High-performance liquid chromatographic assay for amiodarone N-deethylation in microsomes of rat liver

Dramatic inhibition of amiodarone metabolism induced by grapefruit juice

“…detection at 242 nm, using the method described by Trivier et al. [26] with a slight modification to analyse serum amiodarone and N‐DEA. The samples were analysed after acetonitrile deproteinization on a C18 reversed‐phase 5 µm ODS Hypersil column (150 × 4.6 mm I.D.…”

Simultaneous determination of amiodarone and its metabolite desethylamiodarone by high-performance liquid chromatography with chemiluminescent detection

“…Several mobile phases have been reported for the separation of these drugs on a reverse-phase column. Most methods have used an isocratic mobile phase, typically consisting of acetonitrile or methanol combined with aqueous solutions of ammonium hydroxide [44], ammonium sulfate [45], ammonium acetate [46], sodium hydrogenphosphate [47] and acetic acid [48], although diethyl ether was used sometimes in conjunction with methanol [10,11]. The binary mixtures of methanol-aqueous ammonium sulfate adjusted to pH 6.8 were found to be suitable for the good separation of these compounds and compatible with the CL system.…”

Flow injection chemiluminescent determination of amiodarone in pharmaceutical preparations using photogenerated tris(2,2′-bipyridyl)ruthenium(III)

“…For the analysis of biological fluids, high performance liquid chromatography [5][6][7][8] and capillary electrophoresis [9,10] methods have often been proposed for the determination of amiodarone. For the determination of this drug in pharmaceutical preparations, potentiometric titration [11], spectrophotometry [12,13], infrarred spectroscopy [14], fluorimetry [15], liquid chromatography [16,17] and electrochemical methods [18,19] have been reported.…”