High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors

Han, Zengsheng; Li, Qingwang; Zhang, Zhiying; Bo, Xiaochen; Gao, Dawei; Wu, Simon; Li, Jian; Zhao, Hang; Jiang, Zhongliang; Hu, Jianhong

doi:10.1016/j.pep.2006.11.019

Cited by 38 publications

(24 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In previous experiments, the recombinant adenovirus was confirmed to transfect goat mammary gland with high efficiency [25,26]. In this study, the Ad-hAT was infused directly to the mammary gland of two goats in natural lactation through the teat.…”

Section: Adenovirus Infectionmentioning

confidence: 94%

Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model

Yang

Han

et al. 2009

J Thromb Thrombolysis

Self Cite

View full text Add to dashboard Cite

Plasma-derived antithrombin (pAT) is often used for the treatments of disseminated intravascular coagulation (DIC) patients. In this paper, the recombinant adenovirus vector encoding human antithrombin (AT) cDNA was constructed and directly infused into the mammary gland of two goats. The recombinant human antithrombin (rhAT) was purified by heparin affinity chromatography from the goat milk, and then used in the treatment of thirty lipopolysaccharide (LPS) induced DIC rats. A high expression level of rhAT up to 2.8 g/l was obtained in the milk of goats. After purification, the recovery rate and the purity of the rhAT were up to 54.7 +/- 3.2% and 96.2 +/- 2.7%, respectively. In blood of the DIC rat model treated with rhAT, the levels of antithrombin and thrombin-antithrombin (TAT) were augmented significantly; meanwhile the consumption of fibrinogen and platelet was reduced significantly, and the increase of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentration was restrained modest and non-significant. For the above DIC indexes, there were no differences between pAT and rhAT (P > 0.05). Our results demonstrated that the way we established is a pragmatic tool for large-scale production of rhAT, and the rhAT produced with this method has potential as a substitute for pAT in the therapy of DIC patients.

show abstract

Section: Adenovirus Infectionmentioning

confidence: 94%

Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model

Yang

Han

et al. 2009

J Thromb Thrombolysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, in the case of HI-like loop modification of CELO, total tolerance of CAR blocking has not been achieved. Ad5-based vectors are widely used for experimental gene therapy of human breast cancer, as well as for production of recombinant proteins in the milk of animals (12). Since expression of CAR on primary breast cells is often low (22,31), we proposed that the HIRGD-modified CELO vector might be an effective tool for gene transfer into these cells.…”

Section: Discussionmentioning

confidence: 99%

Identification of HI-Like Loop in CELO Adenovirus Fiber for Incorporation of Receptor Binding Motifs

Logunov

Zubkova

Karyagina

et al. 2007

J Virol

View full text Add to dashboard Cite

“…Lactoferrin Han et al (2007) obtained elevated expression levels of hLTF in the milk of non-transgenic goats after the infusion of adenovirus into the mammary gland. In this work, although the protein was expressed in a transient manner, when compared to the current transgenic technology, the method was shown to be one of the most economic and efficient ways of producing recombinant hLTF on a large scale.…”

Section: Growth Hormonementioning

confidence: 98%

Production of recombinant proteins in milk of transgenic and non-transgenic goats

Moura

Melo

Freitas

2011

Braz. arch. biol. technol.

View full text Add to dashboard Cite

Among all the transgenic mammalians produced so far, goats have represented an excellent model of transgenesis when considering the factors such as the market demand for protein, volume of milk produced per lactation and reproductive rate. Various recombinant proteins have been obtained from the transgenic and non-transgenic goats, and among these, human antithrombin, produced by the transgenic goats, was the first recombinant protein of animal origin to be released as a drug for the clinical use in humans. This review reports the aspects inherent to the production of recombinant proteins in the goats, from the production of the animal bioreactors up to the expression of these proteins in their milk.

show abstract

High-level expression of human lactoferrin in the milk of goats by using replication-defective adenoviral vectors

Cited by 38 publications

References 33 publications

Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model

Recombinant human antithrombin expressed in the milk of non-transgenic goats exhibits high efficiency on rat DIC model

Identification of HI-Like Loop in CELO Adenovirus Fiber for Incorporation of Receptor Binding Motifs

Production of recombinant proteins in milk of transgenic and non-transgenic goats

Contact Info

Product

Resources

About