High mass resolution SIMS

Maharrey, Sean P.; Bastasz, R.; Behrens, Richard; Highley, Aaron M.; Hoffer, Saskia; Kruppa, Gary; Whaley, Josh A.

doi:10.1016/j.apsusc.2004.03.197

Cited by 17 publications

(12 citation statements)

References 4 publications

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“…The majority of imaging mass spectrometry instruments now use ToF analyzers, which can provide mass resolution in excess of 10,000 and mass accuracies below 20 ppm. The ultra-high mass resolution and mass accuracy of the Fourier transform ion cyclotron resonance mass spectrometer, resolution >100,000 and sub part-per-million mass accuracy, is beginning to be developed for imaging mass spectrometry experiments (Maharrey et al, 2004;Taban et al, 2007).…”

Section: A Mass Resolutionmentioning

confidence: 99%

Imaging mass spectrometry

McDonnell

Heeren

2007

Mass Spectrometry Reviews

1,013

842

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Imaging mass spectrometry combines the chemical specificity and parallel detection of mass spectrometry with microscopic imaging capabilities. The ability to simultaneously obtain images from all analytes detected, from atomic to macromolecular ions, allows the analyst to probe the chemical organization of a sample and to correlate this with physical features. The sensitivity of the ionization step, sample preparation, the spatial resolution, and the speed of the technique are all important parameters that affect the type of information obtained. Recently, significant progress has been made in each of these steps for both secondary ion mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization (MALDI) imaging of biological samples. Examples demonstrating localization of proteins in tumors, a reduction of lamellar phospholipids in the region binding two single celled organisms, and sub-cellular distributions of several biomolecules have all contributed to an increasing upsurge in interest in imaging mass spectrometry. Here we review many of the instrumental developments and methodological approaches responsible for this increased interest, compare and contrast the information provided by SIMS and MALDI imaging, and discuss future possibilities. #

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Section: A Mass Resolutionmentioning

confidence: 99%

Imaging mass spectrometry

McDonnell

Heeren

2007

Mass Spectrometry Reviews

1,013

842

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“…Concerning the high mass accuracy, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) is used in combination with SIMS capabilities. [102][103][104][105][106][107] Even if they are expensive and located in few laboratories in the world, such instruments were able to provide mass resolutions above 3 000 000 (m/Dm 50% ) that enable the unambiguous molecular identication of ions based on the mono-isotopic peak and the isotopic prole in combination with high lateral SIMS resolution.…”

Section: Molecular Identificationmentioning

confidence: 99%

A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Massonnet

Heeren

2019

J. Anal. At. Spectrom.

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“…Advances in instrumentation have resulted in innovative TOF-SIMS instruments with MS/MS capabilities that are now employed to address this limitation, in particular for biological applications. Examples include the implementation of a gallium liquid metal ion gun (LMIG) on a Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometer for high-resolution imaging combined with high mass resolution and MS/MS to achieve accurate identification [ 15 ], a C 60 cluster ion source that was combined with FT-ICR to study larger biomolecules [ 16 ] along with several other tandem MS approaches [ 13 , 17 , 18 ]. The most recent development in this field is the 3D OrbiSIMS, presented by Passarelli and co-workers [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry

Bruinen

Fisher

Balez

et al. 2018

J. Am. Soc. Mass Spectrom.

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A unique method for identification of biomolecular components in different biological specimens, while preserving the capability for high speed 2D and 3D molecular imaging, is employed to investigate cellular response to oxidative stress. The employed method enables observing the distribution of the antioxidant α-tocopherol and other molecules in cellular structures via time-of-flight secondary ion mass spectrometry (TOF-SIMS (MS1)) imaging in parallel with tandem mass spectrometry (MS2) imaging, collected simultaneously. The described method is employed to examine a network formed by neuronal cells differentiated from human induced pluripotent stem cells (iPSCs), a model for investigating human neurons in vitro. The antioxidant α-tocopherol is identified in situ within different cellular layers utilizing a 3D TOF-SIMS tandem MS imaging analysis. As oxidative stress also plays an important role in mediating inflammation, the study was expanded to whole body tissue sections of M. marinum-infected zebrafish, a model organism for tuberculosis. The TOF-SIMS tandem MS imaging results reveal an increased presence of α-tocopherol in response to the pathogen. Graphical Abstractᅟ Electronic supplementary materialThe online version of this article (10.1007/s13361-018-1979-x) contains supplementary material, which is available to authorized users.

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High mass resolution SIMS

Cited by 17 publications

References 4 publications

Imaging mass spectrometry

Imaging mass spectrometry

A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Identification and High-Resolution Imaging of α-Tocopherol from Human Cells to Whole Animals by TOF-SIMS Tandem Mass Spectrometry

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