High‐performance liquid chromatography/electrospray ionisation mass spectrometric characterisation of metabolites produced by <i>Pseudovibrio</i> sp. W64, a marine sponge derived bacterium isolated from Irish waters

Choudhary, Alka; Naughton, Lynn M.; Dobson, Alan D. W.; Dilip, K.

doi:10.1002/rcm.8226

Cited by 8 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was previously found as the product of Pseudovibrio sp. W64 marine sponge-derived bacterium [32], and it showed biological activities including antiseptic, anti-inflammatory [33] and antimicrobial activity [34]. Oleamide (amide of oleic acid, entry 5, Tables 1 and 2) was the most abundant compound in F3 of FdEc and F4 of FdEa.…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Mature zebrafish Danio rerio (a wild-type strain obtained from European Zebrafish Resource Center, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany) were utilized for egg production, following the procedure [32].…”

Section: Zebrafish Embryotoxicity Testmentioning

confidence: 99%

See 2 more Smart Citations

Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity

et al. 2022

View full text Add to dashboard Cite

Ericaria crinita and Ericaria amentacea from the Adriatic Sea (Croatia) were investigated with respect to the presence of less-polar compounds for the first time after fractionation by solid-phase extraction (SPE). The composition of less-polar fractions of freeze-dried E. crinita (FdEc) and E. amentacea (FdEa) were analyzed by high-performance liquid chromatography–high-resolution mass spectrometry with electrospray ionization (UHPLC-ESI-HRMS). The major identified compounds were: amides of higher aliphatic acids (palmitoleamide, linoleamide, palmitamide, oleamide and erucamide) and related compounds, carotenoid (fucoxanthin), chlorophyll derivatives (pheophytin a and b and their derivatives) and higher terpenes (loliolide, isoamijiol with its oxidation product), β-stigmasterol and (3β,6α)-14-methylergosta-8,24(28)-diene-3,6-diol). The toxic effects observed on the less-polar fractions obtained from Ericaria species on zebrafish Danio rerio embryos could be associated with the high abundance of all five detected amides. The antioxidant activity of the fractions was evaluated by means of five independent assays, including the reduction of the radical cation (ABTS), the oxygen radical absorbance capacity (ORAC), ferric-reducing antioxidant power (FRAP), the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay and the Folin–Ciocalteu method. A higher antioxidant activity of E. amentacea in comparison to that of the E. crinita fractions was found with IC50 concentrations of 0.072 and 1.177 mg/mL, respectively. The correlation between the activity and the chemical composition revealed that the synergistic effect of different compounds impacted their antioxidant response.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…LC-MS characterization was carried out on a Q-Tof Premier mass spectrometer (Waters Corporation, Milford, MA, USA) coupled to an Alliance 2695 HPLC system (Waters Corporation, Milford, MA, USA) as described previously [ 59 , 60 ]. Accurate mass measurements of the analytes were achieved through the use of an internal reference compound (leucine-enkephalin).…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

et al. 2018

Self Cite

View full text Add to dashboard Cite

Antioxidant phytochemicals play a key role in oxidative stress control and in the prevention of related disorders, such as premature aging, degenerative diseases, diabetes, and cancer. The aim of this study was to investigate the potential antioxidant activity and the phytochemical profile of Senecio clivicolus Wedd., a perennial shrub, belonging to the Asteraceae family. Despite the wide interest of this family, this specie has not been investigated yet. S. clivicolus aerial parts were extracted with 96% ethanol. Then, the ethanol extract was fractionated by liquid/liquid extraction using an increasing solvents polarity. Total polyphenol and terpenoid contents were measured. Moreover, the antioxidant activity was evaluated by six different complementary in vitro assays. The Relative Antioxidant Capacity Index (RACI) was used to compare data obtained by different tests. The sample showing the highest RACI was subjected to characterization and quantitation of its phenolic composition using LC-MS/MS analysis. The ethyl acetate fraction, investigated by LC-MS/MS analysis, showed 30 compounds, most of them are chlorogenic acid and flavonoid derivatives. To the best of our knowledge, this is the first report about the evaluation of antioxidant activity and phytochemical profile of S. clivicolus, underlying the importance of this species as a source of health-promoting phytochemicals.

show abstract

“…No caso de PNs obtidos a partir de esponjas marinhas, alguns já foram atribuídos como produtos de biossíntese de microrganismos (Figuras 1.4-1.6) (HENTSCHEL et al, 2012;MCCAULEY et al, 2020;STILL et al, 2014 BONDAREV et al, 2013;NAUGHTON et al, 2017;ROMANO, 2018;ROMANO et al, 2014ROMANO et al, , 2016SCHWEDT et al, 2015;VERSLUIS et al, 2018 ilustram a versatilidade metabólica de Pseudovibrio spp. (CHOUDHARY et al, 2018;HARRINGTON et al, 2014;NICACIO et al, 2017;PENESYAN et al, 2011;RAINA et al, 2016;RODRIGUES et al, 2017;SERTAN-DE GUZMAN et al, 2007;VIZCAINO, 2011;WANG et al, 2015) .…”

Section: Biossíntese De Produtos Naturaisunclassified

Descoberta e estudos de biossíntese de peptídeos não-ribossomais envolvidos na motilidade de >i<Pseudovibrio brasiliensis>/i< Ab134

Ióca¹

View full text Add to dashboard Cite

pelas oportunidades, orientação e apoio, ao longo dos últimos 10 anos, responsável pela minha formação acadêmico-científica. À Profa. Dra. Alessandra S. Eustáquio (The University of Illinois at Chicago, UIC), por me receber em seu grupo de pesquisa, sempre disponível para discussões e guiando cada tomada de decisão. Obrigada pela orientação, paciência e conselhos. A todos os colegas do Grupo de Química Orgânica de Sistemas Biológicos pela amizade, sugestões e companhia nas longas semanas de HPLC e desenvolvimento de experimentos de cultivos intermináveis. Em especial, à Dra. Fabiana T. R. Martinelli e Dra. Karin F. B. Camargo pelo apoio técnico. Aos Prof. Dr. Alekej Krunic (UIC), Profa. Dra. Laura M. Sanchez (UIC) e Prof. Dr. Antônio G. Ferreira (Universidade Federal de São Carlos, UFSCar) pelo apoio técnico na aquisição de dados de ressonância magnética nuclear e espectrometria de massas. Aos amigos, antigos e novos, que fizeram essa caminhada mais acolhedora, principalmente a Carol

show abstract

High‐performance liquid chromatography/electrospray ionisation mass spectrometric characterisation of metabolites produced by Pseudovibrio sp. W64, a marine sponge derived bacterium isolated from Irish waters

Cited by 8 publications

References 47 publications

Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity

Bioprospecting of Less-Polar Fractions of Ericaria crinita and Ericaria amentacea: Developmental Toxicity and Antioxidant Activity

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

Descoberta e estudos de biossíntese de peptídeos não-ribossomais envolvidos na motilidade de >i<Pseudovibrio brasiliensis>/i< Ab134

Contact Info

Product

Resources

About