High Performance Liquid Chromatography Fluorescence Method for the Determination of Seleno-Amino Acids in Ovine Blood Plasma

Abstract

This study was performed to validate an effective high performance liquid chromatographyfluorescence detection method for the separation of seleno-amino acids and sulfur amino acids in biological samples using pre-column derivatization with 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate. Standard calibration and standard addition procedures were developed, but the quantification of seleno-amino acids was carried out by a second method. The coefficient of variation for precision was < 15% and accuracies were 96 to 98%. Recoveries in the extraction method were between 63 and 99%. This method was applied to the contribution of seleno-amino acids containing methylselenocysteine and selenomethionine in plasma tissue of lambs during infusion study. The method demonstrated good selectivity, sensitivity, precision, and accuracy.

Extraction of Seleno-Amino Acids from <i>Cardamine Hupingshanensis</i> by Ultrasonic Assisted Deep Eutectic Solvents Extraction

“…Compared with selenoprotein, seleno-amino acids are more easily absorbed and utilized by the human body. Free seleno-amino acids in selenium-rich products are often extracted with hydrochloric acid as the solvent and analyzed by HPLC [14,15]. However, according to the principle of green chemistry, toxic and harmful solvents should be avoided as far as possible, so it is necessary to develop greener extraction solvents.…”