High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Ceiba speciosa Growing in Egypt

Abdel-Aziz, Abdel-Wanes Anter; Elwan, Nehal M.; Abdallah, Magda A.; Shaaban, Rasha Shaaban; Osman, Nadia S.; Mohamed, Mona AbelMotegally

doi:10.21608/ejchem.2021.58716.3267

Cited by 12 publications

(9 citation statements)

References 14 publications

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“…Each sample’s phenolic and flavonoid composition was determined by comparing their retention times and spectral reference data with the external standard controls. All standards, namely gallic acid, chlorogenic acid, catechin, methyl gallate, caffeic acid, syringic acid, pyrocatechol, rutin, ellagic acid, coumaric acid, vanillin, ferulic acid, naringenin, querectin, cinnamic acid, kaempferol, and hesperetin were purchased from Sigma-Aldrich ( Khalil et al, 2020 ; Abdel-Aziz et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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Oxidative stress is involved in the pathophysiology of multiple health complications, and it has become a major focus in targeted research fields. As known, black seeds are rich sources of bio-active compounds and widely used to promote human health due to their excellent medicinal and pharmaceutical properties. The present study investigated the antioxidant potency of various black seeds from plants and their derived mycoendophytes, and determined the total phenolic and flavonoid contents in different extracts, followed by characterization of major constituents by HPLC analysis. Finally, in silico docking determined their binding affinities to target myeloperoxidase enzymes. Ten dominant mycoendophytes were isolated from different black seed plants. Three isolates were then selected based on high antiradical potency and further identified by ITS ribosomal gene sequencing. Those isolated were Aspergillus niger TU 62, Chaetomium madrasense AUMC14830, and Rhizopus oryzae AUMC14823. Nigella sativa seeds and their corresponding endophyte A. niger had the highest content of phenolics in their n-butanol extracts (28.50 and 24.43 mg/g), flavonoids (15.02 and 11.45 mg/g), and antioxidant activities (90.48 and 81.48%), respectively, followed by Dodonaea viscosa and Portulaca oleracea along with their mycoendophytic R. oryzae and C. madrasense. Significant positive correlations were found between total phenolics, flavonoids, and the antioxidant activities of different tested extracts. The n-butanol extracts of both black seeds and their derived mycoendophytes showed reasonable IC50 values (0.81–1.44 mg/ml) compared to the control with significant correlations among their phytochemical contents. Overall, seventeen standard phenolics and flavonoids were used, and the compounds were detected in different degrees of existence and concentration in the examined extracts through HPLC analysis. Moreover, the investigation of the molecular simulation results of detected compounds against the myeloperoxidase enzyme revealed that, as a targeted antioxidant, rutin possessed a high affinity (−15.3184 kcal/mol) as an inhibitor. Taken together, the black seeds and their derived mycoendophytes are promising bio-prospects for the broad industrial sector of antioxidants with several valuable potential pharmaceutical and nutritional applications.

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Section: Methodsmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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“…The antioxidant potential of C. speciosa leaves ethanolic was 78.7 % for DPPH radical scavenging (at 1 mg/mL), with this activity attributed to the presence of phenolic compounds, especially flavonoids (Nasr et al, 2018). Methanolic extract of leaves and its dichloromethane fraction presented antioxidant potential similar to the standard, with IC 50 of 15.48 and 12.37 µg/mL, compared to ascorbic acid (7.60 µg/mL) (Abdel-Aziz et al, 2021). Seed fixed oil showed strong IC 50 values, with 10.21 µg/mL and 77.44 µg/mL for ABTS and DPPH radical scavenging activity assays, respectively (Rosselli et al, 2020).…”

Section: Ceiba Speciosamentioning

confidence: 87%

“…The petroleum ether, ethyl acetate and dichloromethane fractions presented IC 50 of 74.35, 79.73 and 57.30 µg/mL, respectively. Dichloromethane fraction still present strong antimicrobial activity, with clear zone of 23, 22, 22 and 21 (ϕmm) on Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger, respectively (Abdel-Aziz et al, 2021).…”

Section: Ceiba Speciosamentioning

confidence: 97%

Genus Ceiba Mill. in Brazil: A comprehensive review on its ethnopharmacology, phytochemistry and bioactivities

Silva¹,

Silva²,

Cunha³

et al. 2023

NRFHH

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Introduction: Neotropical genus Ceiba Mill. is known for having tall trees, trunks with a robust part and fruits of economic interest. In Brazil, there are eleven native species, from those ﬁve are endemic. It is used in folk medicine to treat diabetes, inﬂammation, pain and diarrhea; however, most of the species have no scientiﬁc validation for such activities. This review aims to compile information about the genus Ceiba in Brazil, to update and allow an integrated understanding of its medicinal uses, chemical composition, and biological activities. Methodology: Ceiba species reported in Flora e Funga do Brasil were used for this literature review, using ScienceDirect, PubMed, and Google Scholar as articles databases. Results: In traditional use, the most cited species was C. pentandra (25 citations), the most used part was stem/bark (30 citations), preparation method was decoction (19 citations), and main administration route was oral ingestion (12 reports) for digestive system, skin and subcutaneous tissues diseases treatment. Genus chemical composition is wide, presenting metabolites such as proteins, sugars, fatty acids, tannins, ﬂavonoids, and alkaloids. Bioactivities as antidiabetic, antioxidant, antitumor and antimicrobial were observed, especially for C. pentandra. Conclusion: Some species of Ceiba have extensive scientiﬁc literature, presenting several isolated compounds and bioactivities. On the other hand, some species, especially those that are endemics only in Brazil, do not have studies that evaluate their biological properties. Such knowledge is essential to conﬁrm the medicinal potential cited in ethnobotanical reports, and the possibility of new compounds of biotechnological interest

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“…The identification and estimation of polyphenolic compounds of PPE and Alg-PPE NPs by HPLC were carried out according to the method described by Abdel-Aziz et al [24] . The mobile phase consists of solution A (HPLC grade water) and solution B (0.05 % trifluoracetic acid in acetonitrile).…”

Section: Identification Of Polyphenolic Compounds Of Pee and Alg-ppe ...mentioning

confidence: 99%

Protective effect of Punica granatum peel extract and its alginate-encapsulated nanoparticles against acrylamide-induced neurotoxicity in rats

Tawfek,

El-Houssiny,

Ahmed-Farid

et al. 2023

Egyptian Journal of Pure and Applied Science

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The goal of the present study is to assess the possible protective effect of the pomegranate peel extract (PPE) and its alginate-nanoencapsulated form (Alg-PPE NPs) against rat neurotoxicity induced by acrylamide (ACR). The prepared Alg-PPE NPs were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential, Fourier transmission infrared microscopy (FITR), differential scanning calorimetry (DSC) as well as in vitro cytocompatibility and drug release analysis. The active ingredients of the two examined extracts were identified using HPLC analysis. ACR was intraperitoneally injected (50 mg/kg bw) three times per week for 2 consecutive weeks into male Wistar rats for the induction of neurotoxicity. PPE or Alg-PPE NPS were given in daily doses (200 mg/kg bw) orally for two consecutive weeks either individually or combined with acrylamide injection. Results revealed that ACR injection induced neurotoxicity manifested by the significant increase in tumor necrosis factor alpha, gamma aminobutyric acid, and DNA fragmentation level in brain tissues of treated animals. Meanwhile, the brain levels of brainderived neurotrophic factor, total antioxidant capacity, acetylcholinesterase activity, dopamine, serotonin, norepinephrine, glutamine, glycine, and aspartate were significantly decreased. These changes in tissue biochemical parameters were associated with histopathological alternations in the architecture of brain tissues. Amelioration of the acrylamide-induced brain toxicity was observed following co-administration of either PPE or Alg-PPE NPs with a higher degree of protection observed with the nanoparticles form as a result of increased bioavailability. The protective effect of the studied extracts was ascribed mainly to the antioxidant potential of their phenolic and flavonoids components. In conclusion, Alg-PPE NPs can be regarded as a potential therapeutic agent that offers protection against acrylamide neurotoxicity, however, to fully understand the neuroprotective mechanism of Alg-PPE NPs at the molecular level, additional research must be done to characterize their active principles.

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High‑Performance Liquid Chromatography‑Fingerprint Analyses, In vitro Cytotoxicity, Antimicrobial and Antioxidant Activities of the Extracts of Ceiba speciosa Growing in Egypt

Cited by 12 publications

References 14 publications

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Genus <i>Ceiba</i> Mill. in Brazil: A comprehensive review on its ethnopharmacology, phytochemistry and bioactivities

Protective effect of Punica granatum peel extract and its alginate-encapsulated nanoparticles against acrylamide-induced neurotoxicity in rats

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