High performance liquid chromatographic determination of 1,5‐anhydroglucitol in human plasma for diagnosis of diabetes mellitus

Abstract

This paper describes a high performance liquid chromatographic (HPLC) method for determining 1,5-anhydroglucitol in plasma, in which anion exchange chromatography and pulsed amperometric detection are used. Plasma samples deproteinized with trichloroacetic acid are passed through a three-layer column packed with (1) strongly basic anion (BO3(3-) form, the upper layer), (2) strongly basic anion (OH- form, the middle layer) and (3) strongly acidic cation (H+ form, the lower layer) exchange resins. 1,5-Anhydroglucitol is efficiently recovered in the flow-through fraction and interfering substances are completely removed by the column treatment. The analytical response of the method is linear with concentration to 40 mg/L, and it is possible to detect as little as 0.1 mg 1,5-anhydroglucitol per litre of plasma. Analytical recovery is between 96 and 103%, and there is good agreement between the results measured by our method and by a gas/liquid chromatographic method (r = 0.998). The method has been successfully used for the determination of very low 1,5-anhydroglucitol concentrations (less than 1 mg/L) in the plasma of diabetic patients.

Reference intervals for serum 1,5‐anhydroglucitol of a population with normal glucose tolerance in Jiangsu Province

“…Glycosylated hemoglobin (HbA1c) was measured using high‐performance liquid chromatography (Bio‐RAD, Hercules, California). Levels of 1,5‐AG were measured by the GlycoMark assay (Kyowa Medex Co., Ltd., Shanghai, China) in an AU5821 automatic biochemistry analyzer (Beckman Coulter, Brea, California)， with inter‐assay and intra‐assay coefficients of variation of <2.60% and <3.10%, respectively. Estimated glomerular filtration rate (eGFR) was calculated by the Cockgroft‐Gault formula .…”

Determination of glycemic monitoring marker 1,5-anhydroglucitol in plasma by liquid chromatography-electrospray tandem mass spectrometry

“…Likewise, the results can easily be affected by endogenous interference such as glucose, conjugated bilirubin and hemoglobin [25], myo-inositol [26], maltose [27]. Therefore, other methods for plasma/serum or urine 1,5-AG measurement using chromatography techniques such as HPLC, GC/MS and LC/MS were reported [26,[28][29][30]. These methods may involve either time-consuming derivatization or laborious sample preparation procedures.…”

A novel method for the determination of 1,5-anhydroglucitol, a glycemic marker, in human urine utilizing hydrophilic interaction liquid chromatography/MS3

“…High-performance liquid chromatography (HPLC) analysis of 1,5-AG in serum and urine has also been reported. Serum levels of 1,5-AG have been determined by first passing the samples through a 2-or 3-layer ion exchange column and then analyzing by HPLC with pulsed amperometric detection or an enzyme sensor [18]. Detection of 1,5-AG indirectly through oxidation by pyranose oxidase producing hydrogen peroxide (the basis of the GlycoMark TM assay) was also applied to an HPLC platform for determination of 1,5-AG in urine [19].…”