High performance liquid chromatographic determination of methyl ethyl ketone in urine as its 3-methyl-2-benzothiazolinone hydrazone derivative

Gori, Giampaolo; Meneghetti, Paola; Sturaro, A.; Parvoli, G.; Doretti, L.; Bartolucci, Giovanni Battista

doi:10.1007/bf02290366

Cited by 6 publications

(2 citation statements)

References 9 publications

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“…Compared to methods described in the literature to assay MEK, OHMEK or 23BDs individually (Van Doorn et al, 1989;Ohmori, 1992, 1994), in the worst case the current method was 5, 12 or 13 times less sensitive, respectively. This loss in sensitivity could be related to the fact that these individual assays used larger volumes of urine and more sophisticated techniques: headspace, micro-solid phase extraction, oxidation and derivatisation steps or used a specific detector (Miyasaka et al, 1982;Gori et al, 1995;Chou et al, 1999;Antunes and Linden, 2008;Pacenti et al, 2010).…”

Section: Metabolite Assay In Urinementioning

confidence: 99%

Metabolism of inhaled methylethylketone in rats

Cosnier

Grossmann

Nunge

et al. 2017

Drug and Chemical Toxicology

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Methylethylketone (MEK) is widely used in industry, often in combination with other compounds. Although nontoxic, it can make other chemicals harmful. This study investigates the fate of MEK in rat blood, brain and urine as well as its hepatic metabolism following inhalation over 1 month (at 20, 200 or 1400 ppm). MEK did not significantly accumulate in the organism: blood concentrations were similar after six-hour or 1-month inhalation periods, and brain concentrations only increased slightly after 1 month's exposure. Urinary excretion, based on the major metabolites, 2,3-butanediols (± and meso forms), accounted for less than 2.4% of the amount inhaled. 2-Butanol, 3-hydroxy-2-butanone and MEK itself were only detectable in urine in the highest concentration conditions investigated, when metabolic saturation occurred. Although MEK exposure did not alter the total cytochrome P450 concentration, it induced activation of both CYP1A2 and CYP2E1 enzymes. In addition, the liver glutathione concentration (reduced and oxidized forms) decreased, as did glutathione S-transferase (GST) activity (at exposure levels over 200 ppm). These metabolic data could be useful for pharmacokinetic model development and/or verification and suggest the ability of MEK to influence the metabolism (and potentiate the toxicity) of other substances.

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Section: Metabolite Assay In Urinementioning

confidence: 99%

Metabolism of inhaled methylethylketone in rats

Cosnier

Grossmann

Nunge

et al. 2017

Drug and Chemical Toxicology

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“…The maximal yield of the coloured product was achieved in the pH range of 8-10 adjusted with ammonia or carbonate buffer [1]. The colour reactions with MBTH have often been applied in clinical, biochemical [40][41][42][43][44] and pharmaceutical analysis [45--481. The aim of the present paper is to elucidate the effect of structure of various substituted phenols on their reactivity with MBTH in alkaline medium and to optimize the reaction conditions for maximum coloured product formation and hence the maximum sensitivity of the spectrophotometric determination of selected phenols. N,N-dimethyl-formamide or aq.…”

mentioning

confidence: 99%

Spectrophotometric study of reactions of substituted phenols with MBTH in alkaline medium: The effect of phenol structure on the formation of analytically useful coloured products

1998

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The oxidative coupling reactions of unsubstituted phenol, 4-cresol, 2,6-xylenol and another fourteen substituted phenols with MBTH in alkaline medium containing potassium hexacyanoferrate (Ill) as the oxidant were examined by spectrophotometry. The maximum yield and stability of coloured species was attained at optimum pH 9.0-t-0.5. Stoichiometry of the reactions involving a four-electron transfer was confirmed. The coloured products were stable in an aqueous alkaline medium containing 50% (V/V) of a water-miscible organic solvent and they could be quantitatively extracted into chloroform. Absorption maxima Amax of the reaction products ranged between 495 nm and 610 nm depending on the phenol structure and their molar absorptivities fell within the range 2,500-47,000L mol-lcm -1 in aq. 50% ethanol. The calibration graphs were rectilinear for 2 to 20 gM phenol or 2,6-xylenol (r=0.9994-0.9997; n=6) and the RSD values were ~1.8% (n = 10) when determining 10 gmolL -1 of the analytes. The reactivity of the phenols with MBTH (and hence the yield of the coloured species in alkaline medium) depending on the analyte structure decreased in the order: 2,6-dialkylphenols or 4-halogenated 2,6-dialkylphenols>2-alkyl, 2-alkoxy or 2-arylphenols and 1-naphthol>unsubstituted phenol or 2-naphthol=2-halogenated phenols>4alkylphenols and 4-halogenated phenols>2-nitrophe-no1>2,4-or 3,4-dialkylphenols. For some 4-halogenated phenols the elimination of halogen upon coupling with the MBTH was observed.

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