High performance liquid chromatographic determination of marmelosin and psoralen in bael (Aegle marmelos (L.) Correa) fruit

Bhattacherjee, A. K.; Dikshit, Abhay; Pandey, D.; Tandon, D. K.

doi:10.1007/s13197-013-1015-x

Cited by 25 publications

(16 citation statements)

References 6 publications

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“…Each accurately weighed 5.0 g sample of homogenised bael mesocarp tissue was added to a 250 ml conical flask, then 50 ml of AR-grade benzene (SD Fine Chemicals Ltd) was added, and the flask was left overnight at room temperature (Bhattacherjee et al, 2015). Both marmelosin and psoralen were extracted from the mesocarp by shaking the mixture in an incubator shaker (E24R; Eppendorf India Ltd, Chennai, India) for 6 h at 25°C.…”

Section: Extraction Of Nutraceuticalsmentioning

confidence: 99%

“…The mobile phase consisted of a 66:34 (v/ v) mix of 100% (v/v) methanol:water at a flow rate of 1.0 ml min −1 . The detector wavelength was set at 254 nm (Bhattacherjee et al, 2015).…”

Section: Hplc Parametersmentioning

confidence: 99%

“…High performance liquid chromatography (HPLC) has been used as an accurate and sensitive method to measure marmelosin and psoralen concentrations (Bhattacherjee et al, 2015) and has been used to quantify these compounds in different cultivars of bael. Therefore, HPLC coupled with a photodiode array detector was used to measure the concentrations of marmelosin, psoralen, tannic acid, and total polyphenols in the fruit of two bael selections, CISH B-1 and CISH B-2, at various stages of fruit development.…”

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confidence: 99%

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Profiling nutraceuticals in bael [Aegle marmelos(L.) Correa] at various stages of fruit development

Bhattacherjee

Dikshit

Kumar

et al. 2016

The Journal of Horticultural Science and Biotechnology

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Section: Extraction Of Nutraceuticalsmentioning

confidence: 99%

“…The mobile phase consisted of a 66:34 (v/ v) mix of 100% (v/v) methanol:water at a flow rate of 1.0 ml min −1 . The detector wavelength was set at 254 nm (Bhattacherjee et al, 2015).…”

Section: Hplc Parametersmentioning

confidence: 99%

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confidence: 99%

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Profiling nutraceuticals in bael [Aegle marmelos(L.) Correa] at various stages of fruit development

Bhattacherjee

Dikshit

Kumar

et al. 2016

The Journal of Horticultural Science and Biotechnology

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“…We had reported a simple and reliable HPTLC method for the simultaneous determination of four coumarin derivatives, namely, umbelliferone, psoralene, marmin, and imperatorin, and an alkaloid, skimmianine, in root bark of A. marmelos [18]. Determination of coumarin derivatives like marmelosin, umbelliferone, scopoletin, and psoralene from bael fruit using HPLC has been reported [19,20]. Mass spectrometry provides rapid and sensitive quantification of biomolecules, and LC-MS has become ubiquitous as the technique of choice for many quantitative analysis applications involving pharmaceuticals and phytochemicals.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of LC—ESI—MS/MS method for simultaneous determination of four coumarin derivatives and an alkaloid from root and stem bark of Aegle marmelos Correa

Gajbhiye

Makasana

Dhanani

et al. 2016

Acta Chromatographica

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Summary.Aegle marmelos Correa (Bael tree) is a medicinal fruit tree, widely used for healing purposes in various systems of medicines. Coumarins and alkaloids present in various parts of bael tree including roots and fruit pulp are the primary active constituents implicated for its biological activities. An efficient liquid chromatographyelectrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed for identification and simultaneous determination of four coumarin derivatives, namely, umbelliferone, psoralene, marmin, and imperatorin, and an alkaloid, skimmianine, in root and stem bark of A. marmelos. The chromatographic separation of analytes was performed on Altima C18 (50 × 4.6 mm, 3 μm) column using methanol and 0.1% acetic acid in water (54:46, v/v) as the mobile phase under isocratic conditions. The LC-MS/MS parameters were optimized in the positive ionization mode using electrospray ionization source. The quantification of the analytes was performed using multiple reaction monitoring (MRM) transitions, umbelliferone (m/z 163.1 → 107.1), psoralene (m/z 187.2 → 131.1), marmin (m/z 333.5 → 163.2), imperatorin (m/z 271.1 → 203.1), and skimmianine (m/z 260.1 → 227.0). The extraction method was standardized for optimum yield of coumarin derivatives and the alkaloid in different extraction solvents. Higher yield of the analytes was found in methanolic extracts in comparisons to petroleum ether, chloroform, ethyl acetate, ethanol, and water. The method was validated for linear range, intra-and inter-batch precision and accuracy. The distribution of coumarin derivatives and an alkaloid was found to vary significantly in different plant samples, and their concentration was much higher in roots as compared to stem bark.

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“…Previously attempt has been made to develop HPLC method for individual phytochemicals and also simultaneous estimation of marmelosin and Psoralen has also been developed (Bhattacherjee et al 2013) In the present work attempt has been made to develop a sensitive, simple, and reproducible reverse phase-high performance liquid chromatography (RP-HPLC) method with good recovery for determination of three coumarins isolated from methanolic extract of A. marmelos fruit. RP-HPLC method offers a number of advantages for the analysis of herbals extract and is suitable for comparison of samples based on finger prints.…”

Section: Introductionmentioning

confidence: 99%

Rapid simultaneous determination of marmelosin, umbelliferone and scopoletin from Aegle marmelos fruit by RP-HPLC

et al. 2014

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The surge of interest in naturally occurring phytochemicals with high therapeutic potential has led to the discovery of many molecules, out of which naturally occuring coumarins such as marmelosin, umbelliferone and scopoletin present in Aegle marmelos (Bael) fruit shows good therapeutic potential. The aim of the present work is to develop and validate Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for simultaneous determination of marmelosin, umbelliferone and scopoletin in A. marmelos fruit extracts. The chromatographic separation was performed with isocratic elution of 55:45 (%, v/v) methanol-water containing 0.1 % acetic acid as mobile phase. The method used to analyse the extract of A. marmelos showed good resolution with retention time within 12 min. The relative concentrations of above phytoconstituent were determined in A. marmelos fruits. The method was found to give compact peaks for scopoletin, umbelliferone and marmelosin (R t of 4.6, 6.5 and 11.3 min respectively) and were linear over the range 5-30 μg ml −1 (R 2 =0.9655), 2-10 μg ml −1 (R 2 = 0.9964) and 2-10 μg ml −1 (R 2 =0.9862) respectively. The mean recoveries for marmelosin, umbelliferone and scopoletin at three concentrations were in the range of 98.8-102.9, 98.8-101.1 and 94.2-98.3 % respectively. The relative standard deviation of accuracy, precision and repeatability were within 2 %, indicating the method produced highly reproducible results. Therefore this simple, precise and accurate method enables simultaneous separation of this phytoconstituent and hence can be successfully applied in analysis and routine quality control of herbal material and formulation containing A. marmelos.

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High performance liquid chromatographic determination of marmelosin and psoralen in bael (Aegle marmelos (L.) Correa) fruit

Cited by 25 publications

References 6 publications

Profiling nutraceuticals in bael [Aegle marmelos(L.) Correa] at various stages of fruit development

Profiling nutraceuticals in bael [Aegle marmelos(L.) Correa] at various stages of fruit development

Development and validation of LC—ESI—MS/MS method for simultaneous determination of four coumarin derivatives and an alkaloid from root and stem bark of Aegle marmelos Correa

Rapid simultaneous determination of marmelosin, umbelliferone and scopoletin from Aegle marmelos fruit by RP-HPLC

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