High Performance Liquid Chromato-Graphic Assay For 5-Fluorouracil and 5-Fluorocytosine

Biondi, Luciano; Nairn, J. Graham

doi:10.1080/01483918508074102

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…Tomasi et al was used. The resulting values are shown in the Tables I and II.According to table II computationally obtained pK a1 value (lost from N 1 ) is very close to the experimental pK a1 value and for pK a2 (lost from N 2 ) calculated value is relatively : from Ref[24] 24 …”

supporting

confidence: 71%

The Prediction of Nanoscale Drug Molecular Structure and Acid Dissociation Constants of 5-Fluorouracil in Aqueous Solution Using DFT Methods

Koohyar¹,

Kiani²,

Abbaszadeh³

et al. 2013

IJFAS

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Background and Objective : In this work, dissociation of nano drug 5-Fluorouracil derivatives was studied theoretically.Methodology : For this purpose, Gibbs free energy values for neutral and deprotonated forms of 5-Fluorouracil were calculated atgas and aqueous phases by using density functional theory (DFT) method. Solvent effects are taken into account by means ofpolarizable continuum model (PCM). Result : It was shown that, theoretically calculated pKa values are in good agreement withthe existing experimental pKa values, which are determined from capillary electrophoresis, potentiometric titration and UV–visiblespectrophotometric measurements. Conclusion : In summary, cluster continuum method with implicit-explicit solvent moleculeswas used for calculation of pKa values. Total energies and molecular parameters were obtained for 5-FUra nanoscale drug systems,at B3LYP/6-31G(d) level of theory for the anion, cation, and neutral species.

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supporting

confidence: 71%

The Prediction of Nanoscale Drug Molecular Structure and Acid Dissociation Constants of 5-Fluorouracil in Aqueous Solution Using DFT Methods

Koohyar¹,

Kiani²,

Abbaszadeh³

et al. 2013

IJFAS

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“…The retention times can be increased and the peak shapes improved using ion-pair reactants. In the analysis of fluoropyrimidines, it was suggested to use the following systems: at low pH of the mobile phase (below 4.0), sodium dodecylsulfate [96], heptylsulfonic acid [53,97], hexylsulfonic acid [98], and pentylsulfonic acid [97]; at higher pH (above 4.0), tetrabutylammonium (TBA) hydroxide [99,100], TBA phosphate [101,102], tetraethylammonium (TEA) chloride [103], TEA bromide and hydrosulfate [100], and hexadecyltrimethylammonium bromide [104,105]. Idoxuridine (2¢-deoxy-5-iodouridine) and trifluridine (2¢-deoxy-5-trifluoromethyluridine).…”

Section: Uracil and Uridine Derivativesmentioning

confidence: 99%

“…The blood serum was pretreated with sulfuric acid and sodium tungstate [150]. In most cases, the analyses were performed using ion-pair reactants such as sodium pentanesulfonate [108,145], heptanesulfonate [145,147], and octanesulfonate [141,144], TBA bromide [150], and TEA chloride [143].…”

Section: Cytarabine (1-[b-d-arabinofuranosyl]cytosine) Fluorocytosinmentioning

confidence: 99%

HPLC in biopharmaceutical investigations of drugs representing pyrimidine derivatives (A review)

et al. 2007

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Pyrimidine derivatives are widely used for the treatment of viral diseases and cancer. The analysis of pyrimidine derivatives is typically performed using various chromatographic techniques, in particular, reversed-phase high performance liquid chromatography (HPLC). The separation is typically carried out with (7 -30)-cm-long C 8 and C 18 silica gel columns, mainly at room temperature, and a 1 -1.5 ml/min eluent flow rate. The column is eluted in an isocratic or gradient system, and a variety of mobile phases have been proposed. The detection is based on optical absorption or fluorescence measurements, or makes use of mass spectrometry. Various methods of extraction of pyrimidine derivatives from biological samples are discussed, and the corresponding detection limits are presented.

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Analysis of flucytosine dosage forms by derivative UV spectroscopy and liquid chromatography

Cavrini

Bonazzi

Pietra

1991

Journal of Pharmaceutical and Biomedical Analysis

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High Performance Liquid Chromato-Graphic Assay For 5-Fluorouracil and 5-Fluorocytosine

Cited by 11 publications

References 18 publications

The Prediction of Nanoscale Drug Molecular Structure and Acid Dissociation Constants of 5-Fluorouracil in Aqueous Solution Using DFT Methods

The Prediction of Nanoscale Drug Molecular Structure and Acid Dissociation Constants of 5-Fluorouracil in Aqueous Solution Using DFT Methods

HPLC in biopharmaceutical investigations of drugs representing pyrimidine derivatives (A review)

Analysis of flucytosine dosage forms by derivative UV spectroscopy and liquid chromatography

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