High-performance liquid chromatographic determination of galanthamine, a long-acting anticholinesterase drug, in serum, urine and bile

Abstract

. (1983). High-performance liquid chromatographic determination of galanthamine, a long-acting anticholinesterase drug, in serum, urine, and bile. Journal of Chromatography, A, 275(2), 345-353. DOI: 10.1016/S0378-4347(00)84380-7 General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. SUMMARYThe anticholinesterase drug galanthamine is obtained from alkalinized serum by repeated liquid--liquid extraction. The resulting extract is approximately 100 times concentrated with respect to the original sample. Quantitative determination of galanthamine is performed with normal-phase liquid chromatography using a mixture of dichloromethane-n-hexane and ethanolamine as an eluent. Phenacetin is used as internal standard. The absorption of the column effluent is monitored at 235 nm. No endogenous sources of interference have been observed. A galanthamine serum level of 5 ng/ml is found as the minimum detectable concentration; the coefficient of variation at this level is 37.8% (n = 4). For the assay of galanthamine in the concentration range 10-100 ng/ml, standard deviations vary between 18.9 and 2.5% (n = 32).

Effective countermeasure against poisoning by organophosphorus insecticides and nerve agents

“…Frozen blood samples and brains were kept at Ϫ80°C until further processing. Brain and plasma levels of galantamine were measured by using a modified HPLC method (39).…”

A simple and direct HPLC-DAD method for the simultaneous determination of galantamine, donepezil and rivastigmine in cerebrospinal fluid, blood serum and urine

“…A number of methods have been developed until today for the separate determination of galantamine, donepezil and rivastigmine in plasma . Additionally, a few methods have been reported for the separate determination of these drugs in serum and urine [35][36][37][38][39]. In most of the aforementioned cases, High Pressure Liquid Chromatography, coupled with mass spectrometry or tandem mass spectrometry, was used.…”

Semi-preparative isolation and characterization of a principal oxidative degradation product in galantamine hydrobromide by LC-ESI-MSnand 2D-NMR

“…Various analytical methods were reported for the determination of galantamine, using techniques, such as micellar electrokinetic chromatography [3,4], capillary zone electrophoresis [5], HPLC-UV [6,7], HPLC-fluorescence detection or photodiode-array ra-diometric detection [8,9], high-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) [10,11,12], and validated chiral LC method, for the enantiomeric separation of galantamine [13]. Galantamine hydrobromide is also listed in both United States Pharmacopoeia (USP) [14] and European Pharmacopoeia [15].…”