High lncRNA HULC expression is associated with poor prognosis and promotes tumor progression by regulating epithelial-mesenchymal transition in prostate cancer

Abstract

IntroductionRecently, increasing evidence has shown that long non-coding RNAs (lncRNAs) play critical roles in tumor progression and development. However, the expression pattern and biological function of lncRNA HULC (highly upregulated in liver cancer) in prostate cancer (PCa) remain largely unclear.Material and methodsThe expression of lncRNA HULC in 53 paired PCa tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). The χ2 test was used to explore the association of lncRNA HULC expression with clinicopathologic features. Kaplan-Meier analysis was used to detect the association between HULC expression and overall survival of PCa patients. Furthermore, the function of HULC in cell growth and metastasis was detected in PCa cells.ResultsOur data showed that HULC expression was upregulated in PCa tissues and cell lines compared to adjacent non-tumor tissues and the normal prostate cell line RWPE-1 (p < 0.05). High HULC expression was positively associated with advanced clinicopathologic features and poor overall survival (OS) for PCa patients (p < 0.05). HULC inhibition suppressed PCa cell growth and metastasis both in vitro and in vivo (p < 0.05). Furthermore, HULC knockdown reduced N-cadherin and vimentin expression and increased E-cadherin expression in PCa cells (p < 0.05).ConclusionsOur data suggested that lncRNA HULC might play oncogenic roles in PCa progression, which provided a novel therapeutic strategy for PCa patients.

Long noncoding RNA GAS5 silencing inhibits the expression of KCNQ3 by sponging miR‐135a‐5p to prevent the progression of epilepsy

“…Furthermore, miRNAs are capable of regulating the occurrence and development of the nervous system, and some specific miRNAs can regulate the pathophysiological processes of neurological diseases. For example, miR‐183 has been reported to share a close association with the occurrence of glioma, miRNA‐129‐2‐3p and miRNA‐935 are expressed in plasma and brain tissues of intractable epilepsy, and miR‐323a‐5p is abnormally expressed in patients with refractory epilepsy caused by focal cortical dysplasia …”

“…For example, miR-183 has been reported to share a close association with the occurrence of glioma, miRNA-129-2-3p and miRNA-935 are expressed in plasma and brain tissues of intractable epilepsy, and miR-323a-5p is abnormally expressed in patients with refractory epilepsy caused by focal cortical dysplasia. [28][29][30][31] Evidence has been provided that miR-135a-5p can inhibit tumor cell proliferation and promote the apoptosis of tumor cells in many tumors, while its role in epilepsy has yet to be adequately identified. 32 Reports have suggested that miR-135a-5p confer neuronal protection in mice with Alzheimer's disease by regulating potassium channels.…”

Long noncoding RNA HULC accelerates the growth of human liver cancer stem cells by upregulating CyclinD1 through miR675-PKM2 pathway via autophagy

“…HULC has been studied in several cancers and promotes tumorigenesis [1][2][3][4]. Furthermore, HULC polymorphisms are associated with hepatocellular cancer risk and prognosis [5,6]. In addition, HULC enhances autophagy [7] and facilitates hepatocellular carcinoma genesis [8].…”

lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

“…Numerous evidences have shown that lncRNAs are involved in regulating various biological characteristics of tumors, including proliferation, apoptosis, metastasis, and metabolism [13,14]. Previous microarray results showed that a large number of lncRNAs were significantly downregulated or upregulated in different cancer tissues [15,16]. lncRNAs are also involved in the occurrence and development of RCC, such as knockdown of lncRNA ROR inhibiting the proliferation, migration, and invasion of RCC cells.…”