High-Performance Liquid Chromatography for Analysis of Herbal Drugs

Mukherjee, Pulok K.

doi:10.1016/b978-0-12-813374-3.00010-7

Cited by 5 publications

(4 citation statements)

References 131 publications

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“…The optimization of methodologies and conditions was a critical step in devising a successful strategy. According to the literature, the Reversed-Phase Flash Chromatography (RP-FC) system is effectively used for the analysis and separation of polyphenolic structures, facilitating the separation of a broad spectrum of solutes with medium to high polarity that exhibit diverse interactions with the octadecylsilane of the stationary phase [ 5 , 29 ]. When the technique was applied with the transposition of the analytical method, no effective chromatographic separation of any individual polyphenol in the EAF was observed ( Figure S2A ).…”

Section: Resultsmentioning

confidence: 99%

“…Most of the tests consider the development and application of combined methodologies within the world of chromatography [3,4]. The recovery of these constituents is desirable for the structural Molecules 2024, 29, 2761 2 of 21 and pharmacological elucidation of bioactives, contributing to the collection of information available for this class of metabolites [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Tenório,

Dantas,

Abreu

et al. 2024

Molecules

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The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex® LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS2) and subjected to the microdilution method in 96-well plates against strains of C. albicans, C. auris, and C. glabrata. Myricitrin (93.89%; w/w; m/z 463.0876), gallic acid (99.9%; w/w; m/z 169.0142), and ellagic acid (94.2%; w/w; m/z 300.9988) were recovered. The polyphenolic fraction (62.67% (w/w) myricitrin) and the ellagic fraction (67.86% (w/w) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 μg/mL), suggesting an association between the majority constituents and the antifungal response of E. uniflora derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Tenório,

Dantas,

Abreu

et al. 2024

Molecules

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“…Lignans are among the phenolic components that are widely distributed in the plant kingdom and identified in various parts of over 60 plant families [ 24 , 25 , 26 ]. Structurally, lignans consist of two phenylpropane units linked by β,β-bonds ( Figure 1 ) [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…Lignans are among the phenolic components that are widely distributed in the plan kingdom and identified in various parts of over 60 plant families [24][25][26]. Structurally lignans consist of two phenylpropane units linked by β,β-bonds (Figure 1) [27]. These metabolites possess various positive health effects, such as antiplatelet aggregation; antimicrobial, antiviral, antioxidant, anti-estrogenic, and antimutagenic activities; and marked anticancer activity against various cancerous cells [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking and Molecular Dynamics Studies Reveal the Anticancer Potential of Medicinal-Plant-Derived Lignans as MDM2-P53 Interaction Inhibitors

Shoaib,

Abdelmoniem,

Mukhtar

et al. 2023

Molecules

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The interaction between the tumor suppressor protein p53 and its negative regulator, the MDM2 oncogenic protein, has gained significant attention in cancer drug discovery. In this study, 120 lignans reported from Ferula sinkiangensis and Justicia procumbens were assessed for docking simulations on the active pocket of the MDM2 crystal structure bound to Nutlin-3a. The docking analysis identified nine compounds with higher docking scores than the co-crystallized reference. Subsequent AMDET profiling revealed satisfactory pharmacokinetic and safety parameters for these natural products. Three compounds, namely, justin A, 6-hydroxy justicidin A, and 6′-hydroxy justicidin B, were selected for further investigation due to their strong binding affinities of −7.526 kcal/mol, −7.438 kcal/mol, and −7.240 kcal/mol, respectively, which surpassed the binding affinity of the reference inhibitor Nutlin-3a (−6.830 kcal/mol). To assess the stability and reliability of the binding of the candidate hits, a molecular dynamics simulation was performed over a duration of 100 ns. Remarkably, the thorough analysis demonstrated that all the hits exhibited stable molecular dynamics profiles. Based on their effective binding to MDM2, favorable pharmacokinetic properties, and molecular dynamics behavior, these compounds represent a promising starting point for further refinement. Nevertheless, it is essential to synthesize the suggested compounds and evaluate their activity through in vitro and in vivo experiments.

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Synthesis and characterisation of porous polyvinylpyrrolidone-co-poly(divinylbenzene) (PVP-co-PDVB) copolymer resins for the isolation of betalains from red beetroot

2020

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High-Performance Liquid Chromatography for Analysis of Herbal Drugs

Cited by 5 publications

References 131 publications

Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation

Molecular Docking and Molecular Dynamics Studies Reveal the Anticancer Potential of Medicinal-Plant-Derived Lignans as MDM2-P53 Interaction Inhibitors

Synthesis and characterisation of porous polyvinylpyrrolidone-co-poly(divinylbenzene) (PVP-co-PDVB) copolymer resins for the isolation of betalains from red beetroot

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