High Performance Liquid Chromatographic Method for Determination of Sildenafil Citrate (Viagra) in Pharmaceutical Formulation and Industrial Effluent Sample

Abstract

A simple, precise, rapid and accurate reversed-phase high performance liquid chromatographic method has been developed for the determination of sildenafil citrate in a pure form, pharmaceutical formulations and industrial effluent samples. Chromatography was carried out on supelco L 7 reversed-phase column (25cm × 4.6mm), 5 microns, using a mixture of methanol: water: triethylamine (630: 370 :2 v/v/v) and adjusted to pH 4.0 with dilute phosphoric acid as a mobile phase at a flow rate of 1.0 ml.min-1 .Uv detection was performed at 220 nm at ambient temperature (25 °C). The retention time for sildenafil citrate found to be 4.6 minutes. The calibration curve was linear (r= 0.9998) over a concentration range from 10 to 70 µg/ml. Limit of detection (LOD) and limit of quantification (LOQ) were 60 ng/ml and 180 ng/ml respectively. The method was validated for its linearity, precision and accuracy. The proposed method was successfully applied to the determination of sildenafil citrate in tablets and industrial effluent samples.

HPLC Determination of Sildenafil in Tablets

“…Various analytical techniques like spectrophotometry [9,11], Raman spectroscopy [11,12], thin layer chromatography [13], high performance liquid chromatography [14][15][16][17][18][19][20][21][22][23], high performance liquid chromatography-mass spectrometry [24][25][26][27] are reported for Sildenafil determination in pharmaceutical preparations [10, 12, 18-20, 22, 23, 25], biological samples [16,20,21,[24][25][26][27], dietary supplements [3,5,13], herbal products [15] and drinks [11].…”