High irradiance can minimize the negative effect of exogenous sucrose on the photosynthetic capacity of in vitro grown coconut plantlets

Fuentes, Gabriela RamÃ­rez; Talavera, C.; Desjardins, Yves; Santamaría, Jorge M.

doi:10.1007/s10535-005-7015-6

Cited by 23 publications

(12 citation statements)

References 24 publications

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“…However, the addition of sucrose to the root medium further enhanced root and shoot growth. These observations support previous studies that showed enhancement of growth by sucrose under photomixotrophic conditions (Cournac et al 1991, Paul and Stitt 1993, Furbank et al 1997, Tichá et al 1998, Fuentes et al 2005. In those studies, the increase in biomass in response to exogenous sucrose occurred mainly under low irradiance and low CO 2 (Cournac et al 1991, Tichá et al 1998, Le et al 2001.…”

Section: Discussionsupporting

confidence: 87%

Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

Mills

2009

Biologia plant.

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To study the effect of sucrose on the sink-source relationship in in vitro-grown plants, Cistus incanus seedlings and plantlets were grown horizontally in a two-compartment Petri dish (split dish), with the root system in one compartment and the shoot in the other. Shoots and roots were exposed to different sucrose concentrations (0 -30 g dm -3 ), two irradiance levels (25 and 160 µmol m -2 s -1 ) and the presence or absence of a minimum medium containing minerals and vitamins (M medium). Root and shoot biomass of the seedlings was enhanced by an increase in irradiance when the growth medium was not supplemented with sucrose indicating the role of photosynthesis in biomass production. When sucrose was added to either organ growth was enhanced as well. In the presence of sucrose in the root compartment, sucrose applied to the shoot compartment enhanced growth of both organs under low irradiance, while under high irradiance, sucrose had no further additive effect. In the absence of sucrose in the root compartment, the enhancement of root biomass by sucrose added to the shoot compartment was lower under high irradiance than under low irradiance. The response of Cistus plantlets to sucrose and irradiance differed from that of seedlings, probably reflecting a greater susceptibility of the plantlets to sucrose feedback inhibition on photosynthesis and biomass accumulation. The decrease in root and shoot growth when M medium was added to the shoot compartment and the relatively better growth of these organs when the roots were supplied with minerals and the shoot with sucrose, indicate that growth of the two organs in our experimental set-up was regulated by opposing fluxes of C and nutrients.Additional key words: sink-source relationship, two-compartment Petri dish.

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Section: Discussionsupporting

confidence: 87%

Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

Mills

2009

Biologia plant.

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“…However, an initial high sugar content in the medium can impair the carboxylation rate of explants (Langford and Wainwright 1987;Cournac et al 1991) and have negative effects on the plants that may be related to increased plant susceptibility to feedback inhibition because of an excess of carbohydrates stored in the leaves and low sugar exporta- (Capellades et al 1991;Fuentes et al 2005a). This in turn induces the loss of photosynthetic activity (Xu et al 1994;Arigita et al 2002;Fuentes et al 2005b), the decrease in the amount of rubisco and its carboxilation efficiency (Fuentes et al 2005b) or even structural disorders (Dimassi-Theriou and Bosabalidis 1997).…”

Section: Discussionmentioning

confidence: 99%

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita

Cañal

Tamés

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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In traditional in vitro culture, the low CO 2 concentration inside the vessels restricts photosynthesis and necessitates the addition of sucrose to the culture medium as the main energy source, thus bringing about changes in the absorption of mineral elements from the culture medium. In this study, we investigated macronutrient absorption and sugar consumption in Actinidia deliciosa Chevalier Liang and Ferguson cv. Hayward (kiwi), cultured on medium supplemented with varying amounts of sucrose (0, 10, and 20 g l −1 ) under both heterotrophy and autotrophy, flushed with different concentrations of CO 2 (non-ventilation, 300, 600, and 2,000 μl l −1 ). In ventilated systems with 20 g l −1 of sucrose, sucrose absorption was less than under non-ventilation. The lowest rate of sucrose absorption was recorded when the explants were cultured on medium supplemented with 20 g l −1 of sucrose and flushed with 600 μl l −1 CO 2 . Absorption of NO 3 − , PO 4 3− , and Mg 2+ were high (maximum) at the end of the culture period (40 d) in explants flushed with 600 μl l −1 CO 2 that have been cultured 20 d in the presence of sucrose and then transferred to a sucrose-free medium. These autotrophic conditions promoted maximum plant growth in terms of both fresh and dry mass as well as the length and number of shoots and leaves. The study shows that to maintain an optimum regime of mineral nutrition for prolonged culture of kiwi in vitro, an increased amount of these three ions should be supplemented in Murashige and Skoog's medium.

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“…These two techniques applied to coconut could possibly facilitate the rapid multiplication of robust plantlets, thereby creating a platform for mass clonal propagation. However, the ex vitro acclimatization of somatic embryo-derived plantlets has yet to be refined, with present rates of success of around 50 % so far (Fuentes et al 2005a). Further improvements may come from using a photoautotrophic culture system and/or through the incorporation of fatty acids, notably lauric acid, into the plantlet maturation medium (López-Villalobos et al 2001.…”

Section: Clonal Propagation Via Somatic Embryogenesis Somatic Embryogmentioning

confidence: 99%

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

Nguyen

Bandupriya

López‐Villalobos

et al. 2015

Planta

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The present review discusses not only advances in coconut tissue culture and associated biotechnological interventions but also future research directions toward the resilience of this important palm crop. Coconut (Cocos nucifera L.) is commonly known as the 'tree of life'. Every component of the palm can be used to produce items of value and many can be converted into industrial products. Coconut cultivation faces a number of acute problems that reduce its productivity and competitiveness. These problems include various biotic and abiotic challenges as well as an unstable market for its traditional oil-based products. Around 10 million small-holder farmers cultivate coconut palms worldwide on c. 12 million hectares of land, and many more people own a few coconut palms that contribute to their livelihoods. Inefficiency in the production of seedlings for replanting remains an issue; however, tissue culture and other biotechnological interventions are expected to provide pragmatic solutions. Over the past 60 years, much research has been directed towards developing and improving protocols for (i) embryo culture; (ii) clonal propagation via somatic embryogenesis; (iii) homozygote production via anther culture; (iv) germplasm conservation via cryopreservation; and (v) genetic transformation. Recently other advances have revealed possible new ways to improve these protocols. Although effective embryo culture and cryopreservation are now possible, the limited frequency of conversion of somatic embryos to ex vitro seedlings still prevents the large-scale clonal propagation of coconut. This review illustrates how our knowledge of tissue culture and associated biotechnological interventions in coconut has so far developed. Further improvement of protocols and their application to a wider range of germplasm will continue to open up new horizons for the collection, conservation, breeding and productivity of coconut.

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High irradiance can minimize the negative effect of exogenous sucrose on the photosynthetic capacity of in vitro grown coconut plantlets

Cited by 23 publications

References 24 publications

Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Tissue culture and associated biotechnological interventions for the improvement of coconut (Cocos nucifera L.): a review

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