High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle

Rodrı́guez, Manuel; Rubiera, Roger; Penichet, Manuel L.; Montesinos, Raquel; Cremata, José A.; Falcón, Viviana; Sanchez, Giselle; Bringas, Ricardo; Cordovés, C. O.; Valdés, Mario; Lleonart, Ricardo; Herrera, Luís; Fuente, José de la

doi:10.1016/0168-1656(94)90106-6

Cited by 163 publications

(98 citation statements)

References 19 publications

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“…A decrease in the level of rBm86-CG antibodies was noted in immunized cattle one week after infestation, which was equivalent to that observed the week before ticks were placed on the same animals ( Figure 4). Kinetics of the antibody response between cattle vaccinated with rBm86-CG and animals immunized with Gavac TM (RODRÍGUEZ et al, 1994) and TickGard PLUS were similar.…”

Section: Seroconversion Of Cattle Vaccinated With Rbm86-cgmentioning

confidence: 69%

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Cunha

León

Pinto

et al. 2012

Rev. Bras. Parasitol. Vet.

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The southern cattle fever tick, Rhipicephalus (Boophilus) microplus, is no doubt the most economically important ectoparasite of cattle globally. The inappropriate use of chemical acaricides has driven the evolution of resistance in populations of R. (B.) microplus. Anti-tick vaccines represent a technology that can be combined with acaricides in integrated control programs to mitigate the impact of R. (B.) microplus. The recombinant form of Bm86 antigen from the Campo Grande (rBm86-CG) strain of R. (B.) microplus was produced using the Pichia pastoris expression system to test its ability to immunoprotect cattle against tick infestation. Secretion of rBm86-CG by P. pastoris through the bioprocess reported here simplified purification of the antigen. A specific humoral immune response was detected by ELISA in vaccinated cattle. Immunoblot results revealed that polyclonal antibodies from vaccinated cattle recognized a protein in larval extracts with a molecular weight corresponding to Bm86. The rBm86-CG antigen showed 31% efficacy against the Campo Grande strain of R. (B.) microplus infesting vaccinated cattle. The rBm86-CG is an antigen that could be used in a polyvalent vaccine as part of an integrated program for the control of R. (B.) microplus in the region that includes Mato Grosso do Sul.Keywords: Tick, Rhipicephalus (Boophilus) microplus, anti-tick vaccine, recombinant Bm86, Pichia pastoris. ResumoO carrapato Rhipicephalus (Boophilus) microplus é, sem dúvidas, o ectoparasito economicamente mais importante para o gado a nível mundial. A utilização inadequada de acaricidas tem impulsionado a evolução da resistência em populações de R. (B.) microplus. Vacinas contra o carrapato representam uma tecnologia que pode ser combinada com acaricidas em programas de controle integrado para diminuir o impacto de R. (B.) microplus. A forma recombinante da Bm86 da cepa Campo Grande (rBm86-CG) de R. (B.) microplus foi produzido utilizando o sistema de expressão em Pichia pastoris para testar sua capacidade de imunoproteção ao gado contra a infestação de carrapatos. A secreção de rBm86-CG em P. pastoris pelo bioprocesso, simplificou a purificação do antígeno. A resposta imune humoral específica foi detectada por ELISA em soros de bovinos vacinados. Resultados de "imunoblot" revelaram que anticorpos policlonais de bovinos vacinados reconheceram uma proteína em extratos de larvas com um peso molecular correspondente à Bm86. O antígeno rBm86-CG mostrou eficácia de 31% contra a amostra CG de R. (B.) microplus utilizada para infestar os bovinos vacinados. Pelos resultados obtidos, concluímos que a rBm86-CG é um antígeno que pode ser usado em uma vacina polivalente, como parte de um programa integrado para o controle de R. (B.) microplus no estado do Mato Grosso do Sul, Brasil.

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Section: Seroconversion Of Cattle Vaccinated With Rbm86-cgmentioning

confidence: 69%

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Cunha

León

Pinto

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…There is also a correlation between antibody titers to others antigens, such as Bm95, Subolisin (SUB), Elongation Factor 1a (EF1a), Ubiquitin (UBQ), akirin (AKR) and Trypsin Inhibitor (RmLTI), and the vaccine efficacy ANDREOTTI et al, 2012;CARREÓN et al, 2012;DE LA FUENTE et al, 1998;HAVLÍKOVÁ et al, 2013;MERINO et al, 2011;MORENO-CID et al, 2013). In some experiments, different Bm86 amounts were tested, and 100 µg was accepted as the optimal composition RODRÍGUEZ et al, 1994;WILLADSEN et al, 1989). Likewise, no study has tested different routes of vaccine administration or different adjuvants to establish the best way to present vaccine antigens to the immune system of cattle.…”

Section: Discussionmentioning

confidence: 99%

Calculation of the efficacy of vaccines against tick infestations on cattle

Cunha

Andreotti

Garcia

et al. 2013

Rev. Bras. Parasitol. Vet.

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Cattle ticks are responsible for great economic losses in cattle farming worldwide, and their main control method, chemicals, has been showing problems, whether resulting from the development of resistant strains of ticks or environmental contamination. Research studies directed toward developing vaccines against ticks are emerging. One way to evaluate those vaccines is to calculate the percentage of efficacy. The aim of this study was to analyze scientific publications archived in PubMed that used this method of assessment and discuss the main factors that may affect its calculation. Thus, 25 articles addressing this subject were selected. The percentage of efficacy was usually calculated in one of two ways, with one considering the reduced fertility of eggs and the other not. The latter method may underestimate the vaccine efficacy, and the most complete formula for calculating the efficacy reflects how much the vaccine actually affects the infestation. In our view, the use of the complete formula for calculating the percentage of efficacy is broader and more representative of the vaccine effect on the tick population.Keywords: Efficiency, ticks, vaccine, bovine. ResumoCarrapatos de bovinos são responsáveis por grandes perdas econômicas para a pecuária bovina mundial e seu principal método de controle, o químico, vem apresentando problemas, seja pelo desenvolvimento de amostras de carrapatos resistentes ou pela contaminação ambiental. Na tentativa de diminuir a utilização dos acaricidas, surgem pesquisas direcionadas ao desenvolvimento de vacinas contra carrapatos. Uma maneira de avaliar essas vacinas é pelo cálculo de percentagem de eficácia. O objetivo deste trabalho foi analisar as publicações científicas indexadas no PubMed que utilizaram este método de avaliação e discutir os principais fatores que podem interferir no seu cálculo. Dessa maneira, selecionaram-se 25 artigos que tratavam desse assunto. A percentagem de eficácia apareceu sendo calculada de duas formas, uma considerando a redução da fertilidade dos ovos e a outra não. Essa última pode subestimar a eficiência da vacina, e a fórmula de cálculo da eficácia mais completa representa o quanto da infestação a vacina realmente reduziu. Em nosso entendimento, a utilização da fórmula completa para o cálculo da percentagem de eficácia é mais abrangente e representativa do efeito da vacina na população de carrapatos.Palavras-chave: Eficiência, carrapato, vacina, bovino.

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“…Protein yields of more than 1 g/l have been described (Laroche et al, 1994;Rodriguez et al, 1994). Because there is a long history, and therefore a wealth of accumulated knowledge, regarding the growth of yeast cells in large fermentors, such as is used in the brewing of beer or the production of penicillin, kilogram quantities of a protein can easily be purified.…”

Section: Introductionmentioning

confidence: 99%

Disruption of the KEX1 gene inPichia pastoris allows expression of full-length murine and human endostatin

Boehm

Pirie-Shepherd

Trinh

et al. 1999

Yeast

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By using molecular oxygen bis(μ‐oxo)dicopper(III) complexes can be produced from CuI complexes with ligand LX (LX=p‐substituted N‐ethyl‐N‐[2‐(2‐pyridyl)ethyl]‐2‐phenylethylamine; X=OMe, Me, H, Cl, NO2) in which the benzylic position of the ligand is activated and hydroxylated by the Cu2O2 core (see reaction scheme). Detailed characterization of this new C−H bond activation reaction by the bis(μ‐oxo)dicopper(III) core reveals important information on the fundamental chemistry underlying copper monooxygenase reactivity.

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High level expression of the B. microplus Bm86 antigen in the yeast Pichia pastoris forming highly immunogenic particles for cattle

Cited by 163 publications

References 19 publications

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen

Calculation of the efficacy of vaccines against tick infestations on cattle

Disruption of the KEX1 gene inPichia pastoris allows expression of full-length murine and human endostatin

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