High Performance Liquid Chromatographic Analysis of Soybean Phospholipids

Abstract

An HPLC method was optimized to analyze soybean phospholipids. It uses a silica column with W detection at 210 nm, and amobile phase consisting ofhexane,isopmpanol and water. Separation isachieved by keeping the hexane toisopropanolratioconstant andvaryingthewaterconcentrationina gradient elution program. Components in the HPLC chromatogram were identified by comparison with retention times of authentic compounds.The

Quantitative analysis of phospholipids by HPLC with a light scattering evaporating detector – application to raw materials for cosmetic use

“…Initially, we worked according to Sotirhos' method [9] and used a hexane/isopropanol/water mixture. The percentage of water increased during the analysis.…”

“…The percentage of water increased during the analysis. After a very good start, we encountered two problems we were unable to solve: 1) Poor resolution between PE and PA 2) Too short a lifetime of the columns, due to a rapid and In order to use less viscous and better solvent eluents, we then worked according to Erdahl and co-workers [9] with a methanolic ammonium hydroxide gradient in the chloroform. After optimization, the following binary gradient was used:…”

HPLC analysis of phospholipids by evaporative laser light‐scattering detection

“…High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds (1)(2)(3)(4)(5)(6). Several researchers have only reported on the qualitative analysis of phospholipids (1)(2)(3), while quantitative analysis requires the develop merit of calibration curves (5) or the use of phosphorimetry (4,6).…”

Lipide

“…3.11. HPLC-Analyse eines Rohlecithinsa us Soja(nach N Sotirhos et al ,1986). 1 Triacylglyceride, 2 FreieF ettsäuren, 3 Phosphatidyl-glycerin, 4 Cerebroside, 5 Phytosphingosin, 6 Diphosphatidyl-glycerin, 7 Digalactosyldiacyl-glyceride, 8 Phosphatidyl-ethanolamin, 9 Phosphatidyl-inosit, 10 Lyso-phosphatidyl-ethanolamin, 11 Phosphatidsäuren, 12 Phosphatidyl-serin, 13 Phosphatidylcholin, 14 Lyso-phosphatidylcholin…”