High‐Level Expression of Recombinant IgG in the Human Cell Line PER.C6

Jones, David; Kroos, Nathalie; Anema, Regina; Montfort, Bart van; Vooys, André; Kraats, Sven van der; Helm, Esmeralda van der; Smits, Shirley; Schouten, Jan P.; Brouwer, Kirsten; Lagerwerf, Fija M.; Berkel, Patrick van; Opstelten, Dirk-Jan E.; Logtenberg, Ton; Bout, A.

doi:10.1021/bp025574h

Cited by 149 publications

(90 citation statements)

References 18 publications

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“…Cells are derived from human embryonic retina cells that have been immortalized by transfecting the E1 genes from adenovirus 5 DNA. 19 Like NS0 and CHO cells, PER.C6…”

Section: Compared To Ns0 and Cho Cells Perc6mentioning

confidence: 99%

Cell Culture Processes in Monoclonal Antibody Production

Li¹,

Shen²,

Amanullah³

2013

Pharmaceutical Sciences Encyclopedia

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This chapter outlines cell culture processes in monoclonal antibody production. Cell culture process development starts with cell line generation and selection, followed by media and culture condition optimization in small‐scale systems, including 96‐well plate, shaker flasks, and bench‐scale bioreactors, for high throughput screening purposes. Once the process conditions are defined, the process is often transferred to the pilot scale to get scale‐up information and to produce toxicology materials and later to cGMP manufacturing for production of clinical material. As development of a commercial cell culture process for production of a biological product is completed at the laboratory and pilot scales, the commercialization process begins with process characterization, scale‐up, technology transfer, and validation for the manufacturing process. The strong demand of therapeutic antibody production, relatively modest titers, and reduction of cost of goods has resulted in a trend toward large‐scale manufacturing. Today, the largest biotech companies are using several hundred to 2,000 L scale bioreactors for early clinical production and 10,000‐25,000 L stirred tank bioreactors to produce commercial products.

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“…Cells are derived from human embryonic retina cells that have been immortalized by transfecting the E1 genes from adenovirus 5 DNA. 19 Like NS0 and CHO cells, PER.C6…”

Section: Compared To Ns0 and Cho Cells Perc6mentioning

confidence: 99%

Cell Culture Processes in Monoclonal Antibody Production

Li¹,

Shen²,

Amanullah³

2013

Pharmaceutical Sciences Encyclopedia

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“…식물세포의 낮은 생산성을 향상 시키고 정확한 발현수준을 조절하기 위해서 당 고갈에 의하 여 발현되는 벼 유래의 α-amylase RAmy3D promoter와 같이 특정 조건에 의해 발현이 유도되는 inducible promoter에 대 한 연구가 진행되고 있다 [5]. 또한 고발현을 제공하는 숙주 세포나 발현 벡터를 개발하는 방법 [6], 배지를 최적화 하 는 방법 [5], 낮은 온도에서 배양하는 방법 [7] 등 세포주나 공정개발을 통한 생산성 증진 연구도 활발하다. 동물세포배 양을 이용한 재조합단백질 생산의 경우 small molecule enhancer (SME)를 첨가하여 단백질 생산량을 높이는 연구 도 다수 수행되어 생산 현장에 적용되고 있다 [8].…”

Section: 서론unclassified

Enhanced Production of hCTLA4Ig by Adding Sodium Butyrate and Sodium Pyruvate

유미희¹,

김수진²,

권준영³

et al. 2011

KSBB Journal

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1) Human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), an immunosuppressive agent, was expressed in transgenic rice cells using RAmy3D promoter and RAmy1A signal peptide for the inducible production and secretion into culture media by sugar depletion. In this study, sodium butyrate was used as a small molecular enhancer (SME) to enhance the production of hCTLA4Ig in transgenic rice cell suspension cultures. When 1 mM sodium butyrate was added in sugar-free media, relative viability was not reduced, while the productivity was improved 1.3-fold. In addition, by supplementing 87 mM sodium pyruvate as an alternative energy source during the production phase, death rate of the cells was decreased. When sodium pyruvate was not added, most cells became dead at day 6. However, by adding sodium pyruvate, 18% of viability can be maintained until day 10 and the production of hCTLA4Ig was enhanced 1.4-fold. When the combination of sodium pyruvate and sodium butyrate at optimum concentrations was added, the highest viability and hCTLA4Ig production could be obtained.

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“…The most common host cell line used for the production of therapeutics is Chinese Hamster Ovary (CHO) (Cockett et al 1990, Milbrandt et al 1983) but apart from this cell line, others that are commonly used for the production of recombinant proteins are: mouse myeloma derived (NS0) (Barnes et al 2001, Bebbington et al 1992, human embryonic kidney (HEK-293) (Baldi et al 2005), baby hamster kidney (BHK) (Wurm 2004) and more recently, the human retina derived (PerC6) (Jones et al 2003) cell lines. For the purpose of process homogeneity, reproducibility and safety, chemically defined protein free medium has become a necessity.…”

Section: Host Cell Linesmentioning

confidence: 99%

Using cell engineering and omic tools for the improvement of cell culture processes

et al. 2007

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Significant strides have been made in mammalian cell based biopharmaceutical process and cell line development over the past years. With several established mammalian host cell lines and expression systems, optimization of selection systems to reduce development times and improvement of glycosylation patterns are only some of the advances being made to improve cell culture processes. In this article, the advances pertaining to cell line development and cell engineering strategies are discussed. An overview of the cell engineering strategies to enhance cellular characteristics by genetic manipulation are illustrated, focusing on the use of genomics and proteomics tools and their application in such endeavors. Included in this review are some of the early studies using the 'omic' technique to understand cellular mechanisms of product synthesis and secretion, apoptosis, cell proliferation and the influence of the physicochemical environment. The article highlights the significance of integrating genomics and proteomics data with the vast amounts of bioprocess data for improved analysis of the biological pathways involved. Further improvements of the techniques and methodologies used are needed but ultimately, the new cell engineering strategies should provide great insight into the regulatory networks within the cell in a bioprocess environment and how to manipulate them to increase overall productivity.

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High‐Level Expression of Recombinant IgG in the Human Cell Line PER.C6

Cited by 149 publications

References 18 publications

Cell Culture Processes in Monoclonal Antibody Production

Cell Culture Processes in Monoclonal Antibody Production

Enhanced Production of hCTLA4Ig by Adding Sodium Butyrate and Sodium Pyruvate

Using cell engineering and omic tools for the improvement of cell culture processes

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