High-performance liquid chromatographic method for determination of gentamicin in biological fluids

Walker, S.; Coates, P. E.

doi:10.1016/s0378-4347(00)80075-4

Cited by 33 publications

(11 citation statements)

References 8 publications

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“…Tobramycin in the samples of aqueous humor, vitreous humor and retina was quantified by a reverse-phase HPLC method [25][26][27]42] with a UV detector (λ = 350 nm) using 1-fluoro 2,4-dinitrobenzene to derivatize tobramycin. The mobile phase based on acetonitrile:phosphoric acid (5.0 g/L) 70:30 v/v was pumped in HPLC systems (Shimadzu, Italy) consisted of a LC-10 AC pump, 20 μL loop injector (Reodyne, Sigma-Aldrich, Italy) and a C-R6A recorder.…”

Section: Quantitative Determination Of Tobramycinmentioning

confidence: 99%

Solid lipid nanoparticles as promising tool for intraocular tobramycin delivery: Pharmacokinetic studies on rabbits

Chetoni

Burgalassi

Monti

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

136

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Eye drops are widely accepted as formulations for targeting the anterior segment notwithstanding their limitations in terms of bioavailability. The unique structure of the eye requires specially-designed formulations able to favor the pharmacokinetic profile of administered drugs, mainly minimizing the influence of ocular barriers. Nanotechnology-based delivery systems lead to significant technological and therapeutical advantages in ophthalmic therapy. The aim of the present study was to determine whether tobramycin as ion-pair incorporated in mucoadhesive Solid Lipid Nanoparticles (SLN) reaches the inner parts of the eye favoring drug activity. After technological characterization of the tobramycin entrapped SLN formulation (Tobra-SLN), a pharmacokinetic study in rabbits after topical instillation and intravenous administration of the formulation has been carried out. In addition, the intracellular activity of Tobra-SLN formulation against phagocytosed Pseudomonas aeruginosa was investigated. The SLN were spherical in shape, and showed a hydrodynamic diameter of about 80nm, a negative zeta potential (-25.7mV) with a polydispersity index of 0.15, representative of a colloidal dispersion with high quality, characterized by an unimodal relatively narrow size distribution. As demonstrated by FTIR and DSC, tobramycin ion-pair could be concentrated into lipid inner core of SLN, without interaction with the stearic acid, thus promoting a slow and constant drug release profile in the dissolution medium. Surprisingly, the drug concentration was significantly higher in all ocular tissues after ocular and intravenous administration of Tobra-SLN formulation with respect to reference formulations and only Tobra-SLN allowed the penetration of drug into retina. Furthermore, the use of Tobra-SLN resulted in both higher intraphagocytic antibiotic concentrations in polymorphonuclear granulocytes and greater bactericidal activity against intracellular Pseudomonas aeruginosa, probably due to the ability of Tobra-SLN to penetrate either into phagocytic cells, or alternatively to cross bacterial barrier. The present study broadens the knowledge on the use of SLN as carriers for ocular drug delivery to the posterior chamber and might open new avenues for treatment of ocular infections, representing a strategy to overcome the microbial resistance.

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Section: Quantitative Determination Of Tobramycinmentioning

confidence: 99%

Solid lipid nanoparticles as promising tool for intraocular tobramycin delivery: Pharmacokinetic studies on rabbits

Chetoni

Burgalassi

Monti

et al. 2016

European Journal of Pharmaceutics and Biopharmaceutics

136

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“…25 First, 100 mL of plasma and 800 mL of 0.05 mol L À1 potassium phosphate (pH 4.5) were mixed with 50 mL of 1.0 mol L À1 sodium hydroxide in a test tube. After briefly vortexing, 2.5 mL of acetonitrile were added.…”

Section: Plasma Sample Preparationmentioning

confidence: 99%

Duodenal administration of solid lipid nanoparticles loaded with different percentages of tobramycin

Cavalli

Bargoni

Podio

et al. 2003

Journal of Pharmaceutical Sciences

117

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“…Solvents that were used for PPT included acetonitrile for the extraction of netilmicin, amikacin, tobramycin, gentamicin, etimicin, neomycin, and isepamicin [6,15,21,23,32,[37][38][39][40]42,68,[73][74][75][76]78,79,82,86]; trichloroacetic acid for the extraction of geneticin, spectinomycin, tobramycin, gentamicin, kanamycin, hygromycin, apramycin, streptomycin, dihydrostreptomycin, amikacin, neomycin, and paromomycin [29,34,35,57,61,72,81]; methanol for the extraction of geneticin, amikacin, tobramycin, sisomicin, and netilmicin [29,41,48]; perchloric acid for the extraction of kanamycin, dibekacin, arbekacin, and streptomycin [26,33,49,51]; formic acid for the extraction of amikacin [84,85]; and methylene chloride for the extraction of isepamicin [62]. PPT aids in reducing interference during derivatization [7], and this may have accounted for its use by 41 articles.…”

Section: Sample Cleanupmentioning

confidence: 99%

Bioanalysis of aminoglycosides using high-performance liquid chromatography

et al. 2022

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Aminoglycosides are broad-spectrum antibiotics used in the treatment of gram-negative bacterial infections. Due to their nephrotoxic and ototoxic potential (narrow therapeutic index), the use of aminoglycoside for clinical indications requires monitoring. The objective of this review was to identify relevant literature reporting liquid chromatographic methods for the bioanalysis of aminoglycosides in both preclinical and clinical settings/experiments. Data on liquid chromatographic methods were collected from articles in an online academic database (PubMed, Science Direct, Scopus, and Google Scholar). All 71 articles published from 1977 to 2020 were included in the review. Reversed-phase liquid chromatography was the most used method for the bioanalysis of aminoglycosides. Fluorescence or ultraviolet detection methods were mostly used from 1977 to 2002 (51 articles), while mass spectrometry was predominantly used as a detector from 2003 to 2020 (15 articles). Sixty-seven articles reported calibration ranges, which varied significantly for the various drugs assayed: some in the range of 0.1-0.5 ng/mL and others 1250-200000 ng/mL. Also, 61 articles reported R2 values (0.964-1.0) for almost all analytes under consideration. Sixty-three articles reported percent recoveries mostly between 61.0 % to 114.0 %, with only two articles reporting recoveries of 4.9 % and 36 %. Out of the 71 reviewed articles, 56 reported intermediate precision values ranging between 0.331 % to 19.76 %, which is within the acceptable limit of 20 %. This review will serve as a guide for research and/or routine clinical monitoring of aminoglycosides in biological matrices.

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High-performance liquid chromatographic method for determination of gentamicin in biological fluids

Cited by 33 publications

References 8 publications

Solid lipid nanoparticles as promising tool for intraocular tobramycin delivery: Pharmacokinetic studies on rabbits

Solid lipid nanoparticles as promising tool for intraocular tobramycin delivery: Pharmacokinetic studies on rabbits

Duodenal administration of solid lipid nanoparticles loaded with different percentages of tobramycin

Bioanalysis of aminoglycosides using high-performance liquid chromatography

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