High-Performance Membrane Chromatography. A Novel Method of Protein Separation

Tennikova, Tatiana; Švec, F.; Belen'kii, B.G.

doi:10.1080/01483919008051787

Cited by 340 publications

(155 citation statements)

References 6 publications

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“…Organic polymer monoliths, first reported by the group of Svec in the early 1990s [5,6], have the major advantages of simplicity of preparation, versatile surface modification, and high chemical stability over a broad range of pH. Polymer stationary phases can be prepared in a single step by the copolymerization of functional monomers having potential chromatographic selector [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

Sun

Chen

et al. 2010

Anal Bioanal Chem

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Two types of macroporous organic polymer monoliths based on glycidyl methacrylate (GMA), 4-vinylbenzyl chloride (VBC) and divinylbenzene (DVB) were prepared inside stainless-steel tubes. Azide functionalities were firstly introduced on the surfaces of poly(GMAco-DVB) and poly(VBC-co-DVB) monoliths to provide reactive sites for click chemistry. With the application of copper(I)-catalyzed (3+2) azide-alkyne cycloaddition, an in-column click-modification approach for covalent attachment of long alkyl chains onto polymer monoliths was developed. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by the scanning electron microscopy, mercury intrusion porosimeter, Fourier transform infrared spectroscopy, and elemental analyses, respectively. The chromatographic performances of the "clicked" stationary phases were demonstrated with the high separation efficiency for a variety of proteins within 4 min.

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Section: Introductionmentioning

confidence: 99%

In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

Sun

Chen

et al. 2010

Anal Bioanal Chem

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“…Discs cut from this monolithic polymer showed high permeability allowing fast separations of proteins (Tennikova et al, 1990). Since then, a multitude of studies have been carried out, and have made significant impacts on the separation technology.…”

Section: Organic Monolithmentioning

confidence: 99%

Green biodiesel production: a review on feedstock, catalyst, monolithic reactor, and supercritical fluid technology

et al. 2016

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Please cite this article as: Gumba R.E., Saallah S., Misson M., Ongkudon C.M., Anton A. Green biodiesel production: a review on feedstock, catalyst, monolithic reactor and supercritical fluid technology. Biofuel Research Journal 11 (2016) The advancement of alternative energy is primarily catalyzed by the negative environmental impacts and energy depletion caused by the excessive usage of fossil fuels. Biodiesel has emerged as a promising substitute to petrodiesel because it is biodegradable, less toxic, and reduces greenhouse gas emission. Apart from that, biodiesel can be used as blending component or direct replacements for diesel fuel in automotive engines. A diverse range of methods have been reported for the conversion of renewable feedstocks (vegetable oil or animal fat) into biodiesel with transesterification being the most preferred method. Nevertheless, the cost of producing biodiesel is higher compared to fossil fuel, thus impeding its commercialization potentials. The limited source of reliable feedstock and the underdeveloped biodiesel production route have prevented the full-scale commercialization of biodiesel in many parts of the world. In a recent development, a new technology that incorporates monoliths as support matrices for enzyme immobilization in supercritical carbon dioxide (SC-CO2) for continuous biodiesel production has been proposed to solve the problem. The potential of SC-CO2 system to be applied in enzymatic reactors is not well documented and hence the purpose of this review is to highlight the previous studies conducted as well as the future direction of this technology. © 2016 BRTeam. All rights reserved.Journal homepage: www.biofueljournal.com Research Unit, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, 88400, Malaysia. Gumba et al. / Biofuel Research Journal 11 (2016) [431][432][433][434][435][436][437][438][439][440][441][442][443][444][445][446][447] Please cite this article as: Gumba R.E., Saallah S., Misson M., Ongkudon C.M., Anton A. Green biodiesel production: a review on feedstock, catalyst, monolithic reactor and supercritical fluid technology. Energy

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“…Monoliths are the fourth generation chromatography material [3], succeeding beads, porous particle-based columns and membrane adsorbers. In the last two decades, numerous articles describing preparation, properties and applications of monolithic columns were published [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. In contrast, this review focuses on the applied aspect of monoliths and describes recent applications of monoliths for the purification of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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High-Performance Membrane Chromatography. A Novel Method of Protein Separation

Cited by 340 publications

References 6 publications

In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation

Green biodiesel production: a review on feedstock, catalyst, monolithic reactor, and supercritical fluid technology

Monoliths in Bioprocess Technology

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