneta americana were collected by literature retrieval. Chemical composition and targets related to diabetic anal fistula wound could be predicted based on PubChem, Swiss Target Prediction, OMIM, and GeneCards databases, and the putative targets of Periplaneta americana extraction (PAE) for diabetic anal fistula wound were obtained by Venn diagram. These common targets were predicted using the String database for protein-protein interaction (PPI) network and then screening key genes through Cytohubba. Meanwhile, the above targets were analyzed using the DAVID database for gene ontology (GO) enrichment analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) path enrichment analyses. Results. A total of 12 chemical components of PAE were obtained by literature retrieval, and 61 therapeutic targets that may accelerate the healing of diabetic anal fistula wounds were predicted by the database. According to PPI network analysis, PAE accelerates wound healing after diabetic anal fistula surgery which may be related to proteins such as AKT1, VEGFA, EGFR, CASP3, STAT3, MAPK1, TNF, JUN, ESR1, and MMP9. GO analysis results show that targets of PAE to promote wound healing were mainly involved in biological processes such as cell proliferation, macrophage differentiation, angiogenesis, and response to hypoxia. KEGG analysis showed that the target genes were mainly concentrated in the PI3K-Akt signaling pathway, HIF-1 signaling pathway, and estrogen signaling pathway. Conclusion. Periplaneta americana extract regulates multiple targets and multiple pathways to promote wound healing after diabetic anal fistula surgery. PI3K-Akt signaling pathway, HIF-1 signaling pathway, and sex hormone signaling pathway may be key pathways in the process of Periplaneta americana extract promoting wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Study on the Mechanism of Periplaneta americana Extract to Accelerate Wound Healing after Diabetic Anal Fistula Operation Based on Network Pharmacology

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The results of RNA expression on Cd68, IL-10 and TGFβ ( Figure 9G ) also showed that A300 was much stronger than other groups, corroborating that electrospinning membranes of A300 indeed promoted macrophages transforming to M2. It has been reported that M1 could inhibit keratinocyte migration [54,55] . Furthermore, the migration ability of HOK stimulated by the conditioned medium derived from Thp1 inoculated on electrospinning membranes of A300 was the strongest ( Figure S4 ).…”

Section: Resultsmentioning

confidence: 99%

The Diameter Factor of Aligned Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization in an Immune Way

Wang

Chu

Zhao

et al. 2021

Preprint

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The re-epithelialization with appendages is a major challenge to skin injury. Given that topographical factors initially modulate cell activities, the design of biomaterials with the topography (such as pattern and diameter) offers a promising strategy for achieving optimal skin wound healing. Herein, we prepare an aligned fish collagen reinforced poly (lactide-co-glycolide) electrospinning membrane (FC/PLGA) with different fiber diameters for promoting the re-epithelialization with appendages. The A300 significantly enhanced the mechanical strength and hydrophilicity, accelerated the degradation rate, and improved the cytocompatibility (proliferation and spreading) of the FC/PLGA membrane. In particular, the A300 facilitated skin wound healing (epithelization and vascularization) with the regeneration of hair follicles (HFs). The transcriptome revealed the underlying molecular mechanism that the A300 could promote macrophages transformation to reparative phenotypes (M2), subsequently exerting anti-inflammation and promoting epidermal cell migration via secreting IL10, TGFβ and MMP12. All the results indicate the A300 to be a potential candidate for guided tissue regeneration applications.

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“…And the polarization of macrophages rapidly disappeared after D7. Zhou and his colleagues report that the M1 macrophages can impair the keratinocyte migration via TNF-α [23]. So, the increased M1 percent antagonize epithelial repair.…”

Section: Discussionmentioning

confidence: 99%

IL-17 mediated macrophage polarization increased inflammatory damage in SWI-ALI models

Zhao

Zhang

et al. 2020

Preprint

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BackgroundSeawater inhalation induced acute lung injury (SWI-ALI) is the common accident in daily naval training. To investigate the mechanism of SWI-ALI will help to improve the treatment effect. Alveolar macrophages (AM) is the majority of alveolar, also paly the key role in SWI-ALI repair. IL-17 also paly the key role in the innate immunity process.MethodIn this study, we used seawater induced the ALI in mouse model. And the lungs and serum were exacted at D1, D3, D7 and D14. The AM polarization were tested by flow cytometry. The IL-17 concentration were tested by ELISA. Then the IL-17 function were confirmed by in vitro test. The mouse alveolar epithelial cell and mouse AM were co-cultured. The test compared the wound healing effect of MAE with and without IL-17.ResultThe AM switch into M1 and IL-17A increased were found after seawater dosing. And the IL-17a supplement attenuated wound healing of alveolar epithelial cells through improve the polarization of AM were confirmed in vitro model.ConclusionThe high IL-I7 micro-environment will increased the inflammatory damage through induced macrophage polarization in acute lung injury. The IL-17 antagonists have the potential to increase clinical effect in SWI-ALI treatment.

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High glucose environment induces M1 macrophage polarization that impairs keratinocyte migration via TNF-α: An important mechanism to delay the diabetic wound healing

Cited by 108 publications

References 33 publications

Study on the Mechanism of Periplaneta americana Extract to Accelerate Wound Healing after Diabetic Anal Fistula Operation Based on Network Pharmacology

Study on the Mechanism of Periplaneta americana Extract to Accelerate Wound Healing after Diabetic Anal Fistula Operation Based on Network Pharmacology

The Diameter Factor of Aligned Membranes Facilitates Cutaneous Wound Healing by Promoting Epithelialization in an Immune Way

IL-17 mediated macrophage polarization increased inflammatory damage in SWI-ALI models

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