High-performance liquid chromatographic separation and nanogram quantitation of bupivacaine enantiomers in blood

Gu, Xiao; Fryirs, Bronwyn; Mather, Laurence E.

doi:10.1016/s0378-4347(98)00380-6

Cited by 24 publications

(11 citation statements)

References 13 publications

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“…The recovery of bupivacaine enantiomers added to plasma was greater than 70%, irrespective of the concentration. Extraction of bupivacaine in basic medium containing n-hexane, after plasma deproteinization with acetonitrile, resulted in a recovery rate similar to that reported by Gu et al, 26 who used direct extraction with n-hexane. The previous plasma deproteinization step was found to be fundamental in obtaining chromatograms free of interference from endogenous components (Fig.…”

Section: Discussionsupporting

confidence: 77%

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Stereoselectivity in the placental transfer and kinetic disposition of racemic bupivacaine administered to parturients with or without a vasoconstrictor

et al. 2003

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The aim of the present study was to investigate the stereoselectivity in the kinetic disposition and the transplacental distribution of bupivacaine in term parturients during labor. Maternal age ranged from 18-37 years and fetal gestational age from 37.6-41.5 weeks. Healthy parturients (n = 23) received epidural 0.5% racemic bupivacaine alone (group A) or combined with epinephrine (group B). Maternal venous blood was sampled at regular intervals until 8 h after drug administration and umbilical venous blood was obtained at delivery. Bupivacaine enantiomers were determined in plasma samples by HPLC using a Chiralcel(R) OD-R column and a UV detector. One- or two-compartment models were fitted to data and differences between the (+)-(R) and (-)-(S) enantiomers were compared with the paired Wilcoxon test (P< 0.05). The influence of epinephrine was evaluated using the unpaired Mann-Whitney test (P< 0.05). The disposition of bupivacaine in maternal plasma was stereoselective, with higher V(d/f) (140.60 vs. 132.81 L for group A and 197.86 vs. 169.46 L for group B) and C(l/f) (29.00 vs. 25.43 L/h for group A and 33.15 vs. 26.39 L/h for group B) and lower t(1/2)beta (3.24 vs. 3.30 h for group A and 4.36 vs. 4.45 h for group B) being observed for (+)-(R)-bupivacaine. The combined administration of epinephrine resulted in higher V(d/f) (197.86 vs. 140.60 L for (+)-(R) and 169.46 vs. 132.81 L for (-)-(S)) and t(1/2)beta values (4.36 vs. 3.24 h for (+)-(R) and 4.45 vs. 3.30 h for (-)-(S)). The transplacental distribution of bupivacaine was stereoselective only when bupivacaine was administered without epinephrine (group B), with a higher cord blood/maternal blood ratio being observed for (-)-(S)-bupivacaine (0.40 vs. 0.35). Chirality 16:65-71, 2004.

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Section: Discussionsupporting

confidence: 77%

“…The quantification limit of 5 ng obtained for each enantiomer/ml plasma (Table 1) suggests that the method is more sensitive than that reported by Gu et al, 26 who obtained a quantification limit of 15 ng/ml for each enantiomer.…”

Section: Discussionmentioning

confidence: 58%

Stereoselectivity in the placental transfer and kinetic disposition of racemic bupivacaine administered to parturients with or without a vasoconstrictor

et al. 2003

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“…Stereoselective effects for racSal and rac-Bup in 30 min administration metabolism were not observed. These results were in agreement with the literatures [8,9], which inhaled after administration of rac-Sal [8] and after a 3 min infusion rac-Bup into the left coronary artery of a sheep [9].…”

Section: Analytical Applicationsupporting

confidence: 93%

“…A number of methods have been developed for chiral separations of salbutamol or bupivacaine included high-performance liquid chromatography (HPLC) [8,9], gas chromatography (GC) [7,10] and, more recently capillary electrophoresis (CE) [11][12][13]. In CE, the most widely used chiral selectors are cyclodextrins (CD).…”

Section: Introductionmentioning

confidence: 99%

Chiral separation of salbutamol and bupivacaine by capillary electrophoresis using dual neutral cyclodextrins as selectors and its application to pharmaceutical preparations and rat blood samples assay

Wei

Guo

Lin

2006

Journal of Chromatography B

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“…Enantioselective activity and pharmacokinetics have been described for bupivacaine [13][14][15]. Yet, determination of the sum of the enantiomers concentrations after epidural administration of the racemate can still provide information of interest [4].…”

Section: Introductionmentioning

confidence: 99%

Sensitive bioassay of bupivacaine in human plasma by liquid-chromatography-ion trap mass spectrometry

Hoizey

Lamiable

Robinet

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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High-performance liquid chromatographic separation and nanogram quantitation of bupivacaine enantiomers in blood

Cited by 24 publications

References 13 publications

Stereoselectivity in the placental transfer and kinetic disposition of racemic bupivacaine administered to parturients with or without a vasoconstrictor

Stereoselectivity in the placental transfer and kinetic disposition of racemic bupivacaine administered to parturients with or without a vasoconstrictor

Chiral separation of salbutamol and bupivacaine by capillary electrophoresis using dual neutral cyclodextrins as selectors and its application to pharmaceutical preparations and rat blood samples assay

Sensitive bioassay of bupivacaine in human plasma by liquid-chromatography-ion trap mass spectrometry

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