High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival

Goggs, Robert; Letendre, Jo‐Annie

doi:10.3389/fvets.2018.00179

Cited by 13 publications

(17 citation statements)

References 71 publications

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“…A previously validated kit (Biomedica Gruppe, Vienna, Austria), was used to measure NT-proCNP concentrations (57, 62). An antibody-coated microsphere-based multiplex cytokine immunoassay kit (CCYTOMAG-90K, EMD-Millipore, Billerica, MA), designed for the simultaneous quantification of multiple cytokines was used as previously described (64, 71). This assay was performed with overnight sample incubation at 4°C to maximize sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Biomarker Guided Diagnosis of Septic Peritonitis in Dogs

Martiny

Goggs

2019

Front. Vet. Sci.

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Septic peritonitis (SP) is common in dogs and is associated with high mortality. Early recognition is essential to maximizing survival and may be aided by biomarker measurement. The present study aimed to evaluate the ability of biomarkers to discriminate septic peritonitis from non-septic ascites (NSA). Eighteen dogs with SP and 19 age-matched controls with NSA were enrolled. Contemporaneous blood and peritoneal effusion samples were obtained. Concentrations of cell-free DNA (cfDNA), cytokines, glucose, lactate, N-terminal pro-C-type natriuretic peptide (NT-proCNP), nucleosomes, and procalcitonin (PCT) were measured using commercial reagents and assays. Paired biomarker concentrations were compared with the Wilcoxon matched-pairs signed rank test, and biomarker concentrations between groups were compared with the Mann-Whitney U -test. P -values were adjusted for multiple comparisons using the Bonferroni correction. Receiver operating characteristic curves were generated to assess the ability of the above biomarkers to discriminate SP from NSA. Dogs with SP had significantly greater blood CCL2 concentrations than dogs with NSA ( P = 0.032). Dogs with SP had significantly greater effusion CCL2, IL-6, IL-10, and lactate concentrations than dogs with NSA ( P ≤ 0.0121). Blood-effusion concentration gradients of CCL2, glucose, IL-6, IL-10, and lactate were significantly different in dogs with SP compared to dogs with NSA ( P ≤ 0.0165). Effusion lactate concentration had the highest AUROC value (0.866, 95% CI 0.751–0.980, P = 0.0001), although other biomarkers performed similarly. An effusion lactate concentration of 4.2 mmol/L was 72.2% (95% CI 46.5–90.3%) sensitive and 84.2% (95% CI 60.4–96.6%) specific for the diagnosis of SP.

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Section: Methodsmentioning

confidence: 99%

Biomarker Guided Diagnosis of Septic Peritonitis in Dogs

Martiny

Goggs

2019

Front. Vet. Sci.

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“…It is responsible for neutrophil recruitment, margination, and tissue infiltration. IL-8 has received attention as a marker of sepsis and bacteremia in people (26,27,34) and it may be a useful marker in dogs with polyarthritis, sepsis and trauma (13,35,36). Its specificity for sepsis is not known in cats, but increased IL-8 concentrations have been documented in the course of sterile inflammatory diseases like feline idiopathic cystitis and degenerative joint disease (20,22).…”

Section: Discussionmentioning

confidence: 99%

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

et al. 2020

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Background: Sepsis is a life-threatening condition associated with an exacerbated production of both pro-and anti-inflammatory cytokines that can promote a hyperactive response to infection or induce immunoparalysis. Data regarding the immune response to sepsis in cats are scarce. Establishing the profiles of cytokines and chemokines in feline sepsis to characterize the nature of the immune responses to sepsis might enable individualized treatments to be developed and targeted. Objective: To evaluate the cytokine and chemokine network in cats with sepsis and septic shock, and to investigate the associations of these analytes with disease severity and outcome. Methods: Blood samples prospectively collected at presentation of cats with sepsis and septic shock to two veterinary teaching hospitals were analyzed. Forty healthy cats were included as controls. A 19-plex feline cytokine/chemokine magnetic bead assay system was used to measure analytes in citrated plasma samples. Cytokine concentrations were compared between groups using the Kruskal-Wallis test with Dunn's post-hoc correction for multiple comparisons. Cytokine concentrations were compared between survivors and non-survivors with the Mann-Whitney U test. Odds ratios were calculated using logistic regression. A multivariable logistic regression model for prediction of septic shock was constructed. Results: The study enrolled 35 septic cats. Many cytokines were undetectable in both sick and healthy control cats and were excluded from subsequent analyses. Comparisons of cytokine concentrations among healthy controls, cats with sepsis (n = 12) and cats with septic shock (n = 23) revealed that sick cats (sepsis or septic shock) had significantly higher plasma concentrations of IL-6, IL-8, KC-like, and RANTES compared to healthy controls. The combination of MCP-1, Flt-3L, and IL-12 was predictive of septic shock. None of the cytokines analyzed was predictive of outcome in this study population. Conclusion: Plasma concentrations of IL-6, IL-8, KC-like, and RANTES are increased in cats with sepsis and may play important roles in pathogenesis. Multivariable modeling suggested that analysis of cytokines might aid differentiation of septic shock from sepsis. None of the cytokines analyzed was predictive of outcome. Measurement of these cytokines might enable future studies to better diagnose and characterize feline sepsis and septic shock.

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“…This second plate-based immunoassay, the Canine ProInflammatory Panel 3 Ultra-Sensitive Kit (Meso Scale Diagnostics (MSD), Rockville, Maryland, USA) measures four cytokines (IL-2, IL-6, CXCL8, TNF-α) using a proprietary electrochemiluminescent detection system, QuickPlex SQ 120 (MSD, Rockville, Maryland, USA) and has also been used by various groups to measure cytokine concentrations in canine serum and plasma samples. 30 33–35 For both assays, standard curves were generated in duplicate using manufacturer supplied reagents. All sample analyses were performed in duplicate and the mean values of duplicate readings used for statistical analyses.…”

Section: Methodsmentioning

confidence: 99%

“…Another immunoassay with overlapping spectra but with a distinct assay principle was used to augment cytokine detection. This second plate‐based immunoassay, the Canine ProInflammatory Panel 3 Ultra‐Sensitive Kit (Meso Scale Diagnostics (MSD), Rockville, Maryland, USA) measures four cytokines (IL‐2, IL‐6, CXCL8, TNF‐α) using a proprietary electrochemiluminescent detection system, QuickPlex SQ 120 (MSD, Rockville, Maryland, USA) and has also been used by various groups to measure cytokine concentrations in canine serum and plasma samples 30 33–35. For both assays, standard curves were generated in duplicate using manufacturer supplied reagents.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of cytokine concentrations in a trehalose‐stabilised lyophilised canine platelet product: a preliminary study

Goggs

Cremer²,

Brooks

2020

Veterinary Record Open

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BackgroundPlatelet transfusion is indicated for haemorrhage due to severe thrombocytopenia and for trauma associated coagulopathy. Febrile non-haemolytic transfusion reactions are a common complication of platelet transfusions in people and may be due to accumulated inflammatory cytokines. The present study aimed to determine the cytokine profile of a novel canine lyophilised platelet product following reconstitution, to assess the lyophilised platelets’ activation response to physiological platelet agonists and to compare the cytokine profiles of basal and stimulated canine lyophilised platelets.MethodsCell counts and biochemical analyses were conducted following reconstitution. Cytokine concentrations were measured with a canine-specific multiplex immunocapture assay and with an electrochemiluminescent ELISA. Aliquots of reconstituted product from three separate vials were activated for 10 minutes under non-stirred conditions using adenosine diphosphate, thrombin or convulxin and their cytokine concentrations compared with unactivated samples. Flow cytometry and light-transmission aggregometry were used to evaluate the product’s ability to express a procoagulant surface, degranulate and aggregate. Fresh platelet-rich plasma was used as a positive control.ResultsThe product had a mean±SD particle count of 1.23±0.2×109/ml, contained platelets that expressed surface phosphatidylserine before agonist stimulation and was capable of aggregation in response to thrombin stimulation suggesting that the product may have haemostatic potential following in vivo administration. Cytokine concentrations measured by the immunocapture assay were generally low, while twofold to threefold increases relative to published intervals were noted for several cytokines using the ELISA. Concentrations of chemokine (C-X-C) motif ligand 8 and tumour necrosis factor-α were significantly increased as measured by the ELISA, but not by the immunocapture assay, while concentrations of KC-like were significantly increased as measured by the immunocapture assay. Stimulation with platelet agonists did not affect measured cytokine concentrations.ConclusionFurther study of the effects of administration of this lyophilised platelet product is warranted.

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High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival

Cited by 13 publications

References 71 publications

Biomarker Guided Diagnosis of Septic Peritonitis in Dogs

Biomarker Guided Diagnosis of Septic Peritonitis in Dogs

Cytokine and Chemokine Profiling in Cats With Sepsis and Septic Shock

Evaluation of cytokine concentrations in a trehalose‐stabilised lyophilised canine platelet product: a preliminary study

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