High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits

Amakura, Yoshiaki; Okada, Mai; Tsuji, Sumiko; Tonogai, Yasuhide

doi:10.1016/s0021-9673(00)00414-3

Cited by 179 publications

(112 citation statements)

References 20 publications

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“…Plants products or ingredients of seeds, acids such as gallagic acid, ellagic acid and EA glycosides. 19 Additionally, seeds hold constituents such as protein, crude fibers, vitamins, minerals, sugars, polyphenols, the phytoestrogen coumestrol estrone. [20][21][22][23] Pharmalogical Activities of Pomegranate Antioxidant Activity…”

Section: Anti-microbial Activitymentioning

confidence: 99%

Active Constituents of Pomegranates (Punica granatum) as Potential Candidates in the Management of Health through Modulation of Biological Activities

Rahmani¹,

Alsahli²,

Almatroodi³

2017

190

183

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Pomegranates fruits have innumerable health benefits and its implication in diseases cure have been widely recognized since ancient time. Moreover, pomegranate fruits, seeds and peels are intensively used in traditional medicine as a natural therapy. It contains numerous valuable ingredients such as flavonoids, ellagitannin, punicalagin, ellagic acid, vitamins and minerals. The principal constituents including punicalagins and ellagitannin are responsible for immeasurable health benefits due to its strong antioxidant activity. Additionally, constituents of pomegranate show health promoting effect through the modulation of physiological and biochemical pathways. Recent evidences suggested that pomegranates fruits, peels and seeds illustrate therapeutics implications in health management via inhibition of free radical effect and modulation of enzymes activity linked with diseases development and progression. In this review, we summarize the therapeutic role of pomegranate fruits, seeds and peels in the health managements based on in vitro and in vivo studies.

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Section: Anti-microbial Activitymentioning

confidence: 99%

Active Constituents of Pomegranates (Punica granatum) as Potential Candidates in the Management of Health through Modulation of Biological Activities

Rahmani¹,

Alsahli²,

Almatroodi³

2017

190

183

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“…GTscan also be transformed to ETs by oxidative C-C coupling between spatially adjacent galloyl groups to form HHDP groups. ETs are known to be abundant in berry fruits and after hydrolytic conversion, are commonly detected and quantified in the form of EA (Amakura, Okada, Tsuji, & Tonogai, 2000). Hence EA may be considered as a chemical marker compound for hydrolyzable tannins.…”

Section: Hydrolyzable Tannins (Ets Gts and Ea)mentioning

confidence: 99%

Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy

et al. 2006

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Strawberry (Fragaria x ananassa Duch.) fruits contain phenolic compounds that have antioxidant, anticancer, antiatherosclerotic and anti-neurodegenerative properties. Identification of food phenolics is necessary since their nature, size, solubility, degree and position of glycosylation and conjugation influence their absorption, distribution, metabolism and excretion in humans. Freezedried whole strawberry fruit powder and strawberry fruit extracts were analyzed by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) methods. Phenolics were identified as ellagic acid (EA), EA-glycosides, ellagitannins, gallotannins, anthocyanins, flavonols, flavanols and coumaroyl glycosides. The anthocyanidins were pelargonidin and cyanidin, found predominantly as their glucosides and rutinosides. The major flavonol aglycons were quercetin and kaempferol found as their glucuronides and glucosides. LC-ESI-MS/MS methods differentiated EA from quercetin conjugates since both aglycons have identical molecular weights (302 g/mol). The identification of strawberry phenolics is necessary to generate standardized materials for in vitro and in vivo studies and for the authentication of strawberry-based food products.

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“…Among these phytochemicals, ellagic acid (EA), a dimeric derivative of gallic acid, occurs in fruits and nuts in either its free form, as EA-glycosides, or bound as ellagitannins (ETs) [1,2]. The absorption, bioavailability and pharmacokinetics of EA administered orally have not been adequately investigated [3] and to the best of our knowledge have never been studied in the human body.…”

Section: Introductionmentioning

confidence: 99%

Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice

Seeram

Lee

Heber

2004

Clinica Chimica Acta

392

273

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Background: Ellagic acid (EA) and hydrolyzable ellagitannins (ETs) are dietary polyphenols found in fruits and nuts and implicated with potent antioxidant, anticancer and antiatherosclerotic biological properties. Unfortunately, there are no reports on the bioavailability studies of EA or ETs in the human body. We conducted in vivo studies whereby a human subject consumed pomegranate juice (PJ) (180 ml) containing EA (25 mg) and ETs (318 mg, as punicalagins, the major fruit ellagitannin). Methods: A rapid plasma extraction procedure utilizing acidic precipitation of proteins, followed by HPLC-UV analyses, was employed. Results: EA was detected in human plasma at a maximum concentration (31.9 ng/ml) after 1 h postingestion but was rapidly eliminated by 4 h. The calibration curve for quantification of EA was linear (r 2 = 0.9975) over the concentration range from 1000 to 15.6 ng/ml. Conclusions: Since EA has reportedly strong affinity for proteins and poor absorption in small animals, further studies to investigate whether the presence of free EA in human plasma may be due to its release from the hydrolysis of ETs, facilitated by physiological pH and/or gut microflora action, is warranted. EA can be considered as a biomarker for future human bioavailability studies involving consumption of ETs from food sources. D

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High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits

Cited by 179 publications

References 20 publications

Active Constituents of Pomegranates (Punica granatum) as Potential Candidates in the Management of Health through Modulation of Biological Activities

Active Constituents of Pomegranates (Punica granatum) as Potential Candidates in the Management of Health through Modulation of Biological Activities

Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy

Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice

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