High Micronucleus Frequency in Peripheral Blood Lymphocytes of Untreated Cancer Patients Irrespective of Gender, Smoking and Cancer Sites

Milošević-Djordjević, Olivera; Grujičić, Darko; Vaskovic, Zeljko; Marinković, Darko

doi:10.1620/tjem.220.115

Cited by 37 publications

(22 citation statements)

References 33 publications

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“…This result is in agreement with the previously published conclusions that the baseline MN frequency is higher in thyroid cancer patients (Iarmarcovai et al 2008) and in patients with other types of tumours (BaciuchkaPalmaro et al 2002;Bonassi et al 2007;Murgia et al 2008;Milošević-Djordjević et al 2010) than in healthy persons. Similarly, the increase in the mean MN frequency in PBLs of patients after 131-I therapy is consistent with the previous publications (Ballardin et al 2002;Popova et al 2005).…”

Section: Discussionsupporting

confidence: 93%

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Vrndic

Milošević-Djordjević

Mijatovic-Teodorovic

et al. 2013

Tohoku J. Exp. Med.

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Differentiated thyroid cancers (DTCs) derive from thyroid follicular cells and include papillary and follicular cancers. In patients with DTCs, the initial treatment includes thyroidectomy and radioactive iodine (131-I) therapy. The objective of this study was to examine whether the intensity of DNA damage in peripheral blood lymphocytes (PBLs) of DTC patients depends on the amount of 131-I retained in the selected regions of interest (thyroid and abdominal region) as well as in the whole-body 72 hours after therapy. In addition, the possible influence of other factors that may affect micronuclei (MN) frequency, such as age, gender, smoking habits, and histological type of tumour was analyzed. The study population consisted of 22 DTC patients and 20 healthy donors. Data on the distribution of 131-I were obtained from the whole-body scans. MN frequency and cytokinesis-block proliferation index (CBPI) were measured using cytokinesis-block micronucleus assay. 131-I therapy significantly increased the MN frequency (19.50 ± 6.90 vs. 27.10 ± 19.50 MN) and significantly decreased the CBPI (1.52 ± 0.20 vs. 1.38 ± 0.17) in patients' lymphocytes. There was a clear correlation between the increased MN frequency and 131-I accumulation in the thyroid region in patients without metastases. The MN values did not differ in relation to the factors that could affect MN, such as age, gender, smoking habits, and histological type of tumour. In conclusion, the MN frequency in PBLs of DTC patients without metastases depends on the accumulation of 131-I in the thyroid region and does not depend on the other factors examined.

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Section: Discussionsupporting

confidence: 93%

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Vrndic

Milošević-Djordjević

Mijatovic-Teodorovic

et al. 2013

Tohoku J. Exp. Med.

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“…Many authors have published data on the effects of age on MN frequency in human peripheral blood lymphocytes, but the results are conflicting [3,[9][10][11][12][13][14][15][16][17][18][19][20]27,30]. However, a clear correlation of increasing MN frequency with advancing age was demonstrated in most studies [3,[9][10][11][12][13][14][15][16][17][18][19]30].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, some studies have provided strong evidence that the MN frequency in the peripheral blood lymphocytes is a predictor of cancer risk, and that the increase in MN frequency is associated with early events of carcinogenesis [23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genotoxicity and cytotoxicity in the general population in Turkey by use of the cytokinesis-block micronucleus cytome assay

Dönmez-Altuntaş

Bıtgen

2012

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Different human health problems are associated with chromosomal instability in lymphocytes (22)(23)(24). Today, cytogenetics provides numerous different biomarkers for evaluating chromosomal instability and scoring of MN in lymphocytes is one that draws a lot of interest.…”

Section: Discussionmentioning

confidence: 99%

Chromosomal instability in peripheral blood lymphocytes of patients with reproductive failure assessed by micronucleus assay

Milošević-Djordjević

Stošić

Grujičić

et al. 2012

Archives of Industrial Hygiene and Toxicology

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We investigated chromosomal instability in peripheral blood lymphocytes (PBL) of patients with reproductive failure in respect to age, smoking habits, gender, miscarriages, and semen parameters. The study involved 36 individual cases of reproductive failure (18 men and 18 women) attended at the Clinical Centre of Kragujevac, Serbia, and 30 healthy subjects (15 men and 15 women). Micronuclei (MN) frequency was estimated in PBL using the cytokinesis-block micronucleus (CBMN) assay. The baseline MN frequencies were signifi cantly higher (p=0.031; p<0.001) in male [(9.22 ± 4.70) MN per 1000 BN cells] and female patients [(13.50 ± 2.5) MN per 1000 BN cells] than in male and female healthy controls [(6.27 ± 2.66) MN per 1000 BN cells; (6.80 ± 2.98) MN per 1000 BN cells]. The mean baseline MN frequency did not signifi cantly differ between miscarriage groups and between patients with and without normal values of semen parameters. The correlations between poor sperm concentration (<20x10 6 mL -1 ), rapid progressive motility (<25 %), normal morphology (<30 %), and MN frequencies were negative, but not statistically signifi cant. We found that only gender signifi cantly infl uenced the MN rates in analysed patients. There were no signifi cant differences between age groups and between smokers and non-smokers in patients and control samples. We conclude that the increase in baseline MN frequency in PBL of patients with reproductive failure corresponds to the increase in chromosomal damage, which occurs as a result of complex events that cause reproductive disorders.

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High Micronucleus Frequency in Peripheral Blood Lymphocytes of Untreated Cancer Patients Irrespective of Gender, Smoking and Cancer Sites

Abstract: Keywords: chromosomal instability/micronuclei/peripheral blood lymphocytes/untreated cancer patients/different cancer site Tohoku J. Exp. Med., 2010, 220 (2), 115-120.

Cited by 37 publications

References 33 publications

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

Evaluation of the genotoxicity and cytotoxicity in the general population in Turkey by use of the cytokinesis-block micronucleus cytome assay

Chromosomal instability in peripheral blood lymphocytes of patients with reproductive failure assessed by micronucleus assay

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