High-performance liquid chromatography LTQ-Orbitrap mass spectrometry method for tomatidine and non-target metabolites quantification in organic and normal tomatoes

Abstract

Tomatoes, members of the Solanaceae plant family, produce biologically active secondary metabolites, including glycoalkaloids and aglycons, which may have both adverse and beneficial biological effects. A new liquid chromatography method that utilized LTQ-Orbitrap MS was developed for the analysis of tomatidine, the main aglycon in tomatoes. Recoveries of tomatidine were >98.3% with the relative standard deviations (RSDs) below 6.1%. The limit of detection (LODs) was 0.0003 mg kg(-1). The limit of quantitation (LOQs) is 0.001 mg kg(-1). The linear range was between with 0.0025 and 1 mg kg(-1) with an excellent correlation coefficient (R(2)) equal to 0.9990. Various tomato samples were analyzed and the level of tomatidine in the 11 samples analysed was higher in normal respect to organic tomatoes. The capability of the set-up Full Scan LTQ-Orbitrap MS method allowed us to quantified two non-target analytes. The m/z 1032 was identified as dehydrotomatine, confirmed through accurate mass studies (mass error in ppm equal to 1.5017) meanwhile m/z 902 as (Glc)2-Gal-Tomatidine (β1-Tomatine) (mass error in ppm equal to 2.0719).

Chemistry and Anticarcinogenic Mechanisms of Glycoalkaloids Produced by Eggplants, Potatoes, and Tomatoes

“…A related study found that the tomatidine content of 11 analyzed samples was higher in conventional tomatoes than that in organic tomatoes. 29 These results suggest the possibility of future anticancer and anticholesterol studies with selected high-tomatine and high-tomatidine red tomatoes.…”

Chemistry, Nutrition, and Health-Promoting Properties of Hericium erinaceus (Lion’s Mane) Mushroom Fruiting Bodies and Mycelia and Their Bioactive Compounds

“…The results imply that the novel polysaccharide might be a candidate for treating gastric cancer. The use of HEG-5 against human gastric cancer is expected to be successful in light of the fact that H. erinaceus mushrooms are reported to prevent gastric ulcers, chronic gastritis, and other digestive tract-related diseases. , …”

Identification and quantification of new isomers of isopropyl-malic acid in wine by LC-IT and LC-Q-Orbitrap

“…On the other side, for the identification of unknown compounds, the combination of Fourier Transform Orbitrap MS technology with a linear ion trap MS has been shown to give excellent results as it allowed identification and quantitation in untargeted and targeted analysis (Caprioli, Cahill & James, 2014a;Caprioli, Logrippo, Cahill & James, 2014b). Thus, the first aim of this work was to characterize the unidentified compound in wines with a nominal molecular mass of 176 u (Ginjom et al 2011;Monagas et al 2005) by using two LC-MS platforms, i.e.…”