High-light selection produces a fast-growing Picochlorum celeri

Weissman, Joseph C.; Likhogrud, Maria; Thomas, Dylan C.; Wei, Fang; Karns, Devin A.J.; Chung, Jeffrey W.; Nielsen, Robert D.; Posewitz, Matthew C.

doi:10.1016/j.algal.2018.09.024

Cited by 50 publications

(54 citation statements)

References 16 publications

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“…These microbes possess a series of unique characteristics that make them well-suited for biotechnological applications, including year-round cultivation capacity in saline water on non-arable land, higher potential biofuel yields than terrestrial crops, and the ability to utilize CO 2 as a sole carbon source 3,4 . Rising greenhouse gas emissions from anthropogenic sources has led to a resurgent interest in exploiting these organisms for concurrent CO 2 capture and renewable biocommodity production 5,6 . However, at present, current model microalgal systems are not suitable for outdoor deployment, displaying low productivity under relevant environmental conditions (e.g., high light intensity, high temperature, seawater environments) 7 .…”

Section: Introductionmentioning

confidence: 99%

“…Since its first classification in 2004, the genus Picochlorum has been recognized for its distinct characteristics of broad thermotolerance, salinity tolerance, compact genome architecture, fast doubling time, and resilience to high light intensity 6,9–13 . An alga of the genus Picochlorum was recently shown to have the highest biomass productivity in a comparative analysis between a series of industrially relevant microalgae, underscoring this genera’s deployment potential 6 . However, to date, there are limited insights into Picochlorum halotolerance, biosynthetic capacity, biomass characterization, and genetic tractability, hindering its development as a fundamental platform and for biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

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Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

et al. 2019

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Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, and development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo. This microalga displays a rapid growth rate and high diel biomass productivity (34 g m−2 day−1), with a composition well-suited for downstream processing. P. renovo exhibits broad salinity tolerance (growth at 107.5 g L−1 salinity) and thermotolerance (growth up to 40 °C), beneficial traits for outdoor cultivation. We report complete genome sequencing and analysis, and genetic tool development suitable for expression of transgenes inserted into the nuclear or chloroplast genomes. We further evaluate mechanisms of halotolerance via comparative transcriptomics, identifying novel genes differentially regulated in response to high salinity cultivation. These findings will enable basic science inquiries into control mechanisms governing Picochlorum biology and lay the foundation for development of a microalga with industrially relevant traits as a model photobiology platform.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

et al. 2019

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“…This alga was isolated using high-irradiance and elevated-temperature selective pressures on photoautotrophic enrichments that were collected near Corpus Christi, Texas 6 . Picochlorum celeri has exceptional biomass productivities that exceed 40 g m −2 d −1 when cultured using diel conditions in laboratory photobioreactors running scripts that simulate outdoor light 6 .Henley and co-workers were the first to describe the Picochlorum genus 7 , which has become the focus of several recent studies probing their high light, high salt, and high temperature tolerances, as well as their small, compact genome architectures [8][9][10][11] . The remarkable growth rates of Picochlorum celeri in seawater media is of particular interest for biofuels and other sustainable biotechnology applications 6 .…”

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Picochlorum celeri as a model system for robust outdoor algal growth in seawater

Krishnan

Likhogrud

Cano

et al. 2021

Sci Rep

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With fast growth rates, broad halotolerance and the ability to thrive at high temperatures, algae in the genus Picochlorum are emerging as promising biomass producers. Recently, we isolated a remarkably productive strain, Picochlorum celeri, that attains > 40 g m−2 day−1 productivities using simulated outdoor light. To test outdoor productivities, Picochlorum celeri was cultivated in 820 L raceway ponds at the Arizona Center for Algae Technology and Innovation. Picochlorum celeri demonstrated the highest outdoor biomass productivities reported to date at this testbed averaging ~ 31 g m−2 day−1 over four months with a monthly (August) high of ~ 36 g m−2 day−1. Several single day productivities were > 40 g m−2 day−1. Importantly for sustainability, Picochlorum celeri achieved these productivities in saline water ranging from seawater to 50 parts per thousand sea salts, without any biocides or pond crashes, for over 143 days. Lastly, we report robust genetic engineering tools for future strain improvements.

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Growth parameter estimation and model simulation for three industrially relevant microalgae: Picochlorum, Nannochloropsis, and Neochloris

Barten

Chin-On

Vree

et al. 2022

Biotech & Bioengineering

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Multiple models have been developed in the field to simulate growth and product accumulation of microalgal cultures. These models heavily depend on the accurate estimation of growth parameters. In this paper growth parameters are presented for three industrially relevant microalgae species: Nannochloropsis sp., Neochloris oleoabundans, and Picochlorum sp. (BPE23). Dedicated growth experiments were done in photobioreactors to determine the maximal biomass yield on light and maintenance rate, while oxygen evolution experiments were performed to estimate the maximal specific growth rate. Picochlorum sp. exhibited the highest specific growth rate of 4.98 ± 0.24 day −1 and the lowest specific maintenance rate of 0.079 day −1 , whereas N. oleoabundans showed the highest biomass yield on light of 1.78 g x •mol ph −1 . The measured growth parameters were used in a simple kinetic growth model for verification. When simulating growth under light conditions as found at Bonaire (12 °N, 68°W), Picochlorum sp. displayed the highest areal biomass productivity of 32.2 g.m −2 •day −1 and photosynthetic efficiency of 2.8%. The

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High-light selection produces a fast-growing Picochlorum celeri

Cited by 50 publications

References 16 publications

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo

Picochlorum celeri as a model system for robust outdoor algal growth in seawater

Growth parameter estimation and model simulation for three industrially relevant microalgae: Picochlorum, Nannochloropsis, and Neochloris

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