High-performance liquid chromatography coupled with post-column dual-bioactivity assay for simultaneous screening of xanthine oxidase inhibitors and free radical scavengers from complex mixture

Li, D.Q.; Zhao, Jing; Li, S.P.

doi:10.1016/j.chroma.2014.03.065

Cited by 46 publications

(13 citation statements)

References 25 publications

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“…The ABTS radical scavenging assay was performed according to Li’s method [20] with some modification. ABTS radical cation (ABTS• + ) was produced by reacting ABTS solution (7 mM in water) with 2.5 mM potassium persulfate for 12 h with a ratio of 2:1 ( v / v ) at 4 °C in dark (stock solution).…”

Section: Methodsmentioning

confidence: 99%

“…Above all, the action and effects of natural antioxidants contained in foods have been the subjects of extensive studies [16,17,18]. The activity of antioxidants is usually measured by DPPH-RSA, ABTS-RSA, FRAP assay and CUPRAC (cupric reducing antioxidant capacity) assay [19,20,21]. Since the antioxidant activity of F. thunbergii bulbs has not been critically evaluated, its antioxidant activity was measured using three different methods (DPPH-RSA, ABTS-RSA and FRAP assays) to ascertain their potential as functional foods and functional ingredients in this paper.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

et al. 2016

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Supercritical fluid extraction (SFE) was used to extract total alkaloids, peimisine, peimine and peiminine from the bulb of Fritillaria thunbergii Miq. The antioxidant capacity of the extracts was evaluated by DPPH radical scavenging activity (DPPH-RSA), ABTS radical scavenging activity (ABTS-RSA) and ferric reducing capacity (FRAP) assay. A central composite design (CCD) with four variables and five levels was employed for optimization of process parameters, and response surface plots were constructed in accordance with a second order polynomial model. Under optimal conditions of 3.0 h, 60.4 °C, 26.5 MPa and 89.3% ethanol, the highest yields were predicted to be 3.8 mg/g for total alkaloids, 0.5 mg/g for peimisine, 1.3 mg/g for peimine and 1.3 mg/g for peiminine, and the antioxidant capacity of extracts displayed EC50, DPPH value of 5.5 mg/mL, EC50, ABTS value of 0.3 mg/mL and FRAP value of 118.2 mg ascorbic acid equivalent (AAE)/100 g.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

et al. 2016

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“…Oroxin B is one of the major bioactive components in Semen Oroxyli. Some assays with high‐performance liquid chromatography (HPLC) methods have been reported for the quantitative determination of oroxin B in Semen Oroxyli extracts or related pharmaceutical formulations (; ; Cao, Yan, Yang, & Huang, ; Cheng & Liu, ; Li, Wei, & Chen, ; Zhang et al, ). However, these methods have their own disadvantages, such as low specificity, poor sensitivity and long chromatographic run times, and so are not suitable for the analysis of oroxin B in biological samples.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay

Niu

Yan

Guo

et al. 2018

Biomedical Chromatography

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A highly sensitive LC-MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative-ionization mass spectrometry in selectedreaction monitoring mode. The assay was linear in the concentration range 4.52-904 ng/mL with intra-and inter-day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.

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“…In the past decade, several methods have been developed for discovery of active compounds from natural products such as ultrafiltration , TLC bioautography , HPLC combined with biochemical detection , etc. However, they cannot be used for preliminary screening that is the important step for supplying high quality and potential active natural extracts for future drug development.…”

Section: Introductionmentioning

confidence: 99%

Practical method for the rapid screening of xanthine oxidase inhibitors in herbal extracts by high‐performance liquid chromatography based on on‐line precolumn enzymatic reaction

Zhou

Xiao

et al. 2015

J of Separation Science

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A high-performance liquid chromatography method with on-line precolumn enzymatic reaction for the screening of xanthine oxidase inhibitors in natural extracts was developed. In this method, the enzymatic reaction occurred at the capillary inlet during a predetermined waiting period, after which the reaction product, uric acid, was separated and detected by liquid chromatography using ultraviolet absorption at 295 nm. Enzyme inhibition can be read out directly from the reduced peak area of uric acid in comparison to a reference chromatogram obtained in the absence of any inhibitor. In the present study, the availability of on-line precolumn enzymatic reaction with ultraviolet detection was firstly evaluated by determining the inhibitory mechanism and IC50 values of allopurinol, a commercially available positive drug. Then, the newly developed method was applied to screening of ten natural extracts from traditional Chinese medicine and as a result, the extract of Epimedium sagittatum (Sieb. et Zucc.) Maxim was found to be most positive for xanthine oxidase inhibition. The results obtained were compared with those obtained by offline enzyme assay and the effectiveness of the present method was confirmed. A rapid, low-cost, and fully automated method for xanthine oxidase inhibitor screening was proposed.

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High-performance liquid chromatography coupled with post-column dual-bioactivity assay for simultaneous screening of xanthine oxidase inhibitors and free radical scavengers from complex mixture

Cited by 46 publications

References 25 publications

Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

Optimization of Supercritical Fluid Extraction of Total Alkaloids, Peimisine, Peimine and Peiminine from the Bulb of Fritillaria thunbergii Miq, and Evaluation of Antioxidant Activities of the Extracts

Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay

Practical method for the rapid screening of xanthine oxidase inhibitors in herbal extracts by high‐performance liquid chromatography based on on‐line precolumn enzymatic reaction

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