High-Performance Liquid Chromatography with Chemiluminescence Detection of Methamphetamine and Amphetamine in Human Urine

Nakashima, Kenichiro; Suetsugu, Keiko; Yoshida, Kazunori; Imai, Kazuhiro; Akiyama, Shuzo

doi:10.2116/analsci.7.815

Cited by 27 publications

(9 citation statements)

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“…Recently, we have been aiming at developing selective and sensitive analytical procedures for the determination of these compounds in biological samples (Nakashima et al, 1990(Nakashima et al, , 1991(Nakashima et al, , 1992Al-Dirbashi et al, 1997;Kuroda et al, 1998). Higher sensitivities with simple sample pretreatment and derivatization procedures could be attained when MP and its related compounds were analyzed in biological samples either on a reversed-phase or a chiral stationary phase packed column (Al-Dirbashi et al, 1998a, b, 1999a as derivatives of our previously developed fluorescence labeling reagent, namely, 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride (DIB-Cl) (Nakashima et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Al-Dirbashi

Kuroda

Wada

et al. 2000

Biomed. Chromatogr.

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Achiral and chiral semi-micro column high-performance liquid chromatographic methods with fluorescence detection to determine methamphetamine and amphetamine in human hair are described. These compounds were extracted into 5% trifluoroacetic acid (TFA) in methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride and separated either on a 250 x 1.5 mm i.d. octadecyl-silane (ODS) or a 150 x 2 mm i.d. OD-RH column. Linear calibration curves extending over a wide range of concentration that covers the practical samples were obtained for amphetamine, methamphetamine and their enantiomers (r = 0.999). Resolution values for amphetamine and methamphetamine enantiomers were 3.4 and 1.1, respectively. Intra- and inter-day variations of both the methods were not larger than 8.9% expressed as relative standard deviations (n >/= 5). The limits of detection at a signal-to-noise ratio of 3 obtained by both the methods were in the range of 1.0-4.7 fmol/5 microL injection with the achiral method being more sensitive. Abusers' hair samples were analyzed by the two methods and only the S(+)-enantiomers were found in eight Japanese abusers' hair samples. The achiral method was used to study the concentrations of these compounds in single black and white hair strands of abusers.

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Section: Introductionmentioning

confidence: 99%

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Al-Dirbashi

Kuroda

Wada

et al. 2000

Biomed. Chromatogr.

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“…Utilizing the former derivatization method, MA and its five metabolites in urine were separately determined (Hayakawa et al, 1993). N-(4-Aminobuty1)-N-ethyl-isoluminol (Nakashima et al, 1991) and 4-(NJV-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (Nakashima et al, 1992) were also used as other derivatization reagents for the simultaneous determination of MA and its metabolites. In this study, we applied the CL-HPLC method using dansyl derivatives for the sensitive determination of MA and AP in hair samples and confirmed that its sensitivity was higher than that of GCMS using selective ion monitoring (SIM).…”

Section: Introductionmentioning

confidence: 99%

Determination of Stimulants in a Single Human Hair Sample by High-Performance Liquid Chromatographic Method with Chemiluminescence Detection

Takayama

Tanaka

Hayakawa

1997

Biomed. Chromatogr.

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Stimulants that are controlled by the Stimulant Drug Control Law of Japan are methamphetamine (MA) and amphetamine (AP). MA is used by most stimulant addicts, and AP is detected as its main metabolite. We have developed a high‐performance liquid chromatographic method with chemiluminescence detection (CL‐HPLC), for determining trace levels of MA and its metabolites in a single human hair sample, in which bis(2,4,6‐trichlorophenyl)oxalate and hydrogen peroxide are the postcolumn reagents. After washing a single hair sample with water and methanol, it was cut into pieces, extracted with a mixed solution of methanol and hydrochloric acid for 1 h under ultra‐sonication and allowed to stand at room temperature overnight. Then the organic phase was evaporated to dryness. To the residues, 0.1 mL of carbonate buffer and 0.1 mL of dansyl chloride solution were added and the solution was heated at 45°C for 1 h. An aliquot of the reaction mixture was then subjected to HPLC. MA and AP were chemiluminogenically detected as their dansyl derivatives from a sample of only a single hair. The detection limit was about 2 pg in an injected volume (20 μl), and about 20 pg in a single hair sample. This detection limit was smaller than that by the gas chromatography/mass spectrometry (selective ion monitoring) method. Our method was useful as a screening test for stimulant users. © 1997 by John Wiley & Sons, Ltd.

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“…2,3 It was applied to the ultra trace analysis of catecholamines 26 , amino acids [27][28][29][30] , steroids [31][32][33] and drugs. 3,[34][35][36][37][38][39][42][43][44][45][46] …”

Section: ·3 Development Of the Methodsmentioning

confidence: 99%

An Invitation to Bio-Analytical Chemistry

Imai

1998

ANAL. SCI.

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High-Performance Liquid Chromatography with Chemiluminescence Detection of Methamphetamine and Amphetamine in Human Urine

Cited by 27 publications

References 10 publications

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses

Determination of Stimulants in a Single Human Hair Sample by High-Performance Liquid Chromatographic Method with Chemiluminescence Detection

An Invitation to Bio-Analytical Chemistry

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