High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis

Musisi, Emmanuel; Sessolo, Abdul; Kaswabuli, Sylvia; Zawedde, Josephine; Byanyima, Patrick; Kasinga, Shariifah; Sanyu, Ingvar; Uwimaana, Esther; Walimbwa, Stanley; Olore, Joseph; Ssengooba, Willy; Sekaggya, Christine; Joloba, Moses; Worodria, William; Huang, Laurence; Gillespie, Stephen H.; Sloan, Derek J.; Sabiiti, Wilber

doi:10.1128/spectrum.02100-21

Cited by 10 publications

(16 citation statements)

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“…The need for an alternative sample type to diagnose TB cannot be more strongly emphasized. We have shown that viable M. tuberculosis bacilli are quantifiable by the TB molecular bacterial load assay (TB-MBLA) in stool samples processed using OMNIgene-Sputum (OM-S) medium ( 4 ).…”

Section: Lettermentioning

confidence: 99%

“…A set of stool samples corresponding to those processed in the OM-S study were processed using PBS and stored at −20°C until RNA extraction was performed ( 4 ). Six grams of stool per patient was processed within 20 min after collection, prior to storage.…”

Section: Lettermentioning

confidence: 99%

“…We acknowledge all contributors to the paper ( 4 ) that is sister to the data set presented in this letter.…”

Section: Acknowledgmentsmentioning

confidence: 99%

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Detection and Quantification of Viable Mycobacterium tuberculosis Bacilli in Saline-Processed Stool Samples by Tuberculosis Molecular Bacterial Load Assay: a Potential Alternative for Processing Stool

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Section: Lettermentioning

confidence: 99%

Section: Lettermentioning

confidence: 99%

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Detection and Quantification of Viable Mycobacterium tuberculosis Bacilli in Saline-Processed Stool Samples by Tuberculosis Molecular Bacterial Load Assay: a Potential Alternative for Processing Stool

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“…DNA-based Xpert MTB/RIF assays are very sensitive, but their positivity does not necessarily mean viable TB bacilli [ 4 , 5 ]. Recent reports demonstrate the promising performance of the Xpert MTB/RIF Ultra in pediatric stools and cerebrospinal fluid [ 6 ], which will improve the detection of TB in this group of patients and those who cannot produce sputum.…”

Section: Introductionmentioning

confidence: 99%

Performance of Tuberculosis Molecular Bacterial Load Assay Compared to Alere TB-LAM in Urine of Pulmonary Tuberculosis Patients with HIV Co-Infections

Mapamba

Sauli

Lalashowi

et al. 2023

IJMS

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Alternative tools are needed to improve the detection of M. tuberculosis (M. tb) in HIV co-infections. We evaluated the utility of Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) compared to lipoarabinomannan (LAM) to detect M. tb in urine. Sputum Xpert MTB/RIF-positive patients were consented to provide urine at baseline, weeks 2, 8, 16, and 24 of treatment for TB-MBLA, culture, and LAM. Results were compared with sputum cultures and microscopy. Initial M. tb. H37Rv spiking experiments were performed to validate the tests. A total of 63 urine samples from 47 patients were analyzed. The median age (IQR) was 38 (30–41) years; 25 (53.2%) were male, 3 (6.5%) had urine for all visits, 45 (95.7%) were HIV positive, of whom 18 (40%) had CD4 cell counts below 200 cells/µL, and 33 (73.3%) were on ART at enrollment. Overall urine LAM positivity was 14.3% compared to 4.8% with TB-MBLA. Culture and microscopy of their sputum counterparts were positive in 20.6% and 12.7% of patients, respectively. Of the three patients with urine and sputum at baseline, one (33.33%) had urine TB-MBLA and LAM positive compared to 100% with sputum MGIT culture positive. Spearman’s rank correction coefficient (r) between TB-MBLA and MGIT was −0.85 and 0.89 with a solid culture, p > 0.05. TB-MBLA has the promising potential to improve M. tb detection in urine of HIV-co-infected patients and complement current TB diagnostics.

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“…Recent reports have demonstrated that the Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) VitalBacteria ™ has potential to replace culture on monitoring bacterial load during TB treatment (7)(8)(9). The 2018 global tuberculosis report acknowledged TB-MBLA as a candidate to replace smear microscopy and culture for monitoring treatment response (10).…”

Section: Introductionmentioning

confidence: 99%

Reduction of blood C-reactive protein concentration complements the resolution of sputum bacillary load in patients on anti-tuberculosis therapy

et al. 2022

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Background: Tuberculosis (TB) is a difficult-to-treat disease requiring the combination of four antibiotics for a minimum of 6 months. Rapid and quantitative biomarkers to monitor treatment response are urgently needed for individual patient management and clinical trials. C-reactive protein (CRP) is often used clinically as a rapid marker of inflammation caused by infection. We assessed the relationship of TB bacillary load and CRP as biomarkers of treatment response.Methods: Xpert MTB/RIF-confirmed pulmonary TB cases were enrolled for treatment response assessment in Mozambique. Treatment response was measured using the Tuberculosis Molecular Bacterial Load Assay (TB-MBLA) in comparison with standard-of-care Mycobacterium Growth Indicator Tube (MGIT) culture at baseline and at weeks 1, 2, 4, 8, 12, 17, and 26 of treatment. Blood CRP concentration was measured at baseline, week 8, and week 26. Treatment response was defined as increase in MGIT culture time to positivity (TTP), and reduction in TB-MBLA-measured bacillary load and blood CRP concentration.Results: Out of the 81 screened presumptive TB cases, 69 were enrolled for 6month treatment follow-up resulting in 94% treatment completion rate. Four participants did not complete TB treatment and 22 participants had missing CRP or TB-MBLA results and were excluded from TB-MBLA-CRP analysis. The remaining 43 participants-median age, 31 years old [interquartile range (IQR): 18-56]; 70% (30/43) male; and 70% (30/43) infected with HIV-were considered for analysis. Culture TTP and bacillary load were inversely Frontiers in Immunology frontiersin.org 01

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High Mycobacterium tuberculosis Bacillary Loads Detected by Tuberculosis Molecular Bacterial Load Assay in Patient Stool: a Potential Alternative for Nonsputum Diagnosis and Treatment Response Monitoring of Tuberculosis

Cited by 10 publications

References 50 publications

Detection and Quantification of Viable Mycobacterium tuberculosis Bacilli in Saline-Processed Stool Samples by Tuberculosis Molecular Bacterial Load Assay: a Potential Alternative for Processing Stool

Detection and Quantification of Viable Mycobacterium tuberculosis Bacilli in Saline-Processed Stool Samples by Tuberculosis Molecular Bacterial Load Assay: a Potential Alternative for Processing Stool

Performance of Tuberculosis Molecular Bacterial Load Assay Compared to Alere TB-LAM in Urine of Pulmonary Tuberculosis Patients with HIV Co-Infections

Reduction of blood C-reactive protein concentration complements the resolution of sputum bacillary load in patients on anti-tuberculosis therapy

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